Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A18 F30 I1 R1	22	28.4	958084	99.2%	950419	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,007	C→A	A341E (GCG→GAG)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,007	0	C	A	100.0%	122.8 / NA	36	A341E (GCG→GAG)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/20); total (16/20)

TAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCG  >  NC_000913/421879‑422139

tAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTg                                                                                                                           <  2:2066/140‑1 (MQ=255)

  aCAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCg                                                                                                                         <  2:285662/140‑1 (MQ=255)

   cAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCgg                                                                                                                        <  2:54082/140‑1 (MQ=255)

    aGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgt                                                                                                                       <  2:8535/140‑1 (MQ=255)

    aGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgt                                                                                                                       <  2:214469/140‑1 (MQ=255)

      tGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGgtgt                                                                                                                     >  1:169811/1‑140 (MQ=255)

       gATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTa                                                                                                                    >  1:16402/1‑140 (MQ=255)

         tGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGACGACTACCGAGCTGCTGTTTGCGGTGTATc                                                                                                                  >  2:217365/1‑140 (MQ=255)

           tATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTg                                                                                                                <  1:382388/140‑1 (MQ=255)

             tttGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGaa                                                                                                              >  2:196294/1‑140 (MQ=255)

                ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTTGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTa                                                                                                           >  2:105846/1‑140 (MQ=255)

                ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCg                                                                                                                         <  1:190906/126‑1 (MQ=255)

                ggCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGACGACTACCGAGCTGCTGTTTGCg                                                                                                                         >  2:190906/1‑126 (MQ=255)

                       gCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATg                                                                                                    >  2:12083/1‑140 (MQ=255)

                       gCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATg                                                                                                    >  2:163426/1‑140 (MQ=255)

                           tATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCgg                                                                                                >  2:251434/1‑140 (MQ=255)

                                 ccACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACg                                                                                          <  1:12083/140‑1 (MQ=255)

                                   aCGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTc                                                                                        <  2:197905/140‑1 (MQ=255)

                                         tGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTg                                                                                  >  2:428082/1‑140 (MQ=255)

                                               agCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGAtc                                                                            >  1:81661/1‑140 (MQ=255)

                                                    ggCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGAtcgcttc                                                                       >  2:194628/1‑140 (MQ=255)

                                                         cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  <  1:310764/140‑1 (MQ=255)

                                                         cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  1:306433/1‑140 (MQ=255)

                                                         cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  2:12743/1‑140 (MQ=255)

                                                         cgcgcCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTgg                                                                  >  2:381283/1‑140 (MQ=255)

                                                                     tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  2:285293/140‑1 (MQ=255)

                                                                     tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  1:163426/140‑1 (MQ=255)

                                                                     tttCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCa                                                      <  2:306433/140‑1 (MQ=255)

                                                                          gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtg                                                 <  1:12743/140‑1 (MQ=255)

                                                                          gCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtg                                                 <  2:169811/140‑1 (MQ=255)

                                                                           cAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgt                                                <  2:268542/140‑1 (MQ=255)

                                                                               aCGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggt                                            <  1:304578/140‑1 (MQ=255)

                                                                                cgtcgcgtcgCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggtg                                           <  2:451043/140‑1 (MQ=255)

                                                                                  tcgcgtcgcgGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACggtgtgggtgtg                                         <  2:81661/140‑1 (MQ=255)

                                                                                                   gTGGGTTACGGTGCTGGTGATGACTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGt                        <  1:105846/140‑1 (MQ=255)

                                                                                                                         aCTACCGAGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCg  >  1:273228/1‑140 (MQ=255)

TAACAGTGATGTATTTGGCGTAGGCCGTATGCTCCACGGTATGGCAGAGCAGGGCAGCGCGCCGAAAATTTTCAGCAAAACGTCGCGTCGCGGTATTCCGTGGGTTACGGTGCTGGTGATGACTACCGCGCTGCTGTTTGCGGTGTATCTGAACTACATCATGCCGGAAAACGTCTTCCTGGTGATCGCTTCGCTGGCAACCTTCGCCACGGTGTGGGTGTGGATTATGATCCTGCTGTCGCAAATTGCCTTCCGTCGCCG  >  NC_000913/421879‑422139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A