Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
176 |
31.5 |
1082462 |
98.8% |
1069472 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,338,233 |
T→C |
T77A (ACC→GCC) |
mlaD ← |
intermembrane phospholipid transport system, substrate binding protein MlaD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,338,233 | 0 | T | C | 100.0%
| 86.9
/ NA
| 25 | T77A (ACC→GCC) | mlaD | intermembrane phospholipid transport system, substrate binding protein MlaD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/12); total (13/12) |
TCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTA > NC_000913/3338097‑3338361
|
tCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAACGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGcttt < 2:430161/140‑1 (MQ=255)
aTGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCAcc < 1:462001/140‑1 (MQ=255)
ccAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGa < 1:220649/140‑1 (MQ=255)
atatTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCca > 2:432918/1‑140 (MQ=255)
ttCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCAcaacaa > 2:395422/1‑140 (MQ=255)
ccccAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGc < 1:39985/140‑1 (MQ=255)
ggCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATa > 1:73730/1‑140 (MQ=255)
ccGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACt < 1:395422/140‑1 (MQ=255)
cTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAgcgcgc > 1:193347/1‑140 (MQ=255)
aGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCttt > 2:446789/1‑140 (MQ=255)
ggTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGgccgc > 2:407618/1‑140 (MQ=255)
tgtgGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCg > 2:318095/1‑140 (MQ=255)
tgttCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAg > 1:64399/1‑140 (MQ=255)
gttCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCAt < 1:132018/135‑1 (MQ=255)
gttCAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCAt > 2:132018/1‑135 (MQ=255)
cAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCgtg < 2:73730/140‑1 (MQ=255)
cAATTTCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCgtg < 1:155873/140‑1 (MQ=255)
tttCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGCCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAg > 2:285961/1‑140 (MQ=255)
tCCAGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGt < 1:407618/140‑1 (MQ=255)
aGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCgtg < 1:500535/132‑1 (MQ=255)
aGCGTTACGCGCGGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCgtg > 2:500535/1‑132 (MQ=255)
cgcgcgGCAGATAGGCTTTCGTGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTa > 2:167248/1‑140 (MQ=255)
cgcgcgGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGt > 2:208842/1‑139 (MQ=255)
cgcgcgGCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGt < 1:208842/139‑1 (MQ=255)
gggCAGATAGGCTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTa < 1:285961/139‑1 (MQ=255)
cgGCAGATAGGCTTTCGTGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTa < 1:167248/140‑1 (MQ=255)
|
TCCGGGTCTTCAAAACCGACGTTTAATGCCAGATATTGTTCCCCCAGCAGGCCGGAAGTACGAATGCTCAGCGAACTGGTATCTGGAATGTGGTTATAACGTTGTTCAATTTCCAGCGTTACGCGCGGCAGATAGGTTTTCGGGTCCAGCGTAATATCCGCCACCCGACCCACAACAACGCCACCAATACTGACCGGAGAGCGCGCTTTCAGGCCGCCAATGTTATCGAACGTCGCATAAAGCGTGTAGGTCGGTTCAGTACGTA > NC_000913/3338097‑3338361
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
