Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,232,125	C→A	A154E (GCA→GAA)	fadH →	2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,232,125	0	C	A	100.0%	155.8 / NA	44	A154E (GCA→GAA)	fadH	2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (19/25); total (19/25)

ACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAG  >  NC_000913/3231993‑3232259

aCCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATac                                                                                                                                 >  1:584762/1‑140 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGTGGGAGGAAGGATacga                                                                                                                               <  1:300209/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:832550/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:584762/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:280431/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:150982/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  2:131223/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:737992/140‑1 (MQ=255)

  cAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacga                                                                                                                               <  1:579798/140‑1 (MQ=255)

   aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGCGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacgac                                                                                                                              >  2:732978/1‑140 (MQ=255)

   aaCCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATacgac                                                                                                                              >  2:18480/1‑140 (MQ=255)

               tCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTg                                                                                                                  >  1:584179/1‑140 (MQ=255)

                   cccGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATgg                                                                                                              <  2:586141/140‑1 (MQ=255)

                   cccGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATgg                                                                                                              >  1:574381/1‑140 (MQ=255)

                        ccGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTcc                                                                                                         >  2:896661/1‑140 (MQ=255)

                          gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGa                                                                                                       <  2:557763/140‑1 (MQ=255)

                          gCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCGGCAACTGATCGACAATTTCGCCCGCTGCGCGGAACTGGCGCGGGAGGAAGGATGCGACGGTGTAGAGGTGATGGGTTCCGa                                                                                                       <  2:479427/140‑1 (MQ=255)

                           cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:623446/140‑1 (MQ=255)

                           cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  2:503246/140‑1 (MQ=255)

                           cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  2:58210/140‑1 (MQ=255)

                           cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:18888/140‑1 (MQ=255)

                           cATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGaa                                                                                                      <  1:895569/140‑1 (MQ=255)

                                cAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTa                                                                                                 >  2:415701/1‑140 (MQ=255)

                                        cATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCa                                                                                         >  1:510784/1‑140 (MQ=255)

                                          tCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAAc                                                                                       >  1:132615/1‑140 (MQ=255)

                                             aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGaa                                                                                    >  1:666137/1‑140 (MQ=255)

                                                 tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                >  2:505870/1‑140 (MQ=255)

                                                      tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                           <  2:311102/140‑1 (MQ=255)

                                                      tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                           >  2:850958/1‑140 (MQ=255)

                                                            aTGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTgcgc                                                                     >  1:478775/1‑140 (MQ=255)

                                                             tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                    <  1:269278/140‑1 (MQ=255)

                                                                  tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                               <  2:666137/140‑1 (MQ=255)

                                                                    aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:584179/140‑1 (MQ=255)

                                                                    aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:478775/140‑1 (MQ=255)

                                                                    aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                             <  2:455327/140‑1 (MQ=255)

                                                                         tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                        >  2:276730/1‑140 (MQ=255)

                                                                              agaTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                                                   <  1:115096/140‑1 (MQ=255)

                                                                                aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                 >  1:523698/1‑140 (MQ=255)

                                                                                        aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg                                         >  1:486714/1‑140 (MQ=255)

                                                                                            aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                     >  2:70380/1‑140 (MQ=255)

                                                                                                             cTGCGCGCAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGT‑GGGCGGCGATTACCGCAACCGGATGCg                   >  2:221871/1‑140 (MQ=255)

                                                                                                                    cAACTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc             <  1:18480/140‑1 (MQ=255)

                                                                                                                      aCTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg           <  2:574381/140‑1 (MQ=255)

                                                                                                                      aCTGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg           <  1:732978/140‑1 (MQ=255)

                                                                                                                        tGGCGCGGGAGGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTa         >  2:395495/1‑140 (MQ=255)

                                                                                                                               ggaggaAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag  >  1:5244/1‑140 (MQ=255)

ACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAG  >  NC_000913/3231993‑3232259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A