Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A29 F30 I1 R1 26 45.9 1554102 99.2% 1541669 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,682,266 C→A A394A (GCG→GCT)  dctA ← C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,682,2660CA100.0% 209.7 / NA 59A394A (GCG→GCT) dctAC4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/28);  total (31/28)

GGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAC  >  NC_000913/3682130‑3682402
                                                                                                                                        |                                                                                                                                        
ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:9682/1‑140 (MQ=255)
ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:675005/1‑140 (MQ=255)
ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:300702/1‑140 (MQ=255)
      gggAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCg                                                                                                                                 >  2:257186/1‑140 (MQ=255)
          gTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTAc                                                                                                                             >  2:351199/1‑140 (MQ=255)
             cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:610431/140‑1 (MQ=255)
             cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:701713/140‑1 (MQ=255)
             cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:300702/140‑1 (MQ=255)
             cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  2:251617/140‑1 (MQ=255)
             cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:351199/140‑1 (MQ=255)
              gggCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGAc                                                                                                                         >  2:765965/1‑140 (MQ=255)
                  aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTa                                                                                                                              <  1:326552/131‑1 (MQ=255)
                  aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTa                                                                                                                              >  2:326552/1‑131 (MQ=255)
                  aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAgg                                                                                                                     >  2:325330/1‑140 (MQ=255)
                       tgatgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGt                                                                                                                <  1:601347/140‑1 (MQ=255)
                          tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          >  2:599639/1‑127 (MQ=255)
                          tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:599639/127‑1 (MQ=255)
                          tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAg                                                                                                             >  2:124068/1‑140 (MQ=255)
                            agaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAgcg                                                                                                           <  2:337261/140‑1 (MQ=255)
                              aTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCa                                                                                                         <  1:9682/140‑1 (MQ=255)
                                tAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACg                                                                                                       <  1:257186/140‑1 (MQ=255)
                                     ggATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  2:752693/140‑1 (MQ=255)
                                     ggATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  2:511066/140‑1 (MQ=255)
                                      gATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTc                                                                                                 >  1:270132/1‑140 (MQ=255)
                                      gATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTc                                                                                                 <  2:211403/140‑1 (MQ=255)
                                        tAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATAGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  1:520405/137‑1 (MQ=255)
                                        tAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATAGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  >  2:520405/1‑137 (MQ=255)
                                               tGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        >  1:200742/1‑140 (MQ=255)
                                                 cGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        <  2:8939/138‑1 (MQ=255)
                                                 cGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        >  1:8939/1‑138 (MQ=255)
                                                     ttGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTc                                                                                  <  1:325330/140‑1 (MQ=255)
                                                       gCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGa                                                                                <  1:124068/140‑1 (MQ=255)
                                                                cgcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGa                                                                       >  1:164833/1‑140 (MQ=255)
                                                                               cacaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCg                                                        >  2:421388/1‑140 (MQ=255)
                                                                                acaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGc                                                       <  2:200742/140‑1 (MQ=255)
                                                                                 caTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                            >  1:402156/1‑134 (MQ=255)
                                                                                 caTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                            <  2:402156/134‑1 (MQ=255)
                                                                                  aTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTa                                                     <  2:110812/140‑1 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                          >  1:11768/1‑133 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                          <  2:11768/133‑1 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  1:466336/1‑140 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  2:650105/1‑140 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  2:577834/1‑140 (MQ=255)
                                                                                    cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  1:760512/1‑140 (MQ=255)
                                                                                               ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGcc                                        <  2:164833/140‑1 (MQ=255)
                                                                                               ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGcc                                        <  2:270132/140‑1 (MQ=255)
                                                                                                               tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:708388/1‑140 (MQ=255)
                                                                                                               tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:701561/1‑140 (MQ=255)
                                                                                                               tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:124925/1‑140 (MQ=255)
                                                                                                                  cccACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgcc                     <  2:124925/140‑1 (MQ=255)
                                                                                                                         aGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCa              >  1:550835/1‑140 (MQ=255)
                                                                                                                               gACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaa        <  2:760512/140‑1 (MQ=255)
                                                                                                                                aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:411351/1‑140 (MQ=255)
                                                                                                                                aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:392764/1‑140 (MQ=255)
                                                                                                                                aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:51249/1‑140 (MQ=255)
                                                                                                                                aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  1:506760/1‑140 (MQ=255)
                                                                                                                                 cAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAg      >  2:707757/1‑140 (MQ=255)
                                                                                                                                  aaTGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGc     <  1:577834/140‑1 (MQ=255)
                                                                                                                                     ggTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAc  <  1:675005/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAC  >  NC_000913/3682130‑3682402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A