Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F30 I1 R1	22	86.0	2916610	99.3%	2896193	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,895	G→A	G392G (GGC→GGT)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,895	0	G	A	100.0%	162.5 / NA	47	G392G (GGC→GGT)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/24); total (23/24)

CGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTT  >  NC_000913/2632762‑2633025

cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              >  1:1044127/1‑140 (MQ=255)

cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:383852/140‑1 (MQ=255)

cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:423443/140‑1 (MQ=255)

cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:829663/140‑1 (MQ=255)

cgccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:1328424/140‑1 (MQ=255)

 gccCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             <  2:967933/140‑1 (MQ=255)

    cATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAAc                                                                                                                          <  2:52736/140‑1 (MQ=255)

          ctgGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATAc                                                                                                                    <  2:453398/140‑1 (MQ=255)

           tgGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATAcc                                                                                                                   <  2:36632/140‑1 (MQ=255)

               gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             <  2:651552/126‑1 (MQ=255)

               gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACgg                                                                                                               <  2:630946/140‑1 (MQ=255)

               gAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGAAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAggg                                                                                                                             >  1:651552/1‑126 (MQ=255)

                  tGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              <  2:751726/122‑1 (MQ=255)

                  tGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAgg                                                                                                                              >  1:751726/1‑122 (MQ=255)

                       tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTaa                                                                                                            <  2:845017/135‑1 (MQ=255)

                       tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTaa                                                                                                            >  1:845017/1‑135 (MQ=255)

                       tctTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGAttt                                                                                                       >  1:147359/1‑140 (MQ=255)

                          ttCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTa                                                                                                    >  2:1201881/1‑140 (MQ=255)

                               gCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:534864/140‑1 (MQ=255)

                               gCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:1044127/140‑1 (MQ=255)

                                 gACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACgg                                                                                             <  1:1201881/140‑1 (MQ=255)

                                   ccTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGcc                                                                                           <  2:1362857/140‑1 (MQ=255)

                                   ccTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGcc                                                                                           >  1:1094123/1‑140 (MQ=255)

                                             cTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGt                                                                                 >  1:1131607/1‑140 (MQ=255)

                                                cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGTACCCAGGAAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  2:748242/1‑140 (MQ=255)

                                                cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               <  2:855110/123‑1 (MQ=255)

                                                cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAAc                                                                                               >  1:855110/1‑123 (MQ=255)

                                                cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  1:1348477/1‑140 (MQ=255)

                                                cgcgACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCg                                                                              >  1:673590/1‑140 (MQ=255)

                                                      cTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCa                                                                        >  1:731008/1‑140 (MQ=255)

                                                         cGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCAccc                                                                     <  2:1366122/140‑1 (MQ=255)

                                                              ccTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGgaga                                                                <  2:1426025/140‑1 (MQ=255)

                                                                  ccGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGAttct                                                            >  1:1455530/1‑140 (MQ=255)

                                                                   cGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGAttctt                                                           <  2:8528/140‑1 (MQ=255)

                                                                    ggCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGCAGAGTTCGATTTCACCCGGAGAttcttc                                                          >  2:739666/1‑140 (MQ=255)

                                                                      cACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAg                                                        >  1:924330/1‑140 (MQ=255)

                                                                             ttGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGc                                                 <  2:1348477/140‑1 (MQ=255)

                                                                                  ggCAGCGTAATCGCTCTGGAAAAAACGTACAAAGGAACCTTTGGACATCGCACCAAGGGAACCCATCCCCCGGTAAGATTTGTAACAACGGCCCTGTTAGAGTTCGATTTCCCCCGGAGATTTTTCAGTCCCCGCCAGCa                                            <  2:1131607/140‑1 (MQ=255)

                                                                                      gCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGTTTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGa                                        <  2:1094123/140‑1 (MQ=255)

                                                                                       cGTTATCGCTCTGGAAATAACGGTCAGAGGATCCTTTGGACATCGCACCCAGGGAACCCATACCGCGGTAAGATTTGTAAGAACGGCCCTGGTAGATTTCGATTTCCCCGGGAGATTCTTCAGTACCCGCCAGCATGGaa                                       <  2:147359/140‑1 (MQ=255)

                                                                                                    gAAATAACGGTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACcg                          >  2:931272/1‑140 (MQ=255)

                                                                                                      aaaatcGGTGAGAGTACCCTTTGGCCATCGCCCCGAGGGAACCCATACCAGGATAAGATTTGTAAGAACGGCCCTGAAAGATTTGTTTTCCACCCGGAGTTTCTTCGGGACCCGCCAGCATGGAACCTACCATCACcgcg                        <  2:349594/135‑1 (MQ=255)

                                                                                                            ggTCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTgcg                  >  2:35660/1‑140 (MQ=255)

                                                                                                              tCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGcc                >  2:484928/1‑140 (MQ=255)

                                                                                                              tCAGAGGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGcc                >  2:249943/1‑140 (MQ=255)

                                                                                                               cAGAGAACCCTTTGGACACCGCACCAAGGAACCCCATCCCACGTTAAGATTTGTAAGAACGGCCCGGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCa               <  2:731008/140‑1 (MQ=255)

                                                                                                                agagGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAg              <  1:788037/140‑1 (MQ=255)

                                                                                                                agagGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAg              >  1:349594/1‑140 (MQ=255)

                                                                                                                  agGAACCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAgcg            >  1:91426/1‑140 (MQ=255)

                                                                                                                      cccTTTGGACATCGCCCCCAGGGACCCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGTTTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGa        <  2:673590/139‑1 (MQ=255)

                                                                                                                      aCCTTTGGACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGa        >  1:740657/1‑140 (MQ=255)

                                                                                                                            ggACATCGCACCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCtt  >  1:777902/1‑140 (MQ=255)

CGCCCATCTGCTGGTGAATGATCTCTTTCAGGCGACCTTTATAGGCTACGCGACCTTCGATACCTTCCGGCACCAGTTTGTCGGCAGCGTTATCGCTCTGGAAATAACGGTCAGAGGAACCTTTGGACATCGCGCCCAGGGAACCCATACCACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTT  >  NC_000913/2632762‑2633025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A