Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,425,329	G→T	V196V (GTC→GTA)	hisQ ←	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,425,329	0	G	T	100.0%	175.0 / NA	49	V196V (GTC→GTA)	hisQ	lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (32/17); total (32/17)

CGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAGTTGTTGCCAATGCCTGG  >  NC_000913/2425201‑2425463

cGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGaa                                                                                                                             >  2:185938/1‑140 (MQ=255)

cGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGaa                                                                                                                             >  1:583138/1‑140 (MQ=255)

     tCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCTCACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGa                                                                                                                        >  2:234709/1‑140 (MQ=255)

      ccAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGaa                                                                                                                       <  2:78524/140‑1 (MQ=255)

           aTTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGtt                                                                                                                  <  1:839027/140‑1 (MQ=255)

             tCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTcc                                                                                                                >  1:104960/1‑140 (MQ=255)

                tGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAg                                                                                                             <  1:462776/140‑1 (MQ=255)

                    aGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTAc                                                                                                         >  2:529254/1‑140 (MQ=255)

                    aGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTAc                                                                                                         >  2:762541/1‑140 (MQ=255)

                        ttCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACtttt                                                                                                     >  2:658984/1‑140 (MQ=255)

                                caGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCa                                                                                             >  1:726479/1‑140 (MQ=255)

                                 aGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAg                                                                                            <  1:607143/140‑1 (MQ=255)

                                      aGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTggg                                                                                       <  2:726479/140‑1 (MQ=255)

                                           tctTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCt                                                                                  <  2:893938/140‑1 (MQ=255)

                                             ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCCGTTGGGTGGCttt                                                                                >  2:297462/1‑140 (MQ=255)

                                             ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCttt                                                                                >  1:355800/1‑140 (MQ=255)

                                               cacaCCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGa                                                                              >  2:308778/1‑140 (MQ=255)

                                                 cacCCACGGAGTAGCGGCTCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTGCTTTTGCCTGCCAGTTGGGTGGCTTTGAcc                                                                            <  2:32417/140‑1 (MQ=255)

                                                  acCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACca                                                                           >  1:818873/1‑140 (MQ=255)

                                                   cccACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACcac                                                                          <  1:407940/140‑1 (MQ=255)

                                                   cccACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACcac                                                                          <  2:835777/140‑1 (MQ=255)

                                                    ccACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACcaca                                                                         >  1:581387/1‑140 (MQ=255)

                                                     cACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACAt                                                                        >  1:307319/1‑140 (MQ=255)

                                                       cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCt                                                                      >  2:678697/1‑140 (MQ=255)

                                                       cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCt                                                                      >  1:308755/1‑140 (MQ=255)

                                                       cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCt                                                                      >  1:57848/1‑140 (MQ=255)

                                                       cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCt                                                                      >  2:525190/1‑140 (MQ=255)

                                                       cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCt                                                                      >  1:50243/1‑140 (MQ=255)

                                                            tAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAg                                                                 <  2:567543/140‑1 (MQ=255)

                                                               cggcgCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGcc                                                              >  1:656522/1‑140 (MQ=255)

                                                                 gcgcTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGa                                                            >  1:83808/1‑140 (MQ=255)

                                                                    cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTa                                                         >  1:521892/1‑140 (MQ=255)

                                                                    cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTa                                                         >  1:508553/1‑140 (MQ=255)

                                                                      cAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAAc                                                       >  2:431282/1‑140 (MQ=255)

                                                                           aaCAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAAc                                                  <  1:185938/140‑1 (MQ=255)

                                                                                   cacaCCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCgg                                          <  2:104960/140‑1 (MQ=255)

                                                                                    acacCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGt                                         <  2:521892/140‑1 (MQ=255)

                                                                                        cATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAAc                                                       >  2:92007/1‑122 (MQ=255)

                                                                                        cATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAAc                                                       <  1:92007/122‑1 (MQ=255)

                                                                                          ttGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGAttt                                   >  1:807430/1‑140 (MQ=255)

                                                                                          ttGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGAttt                                   >  1:631736/1‑140 (MQ=255)

                                                                                                  aGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGTGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCa                           <  2:893058/140‑1 (MQ=255)

                                                                                                  aGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCa                           >  2:581104/1‑140 (MQ=255)

                                                                                                  aGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCa                           >  2:243040/1‑140 (MQ=255)

                                                                                                  aGTGGTGAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCa                           >  1:61538/1‑140 (MQ=255)

                                                                                                    tggtgAAAACCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCAcc                         >  1:656576/1‑140 (MQ=255)

                                                                                                            aCCAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAgtt                 >  1:525053/1‑140 (MQ=255)

                                                                                                             ccAGGTAAATCACGCCACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAgttg                <  2:581387/140‑1 (MQ=255)

                                                                                                                 gTAATTCACGCCACATACGATGGCGAAATAGAAGGGTGCCCAGGTACTTTTGCCTGCCAGTAGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAGTTGTTGc            <  2:779187/140‑1 (MQ=255)

                                                                                                                           ccACATACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAGTTGTTGCCAATGCCTgg  <  1:234709/140‑1 (MQ=255)

CGGTTTCCAGTATTCATGTAAGATTTCGATCACAGGTCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCCTGCCAGTTGGGTGGCTTTGACCACATCTTCCAGGCCGAGTAACGAAACCAGTGCGGTAGATTTGAGGATCACCTGCCAGTTGTTGCCAATGCCTGG  >  NC_000913/2425201‑2425463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A