Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F73 I1 R1	27	19.6	661800	98.9%	654520	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,851	G→T	V141F (GTT→TTT)	udp →	uridine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,851	0	G	T	100.0%	87.1 / NA	26	V141F (GTT→TTT)	udp	uridine phosphorylase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (13/13); total (13/13)

CGGGCGCTATTCAGCCGCATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAA  >  NC_000913/4016714‑4016985

cGGGCGCTATTCAGCCGCATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGttt                                                                                                                                      >  1:227024/1‑140 (MQ=255)

  ggCGCTATTCAGCCGCATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGa                                                                                                                                    >  2:100755/1‑140 (MQ=255)

   gcgcTATTCAGCCGCATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGaa                                                                                                                                   >  2:218627/1‑140 (MQ=255)

                   tatTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTg                                                                                                                   >  2:211996/1‑140 (MQ=255)

                            ggggGATGCCCTGGTTACCACGGCGTCTGTCCGCCTGGATGGCGCGCGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACaa                                                                                                          >  2:168050/1‑140 (MQ=255)

                             ggTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAAc                                                                                                         >  2:18402/1‑140 (MQ=255)

                             ggTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAAc                                                                                                         >  1:97103/1‑140 (MQ=255)

                             ggTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAAc                                                                                                         >  2:290297/1‑140 (MQ=255)

                                          ttACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTg                                                                                            <  2:67433/140‑1 (MQ=255)

                                              cACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAg                                                                                        >  1:87920/1‑140 (MQ=255)

                                              cACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAg                                                                                        >  1:170481/1‑140 (MQ=255)

                                                cGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGct                                                                                      >  1:143782/1‑140 (MQ=255)

                                                 ggCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGctt                                                                                     <  1:117019/140‑1 (MQ=255)

                                                       tGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTg                                                                               <  1:233916/140‑1 (MQ=255)

                                                        gTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGa                                                                              <  1:271572/140‑1 (MQ=255)

                                                         tCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGAt                                                                             <  2:170481/140‑1 (MQ=255)

                                                                      cgcgAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAg                                                                <  1:218627/140‑1 (MQ=255)

                                                                            ccTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAgg                                                          <  1:211996/140‑1 (MQ=255)

                                                                                cACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACg                                                      <  1:56781/140‑1 (MQ=255)

                                                                                   ttCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTa                                                   <  2:97103/140‑1 (MQ=255)

                                                                                    tCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTAc                                                  <  2:87920/140‑1 (MQ=255)

                                                                                    tCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTAc                                                  <  1:100755/140‑1 (MQ=255)

                                                                                     cGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACg                                                 <  2:143782/140‑1 (MQ=255)

                                                                                       cACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGAt                                               >  2:311248/1‑140 (MQ=255)

                                                                                             tGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGAtacttac                                         >  1:103630/1‑140 (MQ=255)

                                                                                                         cTGTCGCTGATTTCGAATGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTa                             >  2:204425/1‑140 (MQ=255)

                                                                                                                          tGTACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAgg            <  2:227024/140‑1 (MQ=255)

                                                                                                                            tACGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTTCTCTGGTCGCGTAGTTCGTCACTTTAAAGGtt          <  2:14763/140‑1 (MQ=255)

                                                                                                                              cGACTGCGCTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCt        >  1:56145/1‑140 (MQ=255)

                                                                                                                                    cgcTGTTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGaa  >  1:285397/1‑140 (MQ=255)

CGGGCGCTATTCAGCCGCATATTAATGTGGGTGATGTCCTGGTTACCACGGCGTCTGTCCGTCTGGATGGCGCGAGCCTGCACTTCGCACCGCTGGAATTCCCGGCTGTCGCTGATTTCGAATGTACGACTGCGCTGGTTGAAGCTGCGAAATCCATTGGCGCGACAACTCACGTTGGCGTGACAGCTTCTTCTGATACCTTCTACCCAGGTCAGGAACGTTACGATACTTACTCTGGTCGCGTAGTTCGTCACTTTAAAGGTTCTATGGAA  >  NC_000913/4016714‑4016985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A