Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F73 I1 R1
|
26 |
53.7 |
1833324 |
99.1% |
1816824 |
139.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,213,082 |
C→A |
noncoding (43/120 nt) |
rrfE → |
5S ribosomal RNA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,213,082 | 0 | C | A | 100.0%
| 77.4
/ NA
| 24 | noncoding (43/120 nt) | rrfE | 5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/10); total (14/10) |
AAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCA > NC_000913/4213030‑4213217
|
aaaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaa > 2:49866/1‑140 (MQ=18)
aTTTGCCTGGCGGCCGTAGCGCGGCGGTCCCACCTGAGCCCATGCCGCACTCAGAAGTGAAACGCCGTAGCGCCGATGGTTGCGCGGGGTCTCCCCATGCGAGAGCAGGGAACTGCCAGCCATCAACTTAGAAAAAcccc > 2:62951/1‑140 (MQ=11)
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAACcccc > 2:654573/1‑140 (MQ=255)
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcccc > 1:296348/1‑140 (MQ=255)
aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcccc > 1:442323/1‑140 (MQ=255)
tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg < 2:442323/140‑1 (MQ=255)
tttGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCg < 1:203348/140‑1 (MQ=255)
gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa > 2:485765/1‑140 (MQ=255)
gcggcAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATaa > 2:476973/1‑140 (MQ=255)
aGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg < 2:206991/140‑1 (MQ=255)
aGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg > 2:303582/1‑140 (MQ=255)
tggtCCCCCGTGATCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGGTTTTTg < 2:841974/140‑1 (MQ=17)
tggtCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg < 2:36117/140‑1 (MQ=255)
tggtCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg < 2:860810/140‑1 (MQ=255)
tggtCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTg < 1:906292/140‑1 (MQ=255)
gtCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCa < 2:880240/140‑1 (MQ=255)
tCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCat < 2:688110/140‑1 (MQ=255)
cTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAat > 1:905743/1‑140 (MQ=255)
aCCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattat > 2:567050/1‑140 (MQ=255)
aCCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattat > 1:671839/1‑140 (MQ=255)
aCCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattat > 1:488578/1‑140 (MQ=255)
aCCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattat > 2:65617/1‑140 (MQ=255)
aCCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattat > 1:410444/1‑140 (MQ=255)
cATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGc < 1:769607/140‑1 (MQ=255)
cATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGc < 1:303582/140‑1 (MQ=255)
aTGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa > 1:674259/1‑140 (MQ=255)
|
AAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCA > NC_000913/4213030‑4213217
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
