breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 199,188 C→A R421S (CGC→AGC)  bamA → outer membrane protein assembly factor BamA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 339,025 C→A A234E (GCG→GAG)  yahG → DUF1116 domain‑containing protein YahG
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,498,390 C→A P512Q (CCG→CAG)  opgD → glucan biosynthesis protein D
RA 1,528,418 C→A pseudogene (529/2049 nt) rhsE → RhsE protein in rhs element
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,881,732 C→A A26A (GCG→GCT dmlR ← DNA‑binding transcriptional regulator DmlR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,374,861 C→A G48V (GGG→GTG)  menE ← o‑succinylbenzoate‑‑CoA ligase
RA 2,945,626 G→T Q73K (CAA→AAA)  tcdA ← tRNA threonylcarbamoyladenosine dehydratase
RA 2,952,852 G→T P1051Q (CCG→CAG)  recB ← exodeoxyribonuclease V subunit RecB
RA 3,068,651 G→T A53D (GCC→GAC)  argO ← L‑arginine exporter
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,384,420 C→A intergenic (‑152/‑283) mdh ← / → argR malate dehydrogenase/DNA‑binding transcriptional dual regulator ArgR
RA 3,464,116 C→A S65S (TCC→TCA gspL → Type II secretion system protein GspL
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,824,905 G→T A131S (GCC→TCC)  trmH → tRNA (Gm18) 2'‑O‑methyltransferase
RA 4,213,082 C→A noncoding (43/120 nt) rrfE → 5S ribosomal RNA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,443,534 C→A L385M (CTG→ATG)  tamA → translocation and assembly module subunit TamA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102995 3104069 1075 12 [10] [10] 12 mutY mutY
* * ÷ NC_000913 3423791–3424234 3424526–3424243 10–736 12 [8] [11] 12 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374922 =NA (NA)24 (0.470) 9/260 3.0 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)9 (0.240) 8/190 2.3 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375023 =NA (NA)13 (0.350) 6/192 2.9 NA intergenic (+142/‑436) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 411076 =NA (NA)16 (0.300) 12/274 2.5 NA intergenic (+24/+221) mak/araJ manno(fructo)kinase/putative transport protein AraJ
?NC_000913 = 4296058 NA (NA)intergenic (+264/+378) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
* ? NC_000913 1207790 =16 (0.300)66 (1.380) 43/244 0.1 77.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 24 (0.500)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780516 (0.300)37 (0.770) 26/244 0.6 66.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 24 (0.500)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)76 (1.450) 48/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =75 (1.390)79 (1.470) 50/274 0.1 67.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)67 (1.240) 43/276 0.2 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4373295 =NA (NA)19 (0.380) 9/256 2.9 NA noncoding (2/34 nt) other REP328b
?NC_000913 4374499 = NA (NA)noncoding (1/34 nt) other REP329a