Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,604,429	C→A	R13S (CGC→AGC)	rsmD →	16S rRNA m(2)G966 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,604,429	0	C	A	100.0%	205.8 / NA	58	R13S (CGC→AGC)	rsmD	16S rRNA m(2)G966 methyltransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (30/28); total (30/28)

TGTACATAGCAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGT  >  NC_000913/3604292‑3604560

tGTACATAGCAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGc                                                                                                                                   >  1:280527/1‑140 (MQ=255)

      tAGCAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCattatt                                                                                                                             >  1:787284/1‑140 (MQ=255)

       agcagcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTg                                                                                                                            >  1:466794/1‑140 (MQ=255)

        gcagcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTgg                                                                                                                           >  1:609506/1‑140 (MQ=255)

        gcagcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTgg                                                                                                                           >  2:354632/1‑140 (MQ=255)

          agcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGTGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGc                                                                                                                          >  1:735116/1‑139 (MQ=255)

          agcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGTGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGc                                                                                                                          <  2:735116/139‑1 (MQ=255)

          agcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCg                                                                                                                         >  2:790794/1‑140 (MQ=255)

          agcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCg                                                                                                                         >  1:513040/1‑140 (MQ=255)

          agcTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGACCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCg                                                                                                                         >  1:107539/1‑140 (MQ=255)

             tAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGc                                                                                                                      <  1:6053/140‑1 (MQ=255)

                                    tCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCg                                                                                               >  2:215204/1‑140 (MQ=255)

                                    tCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCg                                                                                               >  1:527180/1‑140 (MQ=255)

                                       cttaacttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGtt                                                                                            >  1:745500/1‑140 (MQ=255)

                                       cttaacttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGtt                                                                                            >  2:758655/1‑140 (MQ=255)

                                         taacttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTcc                                                                                          >  1:744072/1‑140 (MQ=255)

                                          aacttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCt                                                                                         >  1:10009/1‑140 (MQ=255)

                                          aacttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCt                                                                                         >  1:169093/1‑140 (MQ=255)

                                            cttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGa                                                                                       <  2:787284/140‑1 (MQ=255)

                                            cttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGa                                                                                       <  1:758655/140‑1 (MQ=255)

                                            cttaaTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGa                                                                                       <  2:107539/140‑1 (MQ=255)

                                                aTTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGc                                                                                   >  1:95784/1‑140 (MQ=255)

                                                 ttCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGa                                                                                       <  2:320444/135‑1 (MQ=255)

                                                 ttCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGa                                                                                       >  1:320444/1‑135 (MQ=255)

                                                  tCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGccc                                                                                 >  1:20711/1‑140 (MQ=255)

                                                  tCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACCCCCGGTTCCTGATAGccc                                                                                 >  2:204606/1‑140 (MQ=255)

                                                     caTCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCCGTGGCGCGGCCGTTAACTCCCCGTTCCCGCTAACCCAgg                                                                              >  2:482443/1‑140 (MQ=255)

                                                      aTCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTTATAGCCCAGGt                                                                             >  1:171730/1‑140 (MQ=255)

                                                         aCCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTg                                                                          <  1:354632/140‑1 (MQ=255)

                                                         aCCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTg                                                                          <  2:257609/140‑1 (MQ=255)

                                                         aCCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTg                                                                          <  2:555114/140‑1 (MQ=255)

                                                         aCCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTg                                                                          <  2:825309/140‑1 (MQ=255)

                                                         aCCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTg                                                                          <  2:613328/140‑1 (MQ=255)

                                                           cGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTgcg                                                                        >  2:762655/1‑140 (MQ=255)

                                                             ccTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGcc                                                                      <  2:745500/140‑1 (MQ=255)

                                                                 cAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCccac                                                                  <  2:68633/140‑1 (MQ=255)

                                                                  aaGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCccacc                                                                 <  2:10009/140‑1 (MQ=255)

                                                                     tATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCaccacc                                                              >  2:84258/1‑140 (MQ=255)

                                                                        gtgtTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCaccgac                                                           <  2:280527/140‑1 (MQ=255)

                                                                             aTCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGGCGTAAACTCCCGGGTTCTGATAGGCCAGGGCTGGGCCCCACCACCGACcgcct                                                      >  2:384097/1‑138 (MQ=255)

                                                                              tCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTGAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTa                                                     >  2:377066/1‑140 (MQ=255)

                                                                              tCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTa                                                     >  2:28548/1‑140 (MQ=255)

                                                                                               tttATCATGAAAAAACCGAATCATTCCGGCAGCGGTCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTaa                                    <  2:609506/140‑1 (MQ=255)

                                                                                               tttATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTaa                                    <  2:466794/140‑1 (MQ=255)

                                                                                               tttATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTaa                                    <  2:20711/140‑1 (MQ=255)

                                                                                                    cATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAActggc                               <  2:341300/140‑1 (MQ=255)

                                                                                                    cATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAACCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAActggc                               >  2:551506/1‑140 (MQ=255)

                                                                                                     aTGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAActggct                              <  1:215204/140‑1 (MQ=255)

                                                                                                         aaaaaCCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTggctggct                          <  2:887542/140‑1 (MQ=255)

                                                                                                          aaaaCCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTc                         <  1:762655/140‑1 (MQ=255)

                                                                                                             aCCGAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCgg                      >  1:330619/1‑140 (MQ=255)

                                                                                                                gAATCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCGGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCa                   <  2:392578/140‑1 (MQ=255)

                                                                                                                 aaTCATTCCGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCAt                  <  1:165368/140‑1 (MQ=255)

                                                                                                                        ccGGCAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGAc           <  1:790794/140‑1 (MQ=255)

                                                                                                                            cAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGccc       <  2:169093/140‑1 (MQ=255)

                                                                                                                            cAGCGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGccc       <  1:84258/140‑1 (MQ=255)

                                                                                                                               cGGCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAAt    >  1:388086/1‑140 (MQ=255)

                                                                                                                                 gCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGt  >  1:884488/1‑140 (MQ=255)

                                                                                                                                 gCCAAATCAGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGt  <  1:651248/140‑1 (MQ=255)

TGTACATAGCAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCTGGCGGATTGAGCAATTTATCATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCCTGATAGCCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACGCCCAATGT  >  NC_000913/3604292‑3604560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A