Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F73 I1 R1 29 53.4 1802238 99.2% 1787820 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,314,889 C→A A376S (GCG→TCG)  infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,314,8890CA100.0% 162.5 / NA 47A376S (GCG→TCG) infBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

CGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGCCTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCGCTTTGAT  >  NC_000913/3314752‑3315018
                                                                                                                                         |                                                                                                                                 
cgcTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGT‑CAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGActc                                                                                                                                >  2:375327/1‑140 (MQ=255)
  cTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGActct                                                                                                                               >  1:280601/1‑140 (MQ=255)
               cACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGtttt                                                                                                                  >  1:165842/1‑140 (MQ=255)
                    tCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCacga                                                                                                             <  2:410387/140‑1 (MQ=255)
                     cGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCacgac                                                                                                            >  2:427602/1‑140 (MQ=255)
                      gTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCacgacg                                                                                                           <  2:280601/140‑1 (MQ=255)
                      gTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACAGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCacgacg                                                                                                           <  2:545521/140‑1 (MQ=255)
                       ttGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGGTGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGc                                                                                                          >  1:32857/1‑140 (MQ=255)
                         gAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAg                                                                                                        >  1:808980/1‑140 (MQ=255)
                            cGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGAt                                                                                                     <  2:520329/140‑1 (MQ=255)
                             gAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATa                                                                                                    <  2:213708/140‑1 (MQ=255)
                                    gTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTAt                                                                                             >  2:323138/1‑140 (MQ=255)
                                      tcagcagAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATgg                                                                                           <  1:375327/139‑1 (MQ=255)
                                          cagAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCa                                                                                       >  2:590110/1‑140 (MQ=255)
                                           agagagGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCAt                                                                                      <  1:49890/140‑1 (MQ=255)
                                             agagGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCAtct                                                                                    >  2:576106/1‑140 (MQ=255)
                                             agagGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCAtct                                                                                    >  1:365540/1‑140 (MQ=255)
                                                           ggTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGc                                                                      >  2:551764/1‑140 (MQ=255)
                                                               aaCGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCtgtg                                                                  >  2:476477/1‑140 (MQ=255)
                                                                    gACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGtt                                                             <  2:182690/140‑1 (MQ=255)
                                                                      cccATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTc                                                           >  1:232971/1‑140 (MQ=255)
                                                                        cATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCt                                                         <  1:692041/140‑1 (MQ=255)
                                                                         atgatgGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTg                                                        <  1:576106/140‑1 (MQ=255)
                                                                           gatgGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAGCTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGAt                                                      <  2:643814/140‑1 (MQ=255)
                                                                           gatgGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGAt                                                      >  1:506539/1‑140 (MQ=255)
                                                                           gatgGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGAt                                                      >  2:200461/1‑140 (MQ=255)
                                                                            atgGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATc                                                     <  2:808980/140‑1 (MQ=255)
                                                                               gTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGAt                                                  <  1:590110/140‑1 (MQ=255)
                                                                                tCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATa                                                 <  2:32857/140‑1 (MQ=255)
                                                                                  acaACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAAc                                               <  1:7604/140‑1 (MQ=255)
                                                                                        ggCGCGTGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGtt                                         <  2:666920/140‑1 (MQ=255)
                                                                                         gcgcgcgcGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTg                                        <  1:494648/140‑1 (MQ=255)
                                                                                          cgcgcgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGa                                       >  2:885692/1‑140 (MQ=255)
                                                                                          cgcgcgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGa                                       >  1:78501/1‑140 (MQ=255)
                                                                                          cgcgcgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGa                                       >  1:436674/1‑140 (MQ=255)
                                                                                           gcgcgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGAt                                      <  2:429492/140‑1 (MQ=255)
                                                                                              cgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGt                                   >  1:65630/1‑140 (MQ=255)
                                                                                              cgcgGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGt                                   >  2:664319/1‑140 (MQ=255)
                                                                                                   ttCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCa                              >  1:12270/1‑140 (MQ=255)
                                                                                                        ccGCAGCACCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTgcg                         <  2:585379/140‑1 (MQ=255)
                                                                                                           cagcaCCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGccc                      <  1:805669/140‑1 (MQ=255)
                                                                                                            agcaCCCGTGTCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCa                     >  1:197027/1‑140 (MQ=255)
                                                                                                                     gtCACGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTtcatca            <  1:509278/140‑1 (MQ=255)
                                                                                                                        aCGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCg         <  2:232971/140‑1 (MQ=255)
                                                                                                                        aCGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCg         >  1:384899/1‑140 (MQ=255)
                                                                                                                        aCGGTCGCTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCg         <  1:265349/140‑1 (MQ=255)
                                                                                                                               cTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTcatcat           <  2:135279/131‑1 (MQ=255)
                                                                                                                               cTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTcatcat           >  1:135279/1‑131 (MQ=255)
                                                                                                                               cTCATTACCGACTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCGCTTtgat  >  1:527951/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                 
CGCTTCGCCAGAGGCCACTTTCGTTGAACGAATGTAGTCCAGCAGAGAGGTTTTACCGTGGTCAACGTGACCCATGATGGTCACAACCGGCGCGCGCGGTTCAGCCGCAGCACCCGTGTCACGGTCGCTCATTACCGCCTCTTCCAGCTCGTTTTCACGACGCAGGATAACTTTATGGCCCATCTCTTCAGCAACCAGCTGTGCGGTTTCCTGATCGATAACCTGGTTGATGGTTGCCATTGCGCCCAGTTTCATCATCGCTTTGAT  >  NC_000913/3314752‑3315018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A