Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	31	34.8	1203930	99.1%	1193094	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,172,161	C→A	M17I (ATG→ATT)	ygiZ ←	conserved inner membrane protein YgiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,172,161	0	C	A	100.0%	175.7 / NA	51	M17I (ATG→ATT)	ygiZ	conserved inner membrane protein YgiZ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (32/19); total (32/19)

GCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCAAAGCCA  >  NC_000913/3172030‑3172292

gctgcAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATtatata                                                                                                                             >  2:164520/1‑140 (MQ=255)

gctgcAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATtatata                                                                                                                             >  1:271211/1‑140 (MQ=255)

     aaTCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCATAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCa                                                                                                                        <  2:589352/140‑1 (MQ=255)

                 cAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAata                                                                                                            >  1:148383/1‑140 (MQ=255)

                        cGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGtttt                                                                                                     >  1:483129/1‑140 (MQ=255)

                          aTCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTaa                                                                                                   <  1:590352/140‑1 (MQ=255)

                              tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAAtttt                                                                                               >  1:202629/1‑140 (MQ=255)

                              tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAAtttt                                                                                               >  2:17094/1‑140 (MQ=255)

                              tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAAtttt                                                                                               <  2:84079/140‑1 (MQ=255)

                               cGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTc                                                                                              >  2:315318/1‑140 (MQ=255)

                                gCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCt                                                                                             >  2:231614/1‑140 (MQ=255)

                                gCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCt                                                                                             >  1:566454/1‑140 (MQ=255)

                                   tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCt                                                                                          <  1:581237/140‑1 (MQ=255)

                                    gAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                                                         <  1:121386/140‑1 (MQ=255)

                                    gAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                                                         <  1:213996/140‑1 (MQ=255)

                                    gAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                                                         <  1:235218/140‑1 (MQ=255)

                                     aaaCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCttt                                                                                        >  2:180597/1‑140 (MQ=255)

                                         ggAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACa                                                                                    <  1:2311/140‑1 (MQ=255)

                                         ggAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACa                                                                                    <  1:493653/140‑1 (MQ=255)

                                           aaGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACata                                                                                  <  1:575745/140‑1 (MQ=255)

                                                    tGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTAc                                                                         >  2:81718/1‑140 (MQ=255)

                                                    tGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTAc                                                                         >  2:233692/1‑140 (MQ=255)

                                                     gCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACg                                                                        >  2:64063/1‑140 (MQ=255)

                                                     gCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACg                                                                        >  1:212165/1‑140 (MQ=255)

                                                      cAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                                                     >  1:472583/1‑126 (MQ=255)

                                                      cAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                                                     <  2:472583/126‑1 (MQ=255)

                                                      cAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAC‑TATATCAGTACGCt                                                                      >  2:145184/1‑140 (MQ=255)

                                                         atatTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGGAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCa                                                                    >  1:440887/1‑140 (MQ=255)

                                                         atatTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCa                                                                    >  2:199911/1‑140 (MQ=255)

                                                         atatTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCa                                                                    >  1:215381/1‑140 (MQ=255)

                                                         atatTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCa                                                                    <  1:597932/140‑1 (MQ=255)

                                                                     gACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGAACGCTCATAAAAATCAAtt                                                        >  2:157547/1‑140 (MQ=255)

                                                                       cTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTta                                                      >  1:291763/1‑140 (MQ=255)

                                                                            gCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAAtt                                                 <  1:486699/140‑1 (MQ=255)

                                                                                      aTACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGa                                       <  1:315318/140‑1 (MQ=255)

                                                                                          ttATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACg                                   >  2:210478/1‑140 (MQ=255)

                                                                                              tGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGa                               >  1:88025/1‑140 (MQ=255)

                                                                                              tGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGa                               >  1:285623/1‑140 (MQ=255)

                                                                                              tGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGa                               >  2:398405/1‑140 (MQ=255)

                                                                                               gAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGAt                              <  2:483129/140‑1 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  2:84541/1‑140 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  2:508616/1‑140 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  1:513293/1‑140 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  1:461596/1‑140 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  1:448423/1‑140 (MQ=255)

                                                                                                  aGCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACt                           >  1:228032/1‑140 (MQ=255)

                                                                                                   gCAAAAAAAGAATCTATTAACCAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACtt                          <  2:122699/140‑1 (MQ=255)

                                                                                                                       ccAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCaaa      <  2:476210/140‑1 (MQ=255)

                                                                                                                       ccAATAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCaaa      <  1:398405/140‑1 (MQ=255)

                                                                                                                         aaTAAGTAAGAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAC‑TATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCAAAGcc   <  1:145184/140‑1 (MQ=255)

                                                                                                                           taagtaagAATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCAAAGCCa  >  1:127153/1‑140 (MQ=255)

GCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGATACTTTCATCTTTTACTTATTCAAAGCCA  >  NC_000913/3172030‑3172292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A