Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F73 I1 R1
|
30 |
41.4 |
1413620 |
99.2% |
1402311 |
139.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,023,896 |
G→T |
G861C (GGC→TGC) |
xdhD → |
fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,023,896 | 0 | G | T | 100.0%
| 82.9
/ NA
| 25 | G861C (GGC→TGC) | xdhD | fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/15); total (10/15) |
ACTTCGCTGAAGTTGCCGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGA > NC_000913/3023759‑3024026
|
aCTTCGCTGAAGTTGCCGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGc < 1:82397/140‑1 (MQ=255)
gCCGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGa < 2:132576/140‑1 (MQ=255)
ccGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGaa < 2:239831/140‑1 (MQ=255)
ccGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGaa < 1:530379/140‑1 (MQ=255)
gTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAaga < 1:272336/140‑1 (MQ=255)
gTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTAt < 1:21993/140‑1 (MQ=255)
ggTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGc < 1:475195/140‑1 (MQ=255)
ccTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCAcc > 2:642111/1‑140 (MQ=255)
gATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGtt > 1:265607/1‑140 (MQ=255)
aTTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAAcgc < 1:92160/140‑1 (MQ=255)
ttGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACg < 1:426968/140‑1 (MQ=255)
ttGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACg < 1:642111/140‑1 (MQ=255)
cGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGgcg > 1:235555/1‑140 (MQ=255)
gTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCa < 2:265607/140‑1 (MQ=255)
cacCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCg > 2:83303/1‑140 (MQ=255)
cGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGc < 2:249320/122‑1 (MQ=255)
cGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGc > 1:249320/1‑122 (MQ=255)
tCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTg > 2:182692/1‑140 (MQ=255)
tCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCAAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTg > 2:470912/1‑140 (MQ=255)
aGCGTTGGGACAAATCTACGGTGCCTCCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgc < 1:582243/140‑1 (MQ=255)
gCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCcgcg > 1:693549/1‑140 (MQ=255)
aTCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc < 2:322732/140‑1 (MQ=255)
aTCTACGGTGCCACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCgcgc < 2:693549/140‑1 (MQ=255)
ccACCCTGCGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTg > 2:354684/1‑140 (MQ=255)
cGTGCTATCTGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcga > 1:132425/1‑140 (MQ=255)
|
ACTTCGCTGAAGTTGCCGTCAACACGCGTACGGGTGAAATCCGCCTGGATAAATTCTACGCCTTGCTGGACTGCGGTACACCGGTCAATCCAGAGTTAGCGTTGGGACAAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGA > NC_000913/3023759‑3024026
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
