Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,483,187	C→A	T51T (ACG→ACT)	emrK ←	tripartite efflux pump membrane fusion protein EmrK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,483,187	0	C	A	100.0%	106.9 / NA	32	T51T (ACG→ACT)	emrK	tripartite efflux pump membrane fusion protein EmrK
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/15); total (17/15)

GCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAT  >  NC_000913/2483050‑2483320

gCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGt                                                                                                                                     >  2:407506/1‑140 (MQ=255)

    tATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACa                                                                                                                                 >  2:8574/1‑140 (MQ=255)

         aGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGc                                                                                                                            <  2:386876/140‑1 (MQ=255)

              gATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCAt                                                                                                                       <  2:456839/140‑1 (MQ=255)

                   tGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTa                                                                                                                  >  1:375974/1‑140 (MQ=255)

                       aTCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTaa                                                                                                              <  2:188571/140‑1 (MQ=255)

                            ttttATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATg                                                                                                         <  1:186620/140‑1 (MQ=255)

                                 tCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGtcttc                                                                                                    >  1:422843/1‑140 (MQ=255)

                                   cAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGtcttctt                                                                                                  <  2:29886/140‑2 (MQ=255)

                                    aGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTaa                                                                                                 >  1:96723/1‑140 (MQ=255)

                                           cTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCAtt                                                                                          <  1:116268/140‑1 (MQ=255)

                                           cTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCAtt                                                                                          <  1:407506/140‑1 (MQ=255)

                                                aTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGGCCa                                                                                     <  1:71940/140‑1 (MQ=255)

                                                aTGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCa                                                                                     >  1:158771/1‑140 (MQ=255)

                                                 tGTCTCGTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTTCCAGTGACATAGGCGTCATCTGTTCTAATCATGTCTTCTAATTCCATTGACCaa                                                                                    <  1:357464/140‑1 (MQ=255)

                                                   tCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAata                                                                                  <  2:228178/140‑1 (MQ=255)

                                                                        gTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGc                                                             <  2:577428/140‑1 (MQ=255)

                                                                         tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:181021/140‑1 (MQ=255)

                                                                         tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:251286/140‑1 (MQ=255)

                                                                         tATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCa                                                            <  2:34382/140‑1 (MQ=255)

                                                                               tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaat                                                      >  2:25843/1‑140 (MQ=255)

                                                                                       aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAc                                              >  1:172510/1‑140 (MQ=255)

                                                                                       aCGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAc                                              >  1:614123/1‑140 (MQ=255)

                                                                                              tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                       >  2:357601/1‑140 (MQ=255)

                                                                                              tGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCATCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAAt                                       >  1:243063/1‑140 (MQ=255)

                                                                                                  acTACCTGAGACTTGTGCAAAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg                                   <  2:552127/140‑1 (MQ=255)

                                                                                                      ccTGAGACTTGCGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAACTAAAACTACCGCCAATAACGaaaa                               >  2:522422/1‑140 (MQ=255)

                                                                                                          agaCTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAt                           >  1:92921/1‑140 (MQ=255)

                                                                                                            aCTTGTGCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAttt                         >  2:423650/1‑140 (MQ=255)

                                                                                                                gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  1:539131/1‑140 (MQ=255)

                                                                                                                gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  1:299685/1‑140 (MQ=255)

                                                                                                                gtgCAGAAATTGGATCTGCATTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt                     >  2:504388/1‑140 (MQ=255)

                                                                                                                 tgCAAAAATTGGATCTGCATCCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGAATAGGCCCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc                    <  2:55441/140‑1 (MQ=255)

                                                                                                                                   aTTCCCAGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGCAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt  <  2:65048/140‑1 (MQ=255)

GCTTTATTGAGTGCGATAGTGGCATCAGTTTTATCCAGTGAAACTAAAATGTCACCTTGTCGAACGTAGTTCGTATCTTTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAT  >  NC_000913/2483050‑2483320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A