breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 496 (A)6→7 coding (160/2463 nt) thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 1,152 (C)5→6 coding (816/2463 nt) thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 1,875 A→G V513V (GTA→GTG thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 51,317 C→T G97D (GGT→GAT)  apaG ← DUF525 domain‑containing protein ApaG
RA 66,090 (T)5→4 coding (461/696 nt) araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 68,258 A→G V27A (GTC→GCC)  araA ← L‑arabinose isomerase
RA 69,884 A→C R55R (CGT→CGG araB ← ribulokinase
RA 99,166 A→G E255G (GAA→GGA)  ftsW → putative lipid II flippase FtsW
RA 147,694 A→G T243A (ACG→GCG)  rpnC → recombination‑promoting nuclease RpnC
RA 169,469 T→C G662G (GGT→GGC fhuA → ferrichrome outer membrane transporter/phage receptor
RA 184,575 A→G G165G (GGT→GGC yaeI ← phosphodiesterase YaeI
RA 211,646 A→G V656V (GTA→GTG ldcC → lysine decarboxylase 2
RA 234,139 (A)7→8 coding (644/756 nt) gloB ← hydroxyacylglutathione hydrolase GloB
RA 256,057 A→G E27E (GAA→GAG gpt → xanthine‑guanine phsophoribosyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 260,012 (TTA)2→1 coding (87‑89/1056 nt) phoE ← outer membrane porin PhoE
RA 262,141 G→A S213S (TCG→TCA proA → glutamate‑5‑semialdehyde dehydrogenase
RA 406,413 A→G Q3Q (CAA→CAG aroL → shikimate kinase 2
RA 424,176 A→G V553V (GTA→GTG malZ → maltodextrin glucosidase
RA 424,314 T→C A599A (GCT→GCC malZ → maltodextrin glucosidase
RA 465,926 A→G L463L (TTA→CTA)  ybaE ← putative protein YbaE
RA 497,702 A→G A176A (GCA→GCG adk → adenylate kinase
RA 514,464 A→G G132G (GGT→GGC ybbJ ← NfeD‑like family protein
RA 519,496 A→G R90R (CGT→CGC tesA ← multifunctional acyl‑CoA thioesterase I and protease I and lysophospholipase L1
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 531,377 A→G V282A (GTA→GCA)  allS ← DNA‑binding transcriptional activator AllS
RA 548,466 A→G N37S (AAC→AGC)  ylbE → DUF1116 domain‑containing protein YlbE
RA 661,741 A→G S180P (TCA→CCA)  lipB ← lipoyl(octanoyl) transferase
RA 682,156 Δ1 bp coding (1238/1671 nt) hscC ← chaperone protein HscC
RA 684,717 A→G V180A (GTG→GCG)  gltL ← glutamate/aspartate ABC transporter ATP binding subunit
RA 699,393 (G)5→6 intergenic (‑216/+181) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 724,939 A→G L14P (CTG→CCG)  kdpC ← K(+) transporting P‑type ATPase subunit KdpC
RA 744,943 T→C V234A (GTG→GCG)  pxpC → 5‑oxoprolinase component C
RA 745,130 A→G Q296Q (CAA→CAG pxpC → 5‑oxoprolinase component C
RA 800,192 T→C E128E (GAA→GAG ybhD ← putative DNA‑binding transcriptional regulator YbhD
RA 804,652 A→G T384A (ACC→GCC)  ybhJ → putative hydratase YbhJ
RA 817,768 A→G Y242C (TAC→TGC)  moaA → GTP 3',8'‑cyclase
RA 846,166 A→G F100L (TTT→CTT)  glnQ ← L‑glutamine ABC transporter ATP binding subunit
RA 880,928 A→G T68A (ACT→GCT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 885,658 A→G I158I (ATT→ATC ybjI ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 964,293 A→G intergenic (+181/‑27) ihfB → / → ycaI integration host factor subunit beta/conserved inner membrane protein YcaI
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,035,913 A→G G48G (GGA→GGG hyaD → putative hydrogenase 1 maturation protease HyaD
RA 1,069,275 A→G F189L (TTT→CTT)  rutG ← pyrimidine:H(+) symporter
RA 1,080,039 (G)5→6 coding (735/1509 nt) putP → proline:Na(+) symporter
RA 1,087,870 A→G F663S (TTT→TCT)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
RA 1,093,358 (G)6→7 coding (483/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,125,779 G→C G73A (GGT→GCT)  rimJ → ribosomal‑protein‑S5‑alanine N‑acetyltransferase
