Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,689,231	T→G	D344A (GAT→GCT)	bcsZ ←	endo‑1,4‑D‑glucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,689,231	0	T	G	100.0%	95.5 / NA	27	D344A (GAT→GCT)	bcsZ	endo‑1,4‑D‑glucanase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (15/12); total (15/12)

CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAAC  >  NC_000913/3689095‑3689366

cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGccc                                                                                                                                      >  2:693023/1‑140 (MQ=255)

cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGccc                                                                                                                                      >  2:149271/1‑140 (MQ=255)

              tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAAc                                                                                                                        <  1:12350/140‑1 (MQ=255)

                gACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAg                                                                                                                      <  2:388651/140‑1 (MQ=255)

                 aCCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAgg                                                                                                                     >  1:13975/1‑140 (MQ=255)

                 aCCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAgg                                                                                                                     >  2:246692/1‑140 (MQ=255)

                        gCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGca                                                                                                              <  1:387822/140‑1 (MQ=255)

                        gCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGca                                                                                                              <  2:538968/140‑1 (MQ=255)

                          aTGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGcaca                                                                                                            <  1:633279/140‑1 (MQ=255)

                           tGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACAt                                                                                                           <  1:142585/140‑1 (MQ=255)

                             aCGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAg                                                                                                         <  1:161679/140‑1 (MQ=255)

                                                 cGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGcc                                                                                     <  1:149271/140‑1 (MQ=255)

                                                               gtgtGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCgg                                                                       >  1:250951/1‑140 (MQ=255)

                                                                  tgAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCa                                                                    >  1:299795/1‑140 (MQ=255)

                                                                       ttGCGCATTCCTGGCCCCAGTCAGGTAATAGCTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACgcgc                                                               <  2:330631/140‑1 (MQ=255)

                                                                          cgcATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACgcgctgg                                                            >  1:344430/1‑140 (MQ=255)

                                                                              ttCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACgcgctggcgct                                                        >  2:368341/1‑140 (MQ=255)

                                                                                ccTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGTCACGCGCTGGCGCTGa                                                      <  2:418381/140‑1 (MQ=255)

                                                                                         aGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTgc                                             >  1:695388/1‑140 (MQ=255)

                                                                                           tCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTgcgc                                           >  2:354261/1‑140 (MQ=255)

                                                                                                         aCCTTTTGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTa                             >  2:618903/1‑140 (MQ=255)

                                                                                                             tttGTCGAGAAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTaaaaa                         >  2:569624/1‑140 (MQ=255)

                                                                                                                     gaAGCGGAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGgcagca                 >  2:527434/1‑140 (MQ=255)

                                                                                                                           gAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATggcgg           <  2:13975/140‑1 (MQ=255)

                                                                                                                           gAAACGGTGTTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATggcgg           >  1:507569/1‑140 (MQ=255)

                                                                                                                                   gtTGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGaaaaa   <  1:415675/140‑1 (MQ=255)

                                                                                                                                    ttGAGCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAAc  >  2:368226/1‑140 (MQ=255)

CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCTTGTCCAAACAGGGTCAGCACATAGTTGTAATAGGCATCGCTGCCGGGAAAGTTATCGGCCACGCGCTGGCGCTGAACGGCCTGCGCATCGCGGTTTTGTAAAAAGGGCAGCATGGCGGCAGAAAAAC  >  NC_000913/3689095‑3689366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A