Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F73 I1 R1 29 53.4 1802238 99.2% 1787820 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,611,955 C→A A19E (GCG→GAG)  hyfR → DNA‑binding transcriptional activator HyfR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,611,9550CA100.0% 99.4 / NA 29A19E (GCG→GAG) hyfRDNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/9);  total (20/9)

GAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTA  >  NC_000913/2611832‑2612090
                                                                                                                           |                                                                                                                                       
gAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTc                                                                                                                         >  1:265561/1‑140 (MQ=255)
          tctatctGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGGAGAGGTAAACGGAATGCTCGCGGGGCGGt                                                                                                               >  1:178680/1‑140 (MQ=255)
           ttatcgGAGGGCCGAAAAATTTGGGGGAGAAGGTTTTCTCATAAATAAGGATTGTTGATGGCTATGGCGGAGGGGGCGATGTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGtt                                                                                                              <  2:130018/139‑1 (MQ=255)
               ctGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGca                                                                                                          >  1:362666/1‑140 (MQ=255)
                     gCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGaaa                                                                                                    >  2:753737/1‑140 (MQ=255)
                     gCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGaaa                                                                                                    >  1:738894/1‑140 (MQ=255)
                      ccAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAac                                                                                                   >  2:869845/1‑140 (MQ=255)
                      ccAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAac                                                                                                   >  2:730987/1‑140 (MQ=255)
                                     gagaGGGTTTTTTAATAAAAAGGGATTGTTGATGGTTATGTCAGAGGGGGCGTTGTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCtt                                                                                    <  2:873105/140‑1 (MQ=255)
                                            ttttCTCATAAATAAGGACTGTTGATGGTTATGTCAGACGGGGCGTTTTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTAcg                                                                             <  1:114984/139‑1 (MQ=255)
                                            ttttCTAATAAAAAAGGATTGTTGATGGCTATGTCAGAGGGGGGGTTTTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTAcg                                                                             <  1:806031/139‑1 (MQ=255)
                                                          aGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGc                                                               >  1:658977/1‑140 (MQ=255)
                                                               tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  1:408820/1‑140 (MQ=255)
                                                               tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  1:350182/1‑140 (MQ=255)
                                                               tGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccg                                                          >  2:25059/1‑140 (MQ=255)
                                                                  tGATGGTTATGAAAGAGGGGGCGTTTTTCCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTgccgccg                                                       <  2:52279/140‑1 (MQ=255)
                                                                      ggCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGt                                                   >  2:785073/1‑140 (MQ=255)
                                                                           tGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGc                                              >  2:746843/1‑140 (MQ=255)
                                                                           tGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGc                                              >  2:639122/1‑140 (MQ=255)
                                                                            gTCAGAGGGGGCTTTTTTTCCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGcc                                             <  1:661643/140‑1 (MQ=255)
                                                                            gTCAGACGGGGGGTTTTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGcc                                             <  1:443785/140‑1 (MQ=255)
                                                                             tCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGAACCGGGTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTCCAGccc                                            >  2:229250/1‑140 (MQ=255)
                                                                              cAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCg                                           >  1:384014/1‑140 (MQ=255)
                                                                                 aCGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTa                                        >  1:82605/1‑140 (MQ=255)
                                                                                  cGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGGAc                                       >  1:843732/1‑140 (MQ=255)
                                                                                     ggCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAAc                                    >  1:24904/1‑140 (MQ=255)
                                                                                                    gCCACAAGGAATAACAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATg                     <  2:362666/140‑1 (MQ=255)
                                                                                                                  cAATTGAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAAc       <  2:820473/140‑1 (MQ=255)
                                                                                                                       gAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTa  >  2:550892/1‑140 (MQ=255)
                                                                                                                       gAAGAGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCCCAAAAGCAACTTCTa  >  1:351678/1‑140 (MQ=255)
                                                                                                                           |                                                                                                                                       
GAACCGGCCATCTATCTGATGGCCAGAAAAATTGCGTGAGAAGGATTTCTCATTAATAAGGACTGTTGATGGCTATGTCAGACGAGGCGATGTTTGCCCCGCCACAAGGAATAACAATTGAAGCGGTAAACGGAATGCTCGCGGAGCGGTTAGCACAGAAACACGGCAAGGCGTCTTTATTACGCGCCTTCATCCCGCTGCCGCCGCCGTTCAGCCCGGTACAACTTATTGAACTGCATGTTCTCAAAAGCAACTTCTA  >  NC_000913/2611832‑2612090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A