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,172,289 (T)5→4 coding (1676/3447 nt) mfd ← transcription‑repair coupling factor
RA 1,176,499 T→C V358A (GTG→GCG)  lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,177,312 (G)5→4 coding (694/702 nt) lolD → lipoprotein release complex ‑ ATP binding subunit
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,230,039 A→G V119A (GTA→GCA)  hlyE ← hemolysin E
RA 1,285,716 T→C V193A (GTC→GCC)  narJ → nitrate reductase 1 molybdenum cofactor assembly chaperone
RA 1,297,536 G→A P196S (CCG→TCG)  adhE ← aldehyde‑alcohol dehydrogenase
RA 1,370,957 (A)6→5 coding (742/1680 nt) ycjM → glucosylglycerate phosphorylase
RA 1,390,048 A→G V184A (GTA→GCA)  mpaA ← murein tripeptide amidase A
RA 1,433,698 T→C intergenic (‑24/+293) pinR ← / ← ynaE Rac prophage; putative site‑specific recombinase/Rac prophage; uncharacterized protein YnaE
RA 1,437,231 (T)9→8 intergenic (+2/+29) micC → / ← ydbK small regulatory RNA MicC/putative pyruvate‑flavodoxin oxidoreductase
RA 1,480,639 (A)6→7 coding (1502/1758 nt) ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,493,440 T→C V324A (GTG→GCG)  trg → methyl‑accepting chemotaxis protein Trg
RA 1,494,533 A→G V180A (GTT→GCT)  ydcI ← putative DNA‑binding transcriptional repressor YdcI
RA 1,512,747 T→C V365A (GTT→GCT)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,570,471 T→C T7A (ACA→GCA)  gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,585,724 T→C T255A (ACC→GCC)  ydeP ← putative oxidoreductase YdeP
JC 1,594,132 (C)8→11 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,628,174 T→C S220P (TCA→CCA)  ydfG → 3‑hydroxy acid dehydrogenase
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,661,816 T→C F421L (TTC→CTC)  ynfF → putative selenate reductase YnfF
RA 1,666,880 T→C T114A (ACT→GCT)  ynfK ← putative dethiobiotin synthetase
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,685,614 C→T V325V (GTG→GTA fumC ← fumarase C
RA 1,692,622 A→G V77A (GTT→GCT)  uidC ← outer membrane porin family protein
RA 1,702,242 A→G T4A (ACC→GCC)  add → adenosine deaminase
RA 1,734,952 T→C V173A (GTT→GCT)  mepH → peptidoglycan DD‑endopeptidase MepH
RA 1,784,778 T→G T779P (ACC→CCC)  ppsA ← phosphoenolpyruvate synthetase
RA 1,810,689 T→C V160A (GTA→GCA)  ydjM → putative inner membrane protein regulated by LexA
RA 1,853,608 (C)5→4 coding (372/1359 nt) ydjE ← putative transporter YdjE
RA 1,862,532 Δ1 bp intergenic (‑103/‑239) msrB ← / → gapA methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,897,109 C→T H60Y (CAC→TAC)  sdaA → L‑serine deaminase I
RA 1,904,787 (T)7→8 intergenic (+41/‑14) manZ → / → yobD mannose‑specific PTS enzyme IID component/conserved inner membrane protein YobD
RA 1,913,058 T→C N587D (AAC→GAC)  prc ← tail‑specific protease
RA 1,914,439 A→G R126R (CGT→CGC prc ← tail‑specific protease
RA 1,974,541 T→C T262A (ACC→GCC)  cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,981,526 (C)5→4 coding (87/1425 nt) otsA ← trehalose‑6‑phosphate synthase
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,048,262 C→T H1109Y (CAT→TAT)  yeeJ → inverse autotransporter adhesin
RA 2,083,073 T→C R106R (CGT→CGC sbcB → exodeoxyribonuclease I
RA 2,095,988 T→C F49L (TTC→CTC)  hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,141,905 A→G V104A (GTG→GCG)  dcd ← dCTP deaminase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,196,870 T→C L133L (TTA→CTA)  yehF → putative protein YehF
RA 2,245,248 T→C T508A (ACG→GCG)  cirA ← ferric dihyroxybenzoylserine outer membrane transporter
RA 2,253,852 A→G G98G (GGT→GGC nupX ← putative nucleoside transporter
RA 2,322,934 T→C S357P (TCA→CCA)  atoC → DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC
RA 2,326,966 (C)6→7 coding (858/1185 nt) atoB → acetyl‑CoA acetyltransferase
RA 2,329,419 G→A Q127* (CAA→TAA)  yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,366,679 T→C V257A (GTC→GCC)  arnB → UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
RA 2,375,918 (C)5→6 coding (45/963 nt) menC ← o‑succinylbenzoate synthase
RA 2,416,523 A→G T593A (ACC→GCC)  pta → phosphate acetyltransferase
RA 2,417,748 T→C V223A (GTT→GCT)  yfcC → putative transporter YfcC
RA 2,419,382 (C)6→7 coding (407/555 nt) yfcE ← phosphodiesterase YfcE
RA 2,436,339 G→A P104L (CCC→CTC)  usg ← putative semialdehyde dehydrogenase Usg
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,450,815 A→G G87G (GGT→GGC yfcQ ← putative fimbrial protein YfcQ
RA 2,457,102 A→G G686G (GGT→GGC fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
RA 2,462,889 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,481,025 T→C L384L (TTA→TTG emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,545,766 A→G intergenic (‑157/‑7) murR ← / → murQ DNA‑binding transcriptional dual regulator MurR/N‑acetylmuramic acid 6‑phosphate etherase
RA 2,554,937 A→G G82G (GGT→GGC eutR ← putative AraC‑type transcriptional regulator EutR
RA 2,572,997 A→G V170A (GTG→GCG)  eutD ← phosphate acetyltransferase EutD
RA 2,579,480 Δ1 bp coding (815/951 nt) talA → transaldolase A
RA 2,592,874 A→G N38S (AAC→AGC)  ypfN → UPF0370 protein YpfN
RA 2,603,550 Δ1 bp coding (1733/2019 nt) hyfB → hydrogenase 4 component B
RA 2,604,643 T→C V266A (GTT→GCT)  hyfC → hydrogenase 4 component C
RA 2,669,247 T→C R72R (CGT→CGC hcaE → putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,682,157 (C)7→8 coding (589/3282 nt) yphG ← DUF5107 domain‑containing protein YphG
RA 2,710,619 T→C V67A (GTG→GCG)  nadB → L‑aspartate oxidase
RA 2,713,744 A→G V283V (GTA→GTG srmB → ATP‑dependent RNA helicase SrmB
RA 2,751,998 T→C F68F (TTT→TTC recN → DNA repair protein RecN
RA 2,755,958 Δ1 bp intergenic (+3/‑201) ssrA → / → intA tmRNA/CP4‑57 prophage; integrase
RA 2,771,767 T→C intergenic (+152/+73) yfjT → / ← yfjU CP4‑57 prophage; uncharacterized protein YfjT/CP4‑57 prophage; ArsC family protein YfjU
RA 2,779,615 T→C S1038G (AGT→GGT)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,795,309 T→C Y353H (TAT→CAT)  gabP → 4‑aminobutyrate:H(+) symporter
RA 2,800,962 T→C S80S (AGT→AGC nrdH → glutaredoxin‑like protein
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,826,581 Δ1 bp coding (190/960 nt) srlE → sorbitol‑specific PTS enzyme IIBC1 component
RA 2,838,210 (A)5→4 intergenic (‑105/+44) hydN ← / ← ascG putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG
RA 2,854,583 T→C G82G (GGT→GGC fhlA → DNA‑binding transcriptional activator FhlA
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,870,459 T→C E282E (GAA→GAG truD ← tRNA pseudouridine(13) synthase
RA 2,878,931 Δ1 bp coding (842/918 nt) cas1 ← multifunctional nuclease Cas1
RA 2,884,093 (C)6→7 coding (46/1509 nt) casA ← type I‑E CRISPR system Cascade subunit CasA
RA 2,909,780 C→A intergenic (‑114/+114) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,919,542 T→C D402G (GAC→GGC)  gudX ← glucarate dehydratase‑related protein
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,999,680 (G)7→8 coding (212/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,008,218 G→A A237T (GCC→ACC)  ygeX → 2,3‑diaminopropionate ammonia‑lyase
RA 3,014,470 (C)5→6 coding (588/771 nt) yqeC ← uncharacterized protein YqeC
RA 3,014,810 G→A P83L (CCC→CTC)  yqeC ← uncharacterized protein YqeC
RA 3,042,391 Δ1 bp intergenic (+98/+98) ygfZ → / ← yqfA folate‑binding protein/hemolysin‑III family protein
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,080,925 T→C K237K (AAA→AAG tktA ← transketolase 1
RA 3,084,513 T→C N467D (AAC→GAC)  speA ← arginine decarboxylase, biosynthetic
RA 3,110,512 (A)7→6 intergenic (+71/+78) pheV → / ← yghD tRNA‑Phe/putative type II secretion system M‑type protein
RA 3,121,491 T→G intergenic (‑212/+143) glcA ← / ← glcB glycolate/lactate:H(+) symporter GlcA/malate synthase G
RA 3,175,797 (C)6→7 coding (210/582 nt) yqiA ← esterase YqiA
RA 3,181,068 (A)5→4 coding (648/1161 nt) ygiC → putative enzyme with ATPase activity
RA 3,184,692 +G intergenic (‑226/‑148) ribB ← / → ubiK 3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase/ubiquinone biosynthesis accessory factor UbiK
RA 3,200,184 A→G V261A (GTC→GCC)  ygiF ← inorganic triphosphatase
RA 3,208,454 T→C V144A (GTG→GCG)  ttdT → L‑tartrate:succinate antiporter
RA 3,276,593 A→G T438A (ACC→GCC)  garD → galactarate dehydratase
RA 3,313,070 T→C T37A (ACC→GCC)  rbfA ← 30S ribosome binding factor
RA 3,313,824 T→C T731A (ACC→GCC)  infB ← translation initiation factor IF‑2
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,368,591 G→A R255R (CGG→CGA yhcG → DUF1016 domain‑containing protein YhcG
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,417,574 T→C S848P (TCA→CCA)  acrF → multidrug efflux pump RND permease AcrF
RA 3,467,351 A→G F835L (TTC→CTC)  chiA ← endochitinase
RA 3,490,412 A→G C140R (TGC→CGC)  pabA ← aminodeoxychorismate synthase subunit 2
RA 3,495,452 T→C V481A (GTT→GCT)  nirB → nitrite reductase catalytic subunit NirB
RA 3,521,143 T→C T103A (ACG→GCG)  hofO ← DNA utilization protein HofO
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,565,807 (T)5→4 coding (775/2448 nt) glgP ← glycogen phosphorylase
RA 3,577,633 T→C intergenic (‑41/+98) gntK ← / ← gntR D‑gluconate kinase, thermostable/DNA‑binding transcriptional repressor GntR
RA 3,590,214 A→G V233A (GTG→GCG)  ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,616,756 T→C L192P (CTG→CCG)  nikC → Ni(2(+)) ABC transporter membrane subunit NikC
RA 3,617,688 T→C V225A (GTA→GCA)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,633,571 A→G pseudogene (888/1607 nt) yhiL ← putative uncharacterized protein YhiL
RA 3,688,801 T→C T125A (ACC→GCC)  bcsC ← cellulose biosynthesis protein BcsC
RA 3,717,091 A→G D182G (GAC→GGC)  yiaD → PF13488 family lipoprotein YiaD
RA 3,743,478 T→C V249A (GTC→GCC)  yiaK → 2,3‑diketo‑L‑gulonate reductase
RA 3,781,358 +G coding (144/474 nt) trmL → tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,798,725 A→G D196D (GAT→GAC waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
RA 3,799,764 T→A I145F (ATC→TTC)  waaZ ← lipopolysaccharide core biosynthesis protein WaaZ
RA 3,801,399 A→G L201L (TTG→CTG)  waaJ ← UDP‑glucose:(glucosyl)LPS alpha‑1,2‑glucosyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,820,592 (C)6→7 coding (579/1683 nt) ligB ← DNA ligase B
RA 3,852,713 T→C E24E (GAA→GAG ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,875,245 A→G intergenic (‑85/‑193) dgoR ← / → yidX putative DNA‑binding transcriptional regulator DgoR/putative lipoprotein YidX
RA 3,878,829 A→G S431P (TCT→CCT)  gyrB ← DNA gyrase subunit B
RA 3,918,383 T→C Y492C (TAC→TGC)  atpA ← ATP synthase F1 complex subunit alpha
RA 3,924,789 T→C D282G (GAC→GGC)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,958,274 T→C V102A (GTG→GCG)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,970,199 T→C L23L (TTG→CTG)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase
RA 4,027,240 (A)7→6 intergenic (+12/+369) fre → / ← fadA flavin reductase/3‑ketoacyl‑CoA thiolase
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,066,547 A→G R216R (CGT→CGC yihP ← putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
RA 4,084,051 T→C N591S (AAC→AGC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,090,672 C→T G85D (GGC→GAC)  frvX ← peptidase M42 family protein
RA 4,095,814 (C)6→7 coding (169/1260 nt) rhaA ← L‑rhamnose isomerase
RA 4,155,920 A→G D307G (GAC→GGC)  argC → N‑acetylglutamylphosphate reductase
RA 4,228,038 A→G D103G (GAC→GGC)  yjbB → putative inorganic phosphate export protein
RA 4,244,166 A→G C304R (TGT→CGT)  malF ← maltose ABC transporter membrane subunit MalF
RA 4,272,877 A→G V332A (GTC→GCC)  uvrA ← excision nuclease subunit A
RA 4,285,800 A→G G524G (GGT→GGC acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,292,161 +G coding (650/1659 nt) nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,293,152 (G)5→6 coding (1641/1659 nt) nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,324,212 T→C K47K (AAA→AAG phnD ← phosphonate ABC transporter periplasmic binding protein
RA 4,327,860 A→G E242E (GAA→GAG crfC → clamp‑binding sister replication fork colocalization protein
RA 4,363,063 A→G R82R (CGT→CGC yjdC ← putative DNA‑binding transcriptional regulator YjdC
RA 4,364,243 A→G L267P (CTC→CCC)  dsbD ← protein disulfide oxidoreductase ‑ DsbDreduced
RA 4,432,376 (G)5→6 coding (681/966 nt) ytfF ← inner membrane protein YtfF
RA 4,437,277 A→G T175A (ACC→GCC)  cysQ → 3'(2'),5'‑bisphosphate nucleotidase
RA 4,442,363 (A)6→5 intergenic (‑187/‑19) msrA ← / → tamA methionine sulfoxide reductase A/translocation and assembly module subunit TamA
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,461,803 A→G G286G (GGT→GGC nrdD ← anaerobic ribonucleoside‑triphosphate reductase
RA 4,482,624 A→G V405A (GTA→GCA)  valS ← valine‑‑tRNA ligase
RA 4,497,997 A→G intergenic (+57/‑275) intB → / → insC‑6 KpLE2 phage‑like element; putative integrase/KpLE2 phage‑like element; IS2 insertion element repressor InsA
RA 4,512,668 G→A L248F (CTT→TTT)  fecC ← ferric citrate ABC transporter membrane subunit FecC
RA 4,533,126 A→G F20L (TTC→CTC)  yjhP ← KpLE2 phage‑like element; putative methyltransferase YjhP
RA 4,582,333 T→C D239G (GAC→GGC)  hsdM ← type I restriction enzyme EcoKI methyltransferase component
RA 4,589,575 A→G F569L (TTC→CTC)  btsT ← pyruvate:H(+) symporter
RA 4,601,884 Δ1 bp coding (214/474 nt) yjjB ← putative succinate exporter YjjB
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→7 noncoding (72/87 nt) leuQ ← tRNA‑Leu
MC JC 4,631,762 Δ12 bp coding (1030‑1041/1938 nt) slt → soluble lytic murein transglycosylase
RA 4,637,384 A→G D230G (GAT→GGT)  creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423766–3424234 3424523–3424239 6–758 18 [17] [15] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)15 (0.330) 11/188 2.5 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375023 =NA (NA)13 (0.280) 11/190 2.5 NA intergenic (+142/‑436) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375118NA (NA)16 (0.310) 13/214 2.5 NA noncoding (1/36 nt) other REP31d
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1207790 =2 (0.030)71 (1.220) 50/242 0.1 97.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 2 (0.030)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078052 (0.030)37 (0.630) 29/242 0.9 95.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 2 (0.030)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)81 (1.260) 53/266 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =95 (1.440)93 (1.420) 61/272 0.1 65.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)89 (1.350) 62/274 0.1 92.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.110)coding (3907/4134 nt) rhsA rhs element protein RhsA