Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F73 I1 R1 29 140.0 4785556 99.3% 4752057 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,608,061 C→A S537R (AGC→AGA)  zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,0610CA97.3% 255.3 / ‑3.1 74S537R (AGC→AGA) zntAZn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (26/46);  total (27/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCACCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGATC  >  NC_000913/3607927‑3608193
                                                                                                                                      |                                                                                                                                    
cccACCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCgg                                                                                                                                 <  2:1944453/140‑1 (MQ=255)
  cACCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGc                                                                                                                               <  1:887400/140‑1 (MQ=255)
   aCCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCa                                                                                                                              <  1:137506/140‑1 (MQ=255)
    ccgccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCaa                                                                                                                             >  2:423837/1‑140 (MQ=255)
    ccgccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCaa                                                                                                                             >  2:270550/1‑140 (MQ=255)
     cgccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCaaa                                                                                                                            <  2:2197163/140‑1 (MQ=255)
       ccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACg                                                                                                                          <  1:683918/140‑1 (MQ=255)
        cgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACgg                                                                                                                         >  1:1751219/1‑140 (MQ=255)
         gAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGt                                                                                                                        <  2:1442161/140‑1 (MQ=255)
         gAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGt                                                                                                                        <  2:1009899/140‑1 (MQ=255)
          aaTCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTa                                                                                                                       >  1:2171448/1‑140 (MQ=255)
          aaTCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTa                                                                                                                       <  1:88959/140‑1 (MQ=255)
            tCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGt                                                                                                                     <  1:1705306/140‑1 (MQ=255)
             cacaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTg                                                                                                                    <  2:1787163/140‑1 (MQ=255)
              acaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGc                                                                                                                   <  1:1490493/140‑1 (MQ=255)
                 gCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTgg                                                                                                                <  2:2327589/140‑1 (MQ=255)
                 gCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTgg                                                                                                                <  2:114331/140‑1 (MQ=255)
                        cTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACg                                                                                                         <  2:1338856/140‑1 (MQ=255)
                           gTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTaa                                                                                                      <  1:270550/140‑1 (MQ=255)
                               ggTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGAt                                                                                                  >  1:367258/1‑140 (MQ=255)
                                 tCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGa                                                                                                <  2:1378807/140‑1 (MQ=255)
                                  cTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGAc                                                                                               <  2:870491/140‑1 (MQ=255)
                                   tGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACg                                                                                              <  1:943338/140‑1 (MQ=255)
                                          gAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCt                                                                                          >  2:2359182/1‑137 (MQ=255)
                                          gAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCt                                                                                          <  1:2359182/137‑1 (MQ=255)
                                          gAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTgg                                                                                       >  2:1625061/1‑140 (MQ=255)
                                             gcgcAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGgtgt                                                                                    <  1:249355/140‑1 (MQ=255)
                                               gcAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCa                                                                                  <  2:477513/140‑1 (MQ=255)
                                               gcAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCa                                                                                  >  2:967824/1‑140 (MQ=255)
                                                  ggTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                               <  1:2283483/140‑1 (MQ=255)
                                                  ggTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                               <  1:1016462/140‑1 (MQ=255)
                                                    ttAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCg                                                                             <  1:2159664/140‑1 (MQ=255)
                                                    ttAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGG‑TTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGt                                                                            <  2:29052/140‑1 (MQ=255)
                                                    ttAACGGTGAGCGCGTATTCATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCg                                                                             <  1:1967008/140‑1 (MQ=255)
                                                      aaCGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                           <  2:63009/140‑1 (MQ=255)
                                                      aaCGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                           <  1:320005/140‑1 (MQ=255)
                                                        cGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTAc                                                                         <  2:1493797/140‑1 (MQ=255)
                                                             aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                           >  1:118495/1‑133 (MQ=255)
                                                             aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                           <  2:118495/133‑1 (MQ=255)
                                                             aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                    >  2:1919494/1‑140 (MQ=255)
                                                             aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                    >  2:2331792/1‑140 (MQ=255)
                                                             aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                    >  2:314768/1‑140 (MQ=255)
                                                               cgcgTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                               >  2:1590138/1‑127 (MQ=255)
                                                               cgcgTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                               <  1:1590138/127‑1 (MQ=255)
                                                                                tGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGAtgctg                                                 <  2:2171448/140‑1 (MQ=255)
                                                                                   cGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCaa                                              <  1:1919494/140‑1 (MQ=255)
                                                                                       aaaCATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGc                                          <  2:1751219/140‑1 (MQ=255)
                                                                                       aaaCATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGc                                          <  1:423837/140‑1 (MQ=255)
                                                                                               cGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATc                                      <  1:2232037/136‑1 (MQ=255)
                                                                                               cGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATc                                      >  2:2232037/1‑136 (MQ=255)
                                                                                               cGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGtg                                  >  2:2284711/1‑140 (MQ=255)
                                                                                                    aTGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGtgaactg                             >  2:1166258/1‑140 (MQ=255)
                                                                                                          ttACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAAcgcg                       <  2:1882442/140‑1 (MQ=255)
                                                                                                           tACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACgcgc                      <  1:1625061/140‑1 (MQ=255)
                                                                                                           tACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACgcgc                      <  2:367258/140‑1 (MQ=255)
                                                                                                            aCTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCt                     <  1:2284711/140‑1 (MQ=255)
                                                                                                              tGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTgg                   >  1:1417370/1‑140 (MQ=255)
                                                                                                               ggTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTggg                  >  2:643552/1‑140 (MQ=255)
                                                                                                               ggTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTggg                  >  2:422252/1‑140 (MQ=255)
                                                                                                                gTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGc                 >  1:1428287/1‑140 (MQ=255)
                                                                                                                gTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGc                 >  1:1052805/1‑140 (MQ=255)
                                                                                                                gTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGc                 >  1:2037135/1‑140 (MQ=255)
                                                                                                                   taattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTc              >  1:1042048/1‑140 (MQ=255)
                                                                                                                    aattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCa             >  2:1568271/1‑140 (MQ=255)
                                                                                                                    aattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCa             <  1:25478/140‑1 (MQ=255)
                                                                                                                     attaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCaa            <  1:552068/140‑1 (MQ=255)
                                                                                                                     attaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCaa            <  1:1166258/140‑1 (MQ=255)
                                                                                                                          cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAgggg       >  2:2351561/1‑140 (MQ=255)
                                                                                                                          cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAgggg       >  1:2210568/1‑140 (MQ=255)
                                                                                                                          cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAgggg       <  1:1568271/140‑1 (MQ=255)
                                                                                                                          cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAgggg       >  1:1490098/1‑140 (MQ=255)
                                                                                                                            aaCTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTg     <  2:1841106/140‑1 (MQ=255)
                                                                                                                            aaCTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTg     <  1:314768/140‑1 (MQ=255)
                                                                                                                               tGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGATc  >  1:927323/1‑140 (MQ=255)
                                                                                                                               tGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGATc  <  1:32417/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                    
CCCACCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGATC  >  NC_000913/3607927‑3608193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A