Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,149	C→A	L596M (CTG→ATG)	yejA →	putative oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,149	0	C	A	95.6%	144.4 / ‑1.8	45	L596M (CTG→ATG)	yejA	putative oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (25/18); minor base G (1/1); total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

TGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGG  >  NC_000913/2274021‑2274281

tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc                                                                                                                            >  1:118203/1‑140 (MQ=255)

tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc                                                                                                                            >  1:170295/1‑140 (MQ=255)

tgctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCCGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGcc                                                                                                                            >  1:668982/1‑140 (MQ=255)

  ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg                                                                                                                          >  2:459950/1‑140 (MQ=255)

  ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg                                                                                                                          <  2:4254/140‑1 (MQ=255)

  ctgcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAg                                                                                                                          >  1:107266/1‑140 (MQ=255)

    gcCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCa                                                                                                                        >  1:490361/1‑140 (MQ=255)

     ccAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCaa                                                                                                                       >  2:77683/1‑140 (MQ=255)

         tgtgGCACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCCGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAg                                                                                                                   <  2:668982/140‑1 (MQ=255)

                   ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGa                                                                                                         >  2:77064/1‑140 (MQ=255)

                    gCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGAt                                                                                                        <  1:77064/140‑1 (MQ=255)

                     cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg                                                                                                       >  2:113624/1‑140 (MQ=255)

                     cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg                                                                                                       >  1:198558/1‑140 (MQ=255)

                     cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATg                                                                                                       >  1:401507/1‑140 (MQ=255)

                     cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGGGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCACACAGGGAGAGTAGATg                                                                                                       >  2:359184/1‑140 (MQ=255)

                               tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAGTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                                                                             >  2:517262/1‑140 (MQ=255)

                               tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                                                                             >  2:395580/1‑140 (MQ=255)

                               tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTAcc                                                                                             >  2:688586/1‑140 (MQ=255)

                                         tggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCg                                                                                   <  1:463935/140‑1 (MQ=255)

                                          gggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGt                                                                                  <  2:215308/140‑1 (MQ=255)

                                                  ttCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCt                                                                          <  1:675759/140‑1 (MQ=255)

                                                          gccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATcc                                                                  >  1:238456/1‑140 (MQ=255)

                                                           ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                 <  1:77683/140‑1 (MQ=255)

                                                           ccggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                 <  2:238456/140‑1 (MQ=255)

                                                                   gcgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                         <  2:620788/140‑1 (MQ=255)

                                                                    cgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATg                                                        <  2:190771/140‑1 (MQ=255)

                                                                     gcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATgg                                                       <  1:60073/140‑1 (MQ=255)

                                                                      ccccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                      <  2:198558/140‑1 (MQ=255)

                                                                      ccccATCTATAGCCTCGGTATCGATACCTGGTGGTACGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATggg                                                      <  1:170451/140‑1 (MQ=255)

                                                                       cccATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGc                                                     <  2:571062/140‑1 (MQ=255)

                                                                             tataGCCTCGGTATCGATTCCTGGTGGTAGGACGTCAATAGAGCGGCCAAAATGCCGGCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCATCATTATGGGCGATTAt                                               <  2:158264/140‑1 (MQ=255)

                                                                                    tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAGTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                        <  1:517262/140‑1 (MQ=255)

                                                                                    tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                        <  1:113624/140‑1 (MQ=255)

                                                                                    tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                        >  1:462256/1‑140 (MQ=255)

                                                                                     cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑a                                      >  1:35920/1‑140 (MQ=255)

                                                                                     cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑a                                      >  1:65543/1‑140 (MQ=255)

                                                                                      ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑aa                                     >  1:192281/1‑140 (MQ=255)

                                                                                             aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAAATCCCGTCCCCCAGAAAACAGGGGGGGAGGATGGCGCCTTACTTATTTGGCCGCCTGTTCCTGGGGATCCCACCATTTTGGCCGTTTAT‑ACCATCAAACTTTTTc                              >  2:629638/1‑140 (MQ=255)

                                                                                                ccTGGTGGTATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATc                           >  2:550354/1‑140 (MQ=255)

                                                                                                    gtggtATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGc                       >  1:607043/1‑140 (MQ=255)

                                                                                                       gtATGACGTCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCaaa                    >  2:293515/1‑140 (MQ=255)

                                                                                                               tCAATAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCt            >  1:499787/1‑140 (MQ=255)

                                                                                                                 aaTAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTgg          >  2:626655/1‑140 (MQ=255)

                                                                                                                   tAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcg        <  2:462256/140‑1 (MQ=255)

                                                                                                                   tAAAGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcg        >  2:553000/1‑140 (MQ=255)

                                                                                                                     aaGCGGCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggt      >  1:663750/1‑140 (MQ=255)

                                                                                                                         ggCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg  <  1:407471/140‑1 (MQ=255)

                                                                                                                         ggCCAAAATGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg  <  2:663750/140‑1 (MQ=255)

TGCTGCCAATGTGGTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATC‑AACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGG  >  NC_000913/2274021‑2274281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A