Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F73 I1 R1 31 34.8 1203930 99.1% 1193094 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,335,704 G→T A264E (GCG→GAG)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,335,7040GT100.0% 95.6 / NA 28A264E (GCG→GAG) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/11);  total (17/11)

GGGCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCG  >  NC_000913/3335570‑3335829
                                                                                                                                      |                                                                                                                             
gggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGc                                                                                                                          >  2:124725/1‑140 (MQ=255)
 ggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCa                                                                                                                         >  1:388452/1‑140 (MQ=255)
 ggCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCa                                                                                                                         <  2:124549/140‑1 (MQ=255)
   ccTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACg                                                                                                                       <  2:8143/140‑1 (MQ=255)
     tGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGc                                                                                                                     >  1:491588/1‑140 (MQ=255)
      gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    >  2:219519/1‑140 (MQ=255)
      gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    >  1:545038/1‑140 (MQ=255)
      gCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCg                                                                                                                    <  1:76166/140‑1 (MQ=255)
            cggtcggGAATGTCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCgaga                                                                                                              <  1:457649/140‑1 (MQ=255)
              gtcggGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGt                                                                                                            >  1:159526/1‑140 (MQ=255)
                                                 aaCAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCcgc                                                                         >  2:555704/1‑140 (MQ=255)
                                                      ccTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGaa                                                                    >  1:341197/1‑140 (MQ=255)
                                                          cGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATcg                                                                >  1:74088/1‑140 (MQ=255)
                                                                 ttGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCCTTACGGCAGATAATTTTGCCGCGAGAAATcgccgccgc                                                         <  1:91896/140‑1 (MQ=255)
                                                                    ccATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGccac                                                      <  1:28177/140‑1 (MQ=255)
                                                                               cAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTAc                                           <  1:392831/140‑1 (MQ=255)
                                                                                 ggCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCg                                         >  1:106203/1‑140 (MQ=255)
                                                                                  gCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCgg                                        >  2:282478/1‑140 (MQ=255)
                                                                                  gCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCgg                                        >  2:338467/1‑140 (MQ=255)
                                                                                   cTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGt                                       <  2:545038/140‑1 (MQ=255)
                                                                                     aaTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGttt                                     <  1:589539/140‑1 (MQ=255)
                                                                                      aTCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTc                                    >  2:93991/1‑140 (MQ=255)
                                                                                          aGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATa                                <  2:472175/140‑1 (MQ=255)
                                                                                                cGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATcc                          >  2:324093/1‑140 (MQ=255)
                                                                                                        tCGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAAc                  >  2:3081/1‑140 (MQ=255)
                                                                                                         cGATGTCCGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAAcg                 <  2:341197/140‑1 (MQ=255)
                                                                                                               ccGCTCCAGCGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAg           >  2:17984/1‑140 (MQ=255)
                                                                                                                        cGTCACGCAGTTTCTCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACAccgccg  >  1:60291/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                             
GGGCCTGCATATCGGTCGGGAATGCCGGATGCGGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCG  >  NC_000913/3335570‑3335829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A