Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	228	65.7	2434974	91.7%	2232871	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,313,070	T→C	T37A (ACC→GCC)	rbfA ←	30S ribosome binding factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,313,070	0	T	C	100.0%	132.8 / NA	38	T37A (ACC→GCC)	rbfA	30S ribosome binding factor
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/19); total (19/19)

TGCGGATGAAACCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGG  >  NC_000913/3312938‑3313202

tGCGGATGAAACCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGtcatc                                                                                                                               >  2:1027425/1‑140 (MQ=255)

    gATGAAACCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGc                                                                                                                           <  2:1141803/140‑1 (MQ=255)

     aTGAAACCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGcc                                                                                                                          <  2:655971/140‑1 (MQ=255)

        aaaCCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAg                                                                                                                       >  2:51313/1‑140 (MQ=255)

              gAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAgg                                                                                                                 <  1:291931/140‑1 (MQ=255)

              gAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAgg                                                                                                                 <  1:51313/140‑1 (MQ=255)

                     cttGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTa                                                                                                          >  2:708299/1‑140 (MQ=255)

                        gCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAAtt                                                                                                       <  2:7609/140‑1 (MQ=255)

                            cGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCAc                                                                                                   <  2:586017/140‑1 (MQ=255)

                                               aCCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGAt                                                                                <  1:756427/140‑1 (MQ=255)

                                                ccGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGAtc                                                                               <  1:607790/140‑1 (MQ=255)

                                                   cgTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATctct                                                                            >  1:503909/1‑140 (MQ=255)

                                                       ttCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCCGGCGAGGAGCTTTTATTTTACGCCGCAGGGTGAGAGCGGTCTCttttt                                                                        >  2:607790/1‑140 (MQ=255)

                                                               tGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGACACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTcc                                                                >  1:1164906/1‑140 (MQ=255)

                                                                gTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCt                                                               <  2:319126/140‑1 (MQ=255)

                                                                gTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCt                                                               >  2:700792/1‑140 (MQ=255)

                                                                               cacaTATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCg                                                <  1:1159771/140‑1 (MQ=255)

                                                                                 caTATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCggg                                              >  1:166226/1‑140 (MQ=255)

                                                                                      aCTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCggg                                              >  1:665651/1‑135 (MQ=255)

                                                                                      aCTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCggg                                              <  2:665651/135‑1 (MQ=255)

                                                                                         ttGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCaaa                                      >  2:294224/1‑140 (MQ=255)

                                                                                                aCGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTc                               >  1:610564/1‑140 (MQ=255)

                                                                                                 cGCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCg                              >  2:200914/1‑140 (MQ=255)

                                                                                                  gCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGc                             >  1:554627/1‑140 (MQ=255)

                                                                                                  gCCAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGc                             <  1:16928/140‑1 (MQ=255)

                                                                                                   ccAGGTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGcc                            <  2:503909/140‑1 (MQ=255)

                                                                                                      ggTCGCGAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCata                         >  1:997967/1‑140 (MQ=255)

                                                                                                           cgAGGCATTTCGACACCGGAAACGGCGGCCCTCATGGCCACGTGAAGATCTTTAAATTCACCGTGCTGGATTGATGGGGTTTCTTTTTACATTTACTGCGCTAGCCGCTCCGGGGGAGCCAATTATTTACCCCTAATaaa                    >  2:479023/1‑140 (MQ=255)

                                                                                                           cgAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCttt                                <  1:723270/128‑1 (MQ=255)

                                                                                                           cgAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCttt                                >  2:723270/1‑128 (MQ=255)

                                                                                                           cgAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATaaa                    >  2:998693/1‑140 (MQ=255)

                                                                                                           cgAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATaaa                    >  1:246032/1‑140 (MQ=255)

                                                                                                           cgAGACATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCA‑TTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAAt                   >  1:368905/1‑140 (MQ=255)

                                                                                                                cATTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATtctc               <  1:700792/140‑1 (MQ=255)

                                                                                                                 aTTTCGACACCGGAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTcc              >  1:687459/1‑140 (MQ=255)

                                                                                                                   ttCGACACCGGAAACGGCGGTCATCATGCCAAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGGTCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTg            <  2:785514/140‑1 (MQ=255)

                                                                                                                             gAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATtctc               >  2:1107047/1‑127 (MQ=255)

                                                                                                                             gAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATtctc               <  1:1107047/127‑1 (MQ=255)

                                                                                                                             gAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAgg  <  1:294224/140‑1 (MQ=255)

                                                                                                                             gAAACGGCGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAgg  <  2:898537/140‑1 (MQ=255)

TGCGGATGAAACCAGAAGCTTCTTGCAACGCTTTGATGCCCGCTTTAACCGCGTCTTCATCTTTGTCGTTGAGGAACGTCACATATACTTTGGCATACGCCAGGTCGCGAGACATTTCGACACCGGAAACGGTGGTCATCATGCCCAGGCGAGGATCTTTAATTTCACGCTGCAGGATGAGAGCGATCTCTTTTTGCATTTCCTGCGCTACGCGCTGCGGGCGACCAAATTCTTTCGCCATAATAAATTCTCCTGACAAAAAAGG  >  NC_000913/3312938‑3313202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A