Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F73 I1 R1 27 19.6 661800 98.9% 654520 139.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,120,882 G→T A133D (GCT→GAT)  glcA ← glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,120,8820GT100.0% 70.4 / NA 21A133D (GCT→GAT) glcAglycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/12);  total (9/12)

CGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTA  >  NC_000913/3120764‑3121007
                                                                                                                      |                                                                                                                             
cGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCAc                                                                                                          <  1:253711/140‑1 (MQ=255)
           ccACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGaaa                                                                                               <  2:100555/140‑1 (MQ=255)
           ccACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGaaa                                                                                               <  1:30322/140‑1 (MQ=255)
            cACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAc                                                                                              <  1:102267/140‑1 (MQ=255)
             aCCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAAcc                                                                                             <  1:230766/140‑1 (MQ=255)
                        aTTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                                                                                  >  1:68669/1‑140 (MQ=255)
                                 cAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAAc                                                                         >  1:11755/1‑140 (MQ=255)
                                          ccccGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                                                                                  <  1:243665/122‑1 (MQ=255)
                                          ccccGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAaca                                                                                  >  2:243665/1‑122 (MQ=255)
                                          ccccGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcgt                                                                >  2:182421/1‑140 (MQ=255)
                                            ccgccgCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGAtcgtcg                                                              <  2:68669/140‑1 (MQ=255)
                                              gccgcGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGt                                                            >  1:225343/1‑140 (MQ=255)
                                                              tGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATAGCCACCGGCGCACCAAAGCCATCCGCCCCTTCCAGCAACGCACCAAAGGAGAAACCAAACAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAg                                            >  2:220588/1‑140 (MQ=255)
                                                                aaGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCt                                          <  1:134574/140‑1 (MQ=255)
                                                                     cAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGa                                     <  1:297253/140‑1 (MQ=255)
                                                                     cAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGa                                     <  2:262874/140‑1 (MQ=255)
                                                                             ccaGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCg                             >  1:69651/1‑140 (MQ=255)
                                                                                   gcgcACCGGTAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGc                       >  2:117539/1‑140 (MQ=255)
                                                                                        ccGGTAATCGCCACCGGCGAACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCt                  >  2:17857/1‑140 (MQ=255)
                                                                                            tAATCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCa              <  1:129675/140‑1 (MQ=255)
                                                                                              aTCGCCACCGGCGCACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAAc            >  1:213044/1‑140 (MQ=255)
                                                                                                        gcgcACCAAAGCCATCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTta  <  2:30984/140‑1 (MQ=255)
                                                                                                                      |                                                                                                                             
CGCACCAAACGCCACCGGCGCAGTATTGGCAATCAGACACAGCCCCGCCGCGTATAACGGTTTGAAGCCCAGGCCCACCAGCAGCGCACCGGTAATCGCCACCGGCGCACCAAAGCCAGCCGCTCCTTCCAGCAACGCACCAAAGGAGAAACCAATCAGTAACACCTGCAAACGCTGATCGTCGGTGATGGAGATAACCGAGCTGCGGATAATATCGAACTGCCCGCTGGCAACGGTTAATTTA  >  NC_000913/3120764‑3121007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A