breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 70,789 G→T G135W (GGG→TGG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 97,750 C→A R222S (CGC→AGC)  murD → UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 824,694 T→G L22* (TTA→TGA)  ybhQ → putative inner membrane protein
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,496,597 C→A intergenic (+32/‑324) lpxP → / → ypdK palmitoleoyl acyltransferase/putative membrane protein YpdK
RA 2,692,546 C→A E1000* (GAG→TAG)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,942,516 C→A A18S (GCA→TCA)  gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 3,163,674 G→T intergenic (‑193/+41) plsC ← / ← parC 1‑acylglycerol‑3‑phosphate O‑acyltransferase/DNA topoisomerase IV subunit A
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,388,316 T→A D352V (GAT→GTT)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,570,380 C→A G399* (GGA→TGA)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102981 3104069 1089 24 [22] [20] 25 mutY mutY
* * ÷ NC_000913 3423752–3424234 3424538–3424239 6–787 23 [22] [22] 24 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)46 (0.810) 19/190 1.6 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375102 =NA (NA)35 (0.540) 15/216 2.7 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 375201NA (NA)26 (0.370) 16/238 2.8 NA noncoding (19/36 nt) other REP31f
?NC_000913 = 377352 NA (NA)noncoding (17/37 nt) other REP32a
* ? NC_000913 375203 =NA (NA)46 (0.720) 22/216 1.6 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 1207790 =11 (0.130)88 (1.210) 51/244 0.2 91.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 7 (0.100)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780511 (0.130)46 (0.630) 35/244 0.8 84.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 7 (0.100)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =119 (1.440)114 (1.390) 60/274 0.2 65.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)89 (1.080) 48/276 0.5 97.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA