Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
252 |
48.5 |
1661298 |
98.8% |
1641362 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,308,976 |
A→G |
intergenic (‑52/+57) |
nlpI ← / ← pnp |
lipoprotein NlpI/polynucleotide phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,308,976 | 0 | A | G | 100.0%
| 66.3
/ NA
| 21 | intergenic (‑52/+57) | nlpI/pnp | lipoprotein NlpI/polynucleotide phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/6); total (15/6) |
TTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAA‑G‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCT > NC_000913/3308841‑3309107
|
ttCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAg > 2:421891/1‑140 (MQ=255)
aCTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCg < 1:776723/140‑1 (MQ=255)
tACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTg > 2:164621/1‑140 (MQ=255)
aCGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGc < 1:421891/140‑1 (MQ=255)
ggAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGtt > 1:818827/1‑140 (MQ=255)
aGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTaaa < 1:540960/140‑1 (MQ=255)
tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑G‑cccccccgcc > 2:635318/1‑136 (MQ=255)
tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCa > 1:777322/1‑140 (MQ=255)
ttAAGGGCTGGCGAAACAAAACCCCGGCGCAAAAAAGGCTTCTTTCCCCACTCCCGACGCCCACGGTAGAATGAACGGCCTTTTCCGGGTTGCGCGCATGGCGTCCTGCGCGGTAAAAG‑GCCCCCCCGCCGCAGCGGAgg < 2:777322/139‑1 (MQ=255)
aaGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTCCCCACCCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCGGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGc < 2:570416/140‑1 (MQ=255)
cccAGCGAAAAAAAGGCTTCATTTCCCCCTCCCGAAGCCCACGGTTGAATGAACGTCCTGTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc < 1:297470/139‑1 (MQ=255)
acCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGcc > 2:520699/1‑140 (MQ=255)
cgcAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGtt > 1:21513/1‑140 (MQ=255)
aGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTCCCCGGTTGCTAGCAGGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGcc < 2:439388/140‑1 (MQ=255)
cTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGa > 2:376193/1‑140 (MQ=255)
tCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgctg > 1:72767/1‑140 (MQ=255)
cgaaccccACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAGGGCGTCCTGCCCGGTTAAAC‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag < 2:508586/134‑1 (MQ=255)
caaaGCCCACGGTTAAAGAACCGTCCTTTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAACC‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag < 2:21513/138‑1 (MQ=255)
cGAAGCCCACGGTTGAATGACCGTCCTGTCCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag < 2:156192/140‑1 (MQ=255)
cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag < 2:298021/133‑1 (MQ=255)
cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcag > 1:298021/1‑133 (MQ=255)
cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTg > 1:167496/1‑140 (MQ=255)
aCGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga < 1:376193/140‑1 (MQ=255)
cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgag > 1:123322/1‑140 (MQ=255)
cGGTTGAATGAACGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTgag > 1:286691/1‑140 (MQ=255)
aatgaaCGTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCt < 1:124281/140‑1 (MQ=255)
gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGTTAAAAACC‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc < 2:167496/140‑1 (MQ=255)
gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc > 2:288762/1‑140 (MQ=255)
gTCCTGTTCCCGGTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc < 2:123322/140‑1 (MQ=255)
gTTGCTAGCAAGGCGTCCTGCCCGGTTAAAA‑GCCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCt > 2:279421/1‑140 (MQ=255)
|
TTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAA‑G‑CCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCT > NC_000913/3308841‑3309107
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
