Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,068	C→T	R152H (CGT→CAT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,068	0	C	T	100.0%	178.2 / NA	50	R152H (CGT→CAT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/28); total (22/28)

GGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACA  >  NC_000913/4629932‑4630203

ggATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTa                                                                                                                                      >  1:436655/1‑140 (MQ=255)

     cGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCa                                                                                                                                 >  2:259672/1‑140 (MQ=255)

         aTCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATccg                                                                                                                             <  2:1055083/140‑1 (MQ=255)

         aTCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATccg                                                                                                                             <  1:259672/140‑1 (MQ=255)

              ggtggtTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCa                                                                                                                        <  2:261362/140‑1 (MQ=255)

                 ggttggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     <  1:358133/140‑1 (MQ=255)

                  gttggtCGGTTCGTCGAGCAGGAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCt                                                                                                                    >  2:805661/1‑140 (MQ=255)

                    tggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTcc                                                                                                                  <  1:79271/140‑1 (MQ=255)

                     ggtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCa                                                                                                                 >  1:738593/1‑140 (MQ=255)

                      gtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     <  1:930348/135‑1 (MQ=255)

                      gtCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGc                                                                                                                     >  2:930348/1‑135 (MQ=255)

                       tCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGc                                                                                                               >  2:465113/1‑140 (MQ=255)

                             cgtcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACg                                                                                                         >  1:433333/1‑140 (MQ=255)

                               tcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGtt                                                                                                       <  2:84732/140‑1 (MQ=255)

                               tcgAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGtt                                                                                                       <  2:910353/140‑1 (MQ=255)

                                      gcagcaTGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTa                                                                                                <  1:348923/140‑1 (MQ=255)

                                          caTGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGAc                                                                                            <  1:1128904/140‑1 (MQ=255)

                                            tGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCAGCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcg                                                                                          >  1:871877/1‑140 (MQ=255)

                                               cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcg                                                                                       <  2:196000/140‑1 (MQ=255)

                                               cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcg                                                                                       <  2:173180/140‑1 (MQ=255)

                                               cTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGAACGTTCAGATTATGACcgtcg                                                                                       <  2:494765/140‑1 (MQ=255)

                                                tGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCAGCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACcgtcgt                                                                                      <  2:871877/140‑1 (MQ=255)

                                                    ttttCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  <  1:805661/140‑1 (MQ=255)

                                                     tttCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGcc                                                                                 >  2:646933/1‑140 (MQ=255)

                                                        ccAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  <  1:134779/136‑1 (MQ=255)

                                                        ccAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGc                                                                                  >  2:134779/1‑136 (MQ=255)

                                                         cagcagcagGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGaa                                                                             <  2:482855/140‑1 (MQ=255)

                                                             agcagGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGAt                                                                         <  1:465113/140‑1 (MQ=255)

                                                                 ggcggcACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATctct                                                                     >  1:108513/1‑140 (MQ=255)

                                                                       acaACGCTACGCGACGCCGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGa                                                               <  2:1018574/140‑1 (MQ=255)

                                                                       acaACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGa                                                               <  2:108513/140‑1 (MQ=255)

                                                                                cgcgACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGt                                                      >  1:205479/1‑140 (MQ=255)

                                                                                     cgacgTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGc                                                 >  2:458677/1‑140 (MQ=255)

                                                                                      gacgTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCg                                                >  1:210709/1‑140 (MQ=255)

                                                                                            caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGc                                          <  1:458677/140‑1 (MQ=255)

                                                                                            caccacCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGc                                          <  2:433333/140‑1 (MQ=255)

                                                                                                  cGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCa                                    >  2:56988/1‑140 (MQ=255)

                                                                                                      gagGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAAt                                <  2:499191/140‑1 (MQ=255)

                                                                                                       agGTTAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATc                               >  1:802429/1‑140 (MQ=255)

                                                                                                           tAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCa                           >  2:675840/1‑140 (MQ=255)

                                                                                                           tAGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCa                           >  1:128585/1‑140 (MQ=255)

                                                                                                            aGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCAt                          >  2:196519/1‑140 (MQ=255)

                                                                                                            aGCGATTTTCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCAt                          >  2:143475/1‑140 (MQ=255)

                                                                                                                    tCGCGTCCCAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCg                  >  2:165246/1‑140 (MQ=255)

                                                                                                                          cccAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTAc            <  1:165246/140‑1 (MQ=255)

                                                                                                                           ccAGTCCGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACa           <  2:210709/140‑1 (MQ=255)

                                                                                                                                ccGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCa      <  2:205479/140‑1 (MQ=255)

                                                                                                                                ccGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCa      <  1:675840/140‑1 (MQ=255)

                                                                                                                                 cGGCAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCAt     <  1:196519/140‑1 (MQ=255)

                                                                                                                                    cAGATGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATaca  >  1:960379/1‑140 (MQ=255)

GGATTCGGCATCCAGGTGGTTGGTCGGTTCGTCGAGCAGCAGCATGTCTGGTTTTTCCAGCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACA  >  NC_000913/4629932‑4630203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A