Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F30 I1 R1	21	82.8	2811760	99.1%	2786454	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,388,316	T→A	D352V (GAT→GTT)	mscM ←	miniconductance mechanosensitive channel MscM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,388,316	0	T	A	100.0%	242.7 / NA	70	D352V (GAT→GTT)	mscM	miniconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (33/37); total (33/37)

CCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGC  >  NC_000913/4388181‑4388444

ccACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTc                                                                                                                              >  1:1321968/1‑140 (MQ=255)

ccACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTc                                                                                                                              <  1:138897/140‑1 (MQ=255)

 cACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCa                                                                                                                             <  2:1358105/140‑1 (MQ=255)

       gCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGt                                                                                                                       <  2:909913/140‑1 (MQ=255)

        caacaaTGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTa                                                                                                                      >  2:112704/1‑140 (MQ=255)

             aTGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGt                                                                                                                 >  2:526124/1‑140 (MQ=255)

             aTGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGt                                                                                                                 >  2:653293/1‑140 (MQ=255)

              tGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCt                                                                                                                               >  2:178502/1‑125 (MQ=255)

              tGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCt                                                                                                                               <  1:178502/125‑1 (MQ=255)

                  ttCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGcac                                                                                                            >  2:1306521/1‑140 (MQ=255)

                  ttCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGcac                                                                                                            >  1:465159/1‑140 (MQ=255)

                     aGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGtt                                                                                                                >  2:478883/1‑133 (MQ=255)

                     aGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGtt                                                                                                                <  1:478883/133‑1 (MQ=255)

                          cTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACt                                                                                                    >  1:566694/1‑140 (MQ=255)

                                   aGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTgcg                                                                                                 >  1:1272320/1‑134 (MQ=255)

                                   aGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTgcg                                                                                                 <  2:1272320/134‑1 (MQ=255)

                                        gcAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTa                                                                                      <  2:566694/140‑1 (MQ=255)

                                         cAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTAt                                                                                     >  2:336544/1‑140 (MQ=255)

                                           gTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCa                                                                                   <  1:1306521/140‑1 (MQ=255)

                                            ttGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCaa                                                                                  <  2:465159/140‑1 (MQ=255)

                                             tgtgCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAg                                                                                 <  1:336544/140‑1 (MQ=255)

                                             tgtgCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAg                                                                                 <  1:1089120/140‑1 (MQ=255)

                                             tgtgCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAg                                                                                 >  2:447423/1‑140 (MQ=255)

                                               tgCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCt                                                                               >  2:319537/1‑140 (MQ=255)

                                                   tCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCtgttg                                                                           <  1:112704/140‑1 (MQ=255)

                                                    ccAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCtgttgt                                                                          >  1:145426/1‑140 (MQ=255)

                                                    ccAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCtgttgt                                                                          >  2:375107/1‑140 (MQ=255)

                                                         aTACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGttt                                                                     >  2:564681/1‑140 (MQ=255)

                                                           aCGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCg                                                                   <  1:375107/140‑1 (MQ=255)

                                                            cGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCgg                                                                  >  1:1208142/1‑140 (MQ=255)

                                                             ggTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGc                                                                 >  1:297181/1‑140 (MQ=255)

                                                             ggTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGc                                                                 >  2:866025/1‑140 (MQ=255)

                                                             ggTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGc                                                                 >  2:713000/1‑140 (MQ=255)

                                                               ttttGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCAt                                                               >  1:1319110/1‑140 (MQ=255)

                                                                  tGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTc                                                            >  2:1390113/1‑140 (MQ=255)

                                                                    cTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCg                                                          >  1:494520/1‑140 (MQ=255)

                                                                             cAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTg                                                 <  2:297181/140‑1 (MQ=255)

                                                                                     gACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTgc                                         <  2:1321968/140‑1 (MQ=255)

                                                                                      aCCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTgcg                                        <  1:866025/140‑1 (MQ=255)

                                                                                      aCCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTgcg                                        >  2:66619/1‑140 (MQ=255)

                                                                                          tCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCg                                    <  2:371985/140‑1 (MQ=255)

                                                                                          tCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCg                                    >  2:109218/1‑140 (MQ=255)

                                                                                                 gatgaATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCt                             >  1:387763/1‑140 (MQ=255)

                                                                                                 gatgaATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCt                             >  1:1033311/1‑140 (MQ=255)

                                                                                                 gatgaATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCt                             <  1:653293/140‑1 (MQ=255)

                                                                                                       tttGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCg                       >  2:18305/1‑140 (MQ=255)

                                                                                                         tGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAg                     <  2:145426/140‑1 (MQ=255)

                                                                                                         tGCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAg                     <  1:447423/140‑1 (MQ=255)

                                                                                                          gCCGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGc                    >  1:759168/1‑140 (MQ=255)

                                                                                                            cGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAg                  >  1:1067337/1‑140 (MQ=255)

                                                                                                            cGTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAg                  >  2:1023136/1‑140 (MQ=255)

                                                                                                             gTAGCAGCGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGa                 <  1:713000/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:977294/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  2:494520/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  2:1220070/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:989606/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:1020469/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:725192/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:526124/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:370807/140‑1 (MQ=255)

                                                                                                                  agcGGCTGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  1:319537/140‑1 (MQ=255)

                                                                                                                        tGTTTATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATcc      <  1:1023136/140‑1 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAg                     >  1:598111/1‑123 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAg                     <  2:598111/123‑1 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGAt                <  2:553344/128‑1 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCa    <  2:759168/140‑1 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCa    <  1:18305/140‑1 (MQ=255)

                                                                                                                          tttATTAAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAACCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGAt                >  1:553344/1‑128 (MQ=255)

                                                                                                                            tattaAGCAGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGc  <  1:66619/140‑1 (MQ=255)

                                                                                                                                    aGAACCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGa            <  2:649452/122‑1 (MQ=255)

CCACCCTGCAACAATGAGTTCAGCAACTCACGCTGAGTGCGCAGTTGTGCTTCCAGAATACGGTTTTGCTCGGCAGTCAGCGGCTGACCGTCGGCCTGATGAATTTGCCGTAGCAGCGGCTGTTTATTAAGCAGATCCTCATAACGTAACCGTTGCACACGCAACTGCGCCATTTCGGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGC  >  NC_000913/4388181‑4388444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A