Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F30 I1 R1	21	20.2	686622	99.1%	680442	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,654,245	C→A	L95L (CTC→CTA)	slp →	starvation lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,654,245	0	C	A	100.0%	96.8 / NA	29	L95L (CTC→CTA)	slp	starvation lipoprotein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (13/16); total (13/16)

GTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTCGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAGGG  >  NC_000913/3654109‑3654379

gTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGcc                                                                                                                                     >  1:295399/1‑140 (MQ=255)

   aacaaGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGa                                                                                                                                  >  2:12632/1‑140 (MQ=255)

           cgcTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATA‑TGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGc                                                                                                                         <  1:118901/140‑1 (MQ=255)

                            tATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGa                                                                                                         >  2:198933/1‑140 (MQ=255)

                                        cAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATc                                                                                             <  1:61766/140‑1 (MQ=255)

                                         aaTGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCa                                                                                            <  1:12632/140‑1 (MQ=255)

                                           tGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACt                                                                                          <  2:2238/140‑1 (MQ=255)

                                              cAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGaa                                                                                                        <  2:335942/123‑1 (MQ=255)

                                              cAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGaa                                                                                                        >  1:335942/1‑123 (MQ=255)

                                                      aTACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACtt                                                                                         <  2:145993/130‑1 (MQ=255)

                                                      aTACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACtt                                                                                         >  1:145993/1‑130 (MQ=255)

                                                            tgttAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGc                                                                         <  2:326653/140‑1 (MQ=255)

                                                                  aaaTCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGATAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   <  2:295399/140‑1 (MQ=255)

                                                                    aTCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCa                                                                 >  2:53249/1‑140 (MQ=255)

                                                                         tGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTg                                                            <  1:279732/140‑1 (MQ=255)

                                                                           tATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaa                                                          <  1:311587/140‑1 (MQ=255)

                                                                               aCCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   >  1:251563/1‑127 (MQ=255)

                                                                               aCCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCAtt                                                                   <  2:251563/127‑1 (MQ=255)

                                                                                   ttGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATACAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaacaac                                                      <  2:18968/136‑1 (MQ=255)

                                                                                   ttGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATACAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGaacaac                                                      >  1:18968/1‑136 (MQ=255)

                                                                                    tGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGc                                                 <  2:305592/140‑1 (MQ=255)

                                                                                     ggATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCt                                                >  2:329486/1‑140 (MQ=255)

                                                                                                     ccTGATATTGAAGCCAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTccc                                >  2:212239/1‑140 (MQ=255)

                                                                                                                   cAACTATCAGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTgg                  <  2:67868/140‑1 (MQ=255)

                                                                                                                           aGGGCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAat          >  1:36391/1‑140 (MQ=255)

                                                                                                                              gCCGACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATg       <  2:251544/140‑1 (MQ=255)

                                                                                                                                 gACTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAg    <  1:60160/140‑1 (MQ=255)

                                                                                                                                  aCTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAgg   >  2:37900/1‑140 (MQ=255)

                                                                                                                                   cTGCTAGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAggg  >  1:269667/1‑140 (MQ=255)

GTCAACAAGCGCGCTTTGGTGGGAAGGTTATCAACGTTATCAATGGCAAAACGGATACGTTGTTAGAAATCTCTGTATTACCGTTGGATAGCTATGCGAAGCCTGATATTGAAGCCAACTATCAGGGCCGACTGCTCGCCAGACAAAGCGGCTTCCTTGATCCAGTGAACTATCGTAATCACTTTGTTACCATCCTCGGCACCATTCAGGGTGAACAACCTGGCTTTATCAATAAAGTCCCGTATAACTTCCTGGAAGTGAATATGCAGGG  >  NC_000913/3654109‑3654379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A