Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A31 F30 I1 R1	28	44.1	1499574	99.3%	1489076	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,030,852	G→T	G40G (GGC→GGA)	fadB ←	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,030,852	0	G	T	100.0%	137.9 / NA	40	G40G (GGC→GGA)	fadB	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/21); total (19/21)

TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCC  >  NC_000913/4030719‑4030988

ttAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAgg                                                                                                                                    >  1:51191/1‑140 (MQ=255)

      gTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCg                                                                                                                              >  2:494039/1‑140 (MQ=255)

        tcttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGAc                                                                                                                            >  1:491795/1‑140 (MQ=255)

               gAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCa                                                                                                                     <  1:55386/140‑1 (MQ=255)

                 aCGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgt                                                                                                                   >  1:198571/1‑140 (MQ=255)

                  cGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgtg                                                                                                                  <  1:646652/140‑1 (MQ=255)

                   gAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgtgt                                                                                                                 <  2:598889/140‑1 (MQ=255)

                    aGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTc                                                                                                                >  1:703687/1‑140 (MQ=255)

                     ggAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCg                                                                                                               <  1:368001/140‑1 (MQ=255)

                         cAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGtt                                                                                                           >  2:192897/1‑140 (MQ=255)

                          aGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGttt                                                                                                          <  2:570992/140‑1 (MQ=255)

                            ggACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTttat                                                                                                        <  2:47937/140‑1 (MQ=255)

                                aaaaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAAc                                                                                                    >  1:406516/1‑140 (MQ=255)

                                 aaaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACt                                                                                                   <  2:498373/140‑1 (MQ=255)

                                    aTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGaa                                                                                                >  1:553814/1‑140 (MQ=255)

                                          tGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTggg                                                                                          >  1:402433/1‑140 (MQ=255)

                                          tGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTggg                                                                                          <  2:1704/140‑1 (MQ=255)

                                           gATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTgggg                                                                                         <  1:129461/140‑1 (MQ=255)

                                             tatCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCa                                                                                       <  1:443879/140‑1 (MQ=255)

                                              atCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCAt                                                                                      >  1:410284/1‑140 (MQ=255)

                                                        acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAGCCTGGGGCATCAAATACCAg                                                                            >  2:1501/1‑140 (MQ=255)

                                                        acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAg                                                                            >  1:557471/1‑140 (MQ=255)

                                                        acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAg                                                                            >  1:274553/1‑140 (MQ=255)

                                                         cgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGt                                                                           <  2:553814/140‑1 (MQ=255)

                                                             aaaGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCgg                                                                       <  1:569952/140‑1 (MQ=255)

                                                             aaaGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCgg                                                                       <  2:733448/140‑1 (MQ=255)

                                                                  ctgctTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATg                                                                  <  2:39467/140‑1 (MQ=255)

                                                                    gctTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGcc                                                                <  2:59196/140‑1 (MQ=255)

                                                                               aCGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTccagcc                                                     <  1:192897/140‑1 (MQ=255)

                                                                                 gcagcagcaGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATCCCAGTTCGGCAATGCCATCTTccagccag                                                   <  2:23959/140‑1 (MQ=255)

                                                                                    gcagcagcCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCa                                                <  1:258844/140‑1 (MQ=255)

                                                                                             cTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAgg                                       >  2:109751/1‑140 (MQ=255)

                                                                                                    aTCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGc                                <  1:494039/140‑1 (MQ=255)

                                                                                                      cTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCt                              >  1:80350/1‑140 (MQ=255)

                                                                                                       tGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACATGGTGTCGCCtt                             <  1:473362/140‑1 (MQ=255)

                                                                                                         aTTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTg                           >  2:736925/1‑140 (MQ=255)

                                                                                                           tGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTa                         <  2:410284/140‑1 (MQ=255)

                                                                                                                ttCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCa                    >  1:713586/1‑140 (MQ=255)

                                                                                                                ttCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCa                    >  2:649214/1‑140 (MQ=255)

                                                                                                                          ccGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGtctc          >  1:595717/1‑140 (MQ=255)

                                                                                                                                  ctctCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGCGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATcc  <  2:703687/140‑1 (MQ=255)

TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCC  >  NC_000913/4030719‑4030988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A