Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F73 I1 R1
|
26 |
44.8 |
1518598 |
99.2% |
1506449 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,629,295 |
G→T |
R82S (CGC→AGC) |
rbbA ← |
ribosome‑associated ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,629,295 | 0 | G | T | 100.0%
| 78.3
/ NA
| 23 | R82S (CGC→AGC) | rbbA | ribosome‑associated ATPase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/12); total (11/12) |
CCCGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCGATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGC > NC_000913/3629166‑3629430
|
cccGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCggg < 1:102258/140‑1 (MQ=255)
cccGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCggg > 2:274286/1‑140 (MQ=255)
ccgctccgctTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGt < 1:519243/140‑1 (MQ=255)
ttGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATc > 1:442044/1‑140 (MQ=255)
gACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGccc < 2:442044/140‑1 (MQ=255)
aCAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGATGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCAcc > 2:20743/1‑140 (MQ=255)
aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc < 2:400962/140‑1 (MQ=255)
aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc < 1:409982/140‑1 (MQ=255)
aaaaaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGcc < 1:274286/140‑1 (MQ=255)
aaaTCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTg > 2:55913/1‑140 (MQ=255)
aCGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAAt < 2:429305/140‑1 (MQ=255)
ccGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCg > 2:310415/1‑140 (MQ=255)
aCAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGaa > 1:694896/1‑140 (MQ=255)
aGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGaca < 1:520543/124‑1 (MQ=255)
aGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGaca > 2:520543/1‑124 (MQ=255)
cTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGc > 2:354774/1‑140 (MQ=255)
aTCCAGGCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCgg > 2:302584/1‑140 (MQ=255)
gCGATGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAAt < 1:310415/140‑1 (MQ=255)
aTGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAg < 2:694896/140‑1 (MQ=255)
tGCGCGGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGc > 1:165879/1‑140 (MQ=255)
cgcgGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGccc < 2:165879/140‑1 (MQ=255)
cgcgGGCAGACGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGccc > 2:132037/1‑140 (MQ=255)
cGTCGCGGCTATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAg < 1:354774/140‑1 (MQ=255)
gcggctATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCggg < 2:247288/140‑1 (MQ=255)
cggctATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGc > 2:36796/1‑140 (MQ=255)
|
CCCGCTCCGCTTTGTCGTGACCGAACAGGCGAGCGAAAAAATCGACGTTTTCATACACCGACAAGGTGTGGTAGAGGTTTTTGCCCAGCCCCTGCGGCATCCAGGCGATGCGCGGGCAGACGTCGCGGCGATGCTTCGGGTCGCGCATATCGCCGCCCAGCACCATCACATTGCCCTGTTCAATGACGCGGGCACCGGAAATCAACGACAACAAGCTCGACTTCCCGACGCCGTCCGGGCCAATCAGCCCGACCATACAGCGGGC > NC_000913/3629166‑3629430
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
