Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,547,948	C→A	P426T (CCG→ACG)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,547,948	0	C	A	100.0%	115.7 / NA	34	P426T (CCG→ACG)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/13); total (21/13)

CCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGCCGTAACG  >  NC_000913/2547813‑2548083

ccGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACgtc                                                                                                                                      <  1:205130/140‑1 (MQ=255)

                tCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGGCTGCCGATGGGGCTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGGGCTg                                                                                                                      >  2:212159/1‑140 (MQ=255)

                   cccTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCg                                                                                                                   <  2:239363/140‑1 (MQ=255)

                        ccccGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCtgat                                                                                                              >  1:791836/1‑140 (MQ=255)

                        ccccGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCtgat                                                                                                              <  1:343280/140‑1 (MQ=255)

                        ccccGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCtgat                                                                                                              >  2:6562/1‑140 (MQ=255)

                        ccccGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAACGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCtgat                                                                                                              >  2:144763/1‑140 (MQ=255)

                          ccGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCtgatga                                                                                                            >  2:303854/1‑140 (MQ=255)

                             cATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACtt                                                                                                         >  2:593477/1‑140 (MQ=255)

                                  aGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGca                                                                                                    <  1:261112/140‑1 (MQ=255)

                                  aGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGca                                                                                                    >  1:454484/1‑140 (MQ=255)

                                     cGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACaa                                                                                                 >  2:529980/1‑140 (MQ=255)

                                         tGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCa                                                                                             <  1:416830/140‑1 (MQ=255)

                                         tGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCa                                                                                             <  2:706019/140‑1 (MQ=255)

                                                 ccTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCg                                                                                     >  1:598159/1‑140 (MQ=255)

                                                   tGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGc                                                                                   >  1:358257/1‑140 (MQ=255)

                                                     tttAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCa                                                                                 >  1:272500/1‑140 (MQ=255)

                                                     tttAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCa                                                                                 >  1:873820/1‑140 (MQ=255)

                                                       tAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATg                                                                               <  1:529980/140‑1 (MQ=255)

                                                       tAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATg                                                                               <  2:598159/140‑1 (MQ=255)

                                                                              tttATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCt                                                        <  2:173956/140‑1 (MQ=255)

                                                                                               cTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCg                                      >  2:518711/1‑140 (MQ=255)

                                                                                                      ggTCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTAt                               <  1:212159/140‑1 (MQ=255)

                                                                                                        tCTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGGTTAttt                             >  1:756600/1‑140 (MQ=255)

                                                                                                         cTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGGTTCT‑GGGGGCATGGGGTTGCGGGGTTAtttt                            >  2:750002/1‑140 (MQ=255)

                                                                                                         cTGCCGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGACTTT‑GGCGGCCGGGGGGTGGGGGGTTAtttt                            >  2:137241/1‑140 (MQ=255)

                                                                                                            ccGATGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGGTTCT‑GGGGGCATGGGGTTGCGGGTTTATTTTtac                         >  2:115735/1‑140 (MQ=255)

                                                                                                               aTGGGCTTAAACAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGGTTCC‑GGTAGCATGGGGTTGCGGGGTTATTTTtaccac                      >  2:879011/1‑140 (MQ=255)

                                                                                                                         aCAGCGCCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGc            <  1:6562/140‑1 (MQ=255)

                                                                                                                             cgcCTTTTGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATGCTGGGTGGCTTGGG‑TTGCGGGTTTTTTTTCTCCACGCTTTTTGGCTGcc        >  2:233772/1‑140 (MQ=255)

                                                                                                                             cgcCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGcc        >  1:307939/1‑140 (MQ=255)

                                                                                                                             cgcCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGcc        >  1:13321/1‑140 (MQ=255)

                                                                                                                             cgcCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGGTTGCGGGTTTATTTTTACCACGCTCTTTGGCTGcc        >  2:305086/1‑140 (MQ=255)

                                                                                                                             cgcCTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGGTTGCGGGGTTATTTTTACCACGCTCTTTGGCTGcc        >  2:540107/1‑140 (MQ=255)

                                                                                                                               ccTTTGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGCCGt      <  1:303854/140‑1 (MQ=255)

                                                                                                                                   tGGGACGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGCCGTAACg  <  2:13321/140‑1 (MQ=255)

CCGCTGATTTACGGTGTCACCCTGCCCCGCATGAAGCCGTTTGTTACCGCCTGTTTAGGCGGCGCGGCGGGCGGTTTGTTTATCGGCCTGATAGCCTGGTGGGGTCTGCCGATGGGCTTAAACAGCGCCTTTGGGCCGTCTGGTCTGGTAGCGCTGCCGCTGATGACTTCCGCACAAGGCATCCTTCCGGCCATGGCGGTTTATGCTGGCGGGATTCT‑GGTGGCATGGGTTTGCGGGTTTATTTTCACCACGCTCTTTGGCTGCCGTAACG  >  NC_000913/2547813‑2548083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A