Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F73 I1 R1 30 41.4 1413620 99.2% 1402311 139.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,750,633 C→A A183D (GCC→GAC)  sgbE → L‑ribulose‑5‑phosphate 4‑epimerase SgbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,750,6330CA100.0% 90.0 / NA 28A183D (GCC→GAC) sgbEL‑ribulose‑5‑phosphate 4‑epimerase SgbE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/16);  total (12/16)

ATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAT  >  NC_000913/3750505‑3750763
                                                                                                                                |                                                                                                                                  
atatgaatatCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACg                                                                                                                         <  1:172788/140‑1 (MQ=255)
         tCAGACCGGCGAAGTGATCATTGAAACCTTCGGAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTAc                                                                                                                >  1:702738/1‑140 (MQ=255)
             aCCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCga                                                                                                            >  1:464115/1‑140 (MQ=255)
                 gCGAAGTGATCATTGAAACCTTCGGAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        <  2:702738/140‑1 (MQ=255)
                 gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        <  2:91964/140‑1 (MQ=255)
                 gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGGAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaa                                                                                                        >  2:444563/1‑140 (MQ=255)
                 gCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACAGCCCGTTCGCCTGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCggagaa                                                                                                        >  2:509360/1‑140 (MQ=255)
                  cGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCgaagaaa                                                                                                       >  2:56272/1‑139 (MQ=255)
                           cATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCtata                                                                                              <  2:117156/140‑1 (MQ=255)
                           cATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCtata                                                                                              <  1:182420/140‑1 (MQ=255)
                                     ttCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGATGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTAtt                                                                                    >  1:594390/1‑140 (MQ=255)
                                     ttCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGAAGGGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTAtt                                                                                    >  2:524114/1‑140 (MQ=255)
                                              cGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCa                                                                           >  1:676157/1‑140 (MQ=255)
                                                tGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGc                                                                         <  1:245901/140‑1 (MQ=255)
                                                   cAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTg                                                                      <  1:509360/140‑1 (MQ=255)
                                                          ccGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCa                                                               <  2:298493/140‑1 (MQ=255)
                                                           cGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAg                                                              <  1:425402/140‑1 (MQ=255)
                                                                       cggcggTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATg                                                  >  1:59244/1‑140 (MQ=255)
                                                                                tGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGaa                                         <  1:326130/140‑1 (MQ=255)
                                                                                    gCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAActgc                                     <  1:497998/140‑1 (MQ=255)
                                                                                     cATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACTCCATAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCGGCAGCTCCCTGCGATGCAAAACGAActgct                                    <  1:703114/140‑1 (MQ=255)
                                                                                                cccGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACt                         >  2:346784/1‑140 (MQ=255)
                                                                                                  ctgtCGCATGGGGAAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACGCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCCGCTTGCGTCGCAGCGCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  2:489899/137‑1 (MQ=255)
                                                                                                  cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  2:247/140‑1 (MQ=255)
                                                                                                  cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       >  2:535457/1‑140 (MQ=255)
                                                                                                  cGTTCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTAc                       <  1:603748/140‑1 (MQ=255)
                                                                                                    ttCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCt                     >  2:222613/1‑140 (MQ=255)
                                                                                                    ttCGCATGGGGTAAAAACGCCGCCGATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCCGCGATGCAAAACGAACTGCTGGATAAGCACTACCt                     >  1:246252/1‑140 (MQ=255)
                                                                                                        cATGGGGTAAAAACGCCGCCGATGACGTACATAACGCCGTAGTACTCGAGGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGt                 <  1:263682/140‑1 (MQ=255)
                                                                                                                       ccgccgATGACGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAt  <  1:688212/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
ATATGAATATCAGACCGGCGAAGTGATCATTGAAACCTTCGAAGAACGTGGCAGGAGTCCGGCACAAATCCCGGCGGTGCTGGTGCATTCTCACGGCCCGTTCGCATGGGGTAAAAACGCCGCCGATGCCGTGCATAACGCCGTAGTACTCGAAGAATGCGCCTATATGGGTCTATTCTCGCGCCAGCTTGCGCCGCAGCTCCCTGCGATGCAAAACGAACTGCTGGATAAGCACTACCTGCGTAAGCATGGGGCCAAT  >  NC_000913/3750505‑3750763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A