Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	31	34.8	1203930	99.1%	1193094	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,522	C→A	Q248H (CAG→CAT)	eptC ←	phosphoethanolamine transferase EptC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,522	0	C	A	100.0%	120.6 / NA	36	Q248H (CAG→CAT)	eptC	phosphoethanolamine transferase EptC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (20/16); total (20/16)

GTTGCAGGATTTCAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGCTGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATACTG  >  NC_000913/4149386‑4149656

gTTGCAGGATTTCAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATgg                                                                                                                                     >  2:521108/1‑140 (MQ=255)

gTTGCAGGATTTCAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATgg                                                                                                                                     >  1:275678/1‑140 (MQ=255)

  tGCAGGATTTCAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGt                                                                                                                                   <  1:79469/140‑1 (MQ=255)

            cAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCa                                                                                                                         <  2:194016/140‑1 (MQ=255)

                 gtgtACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCAc                                                                                                                    <  2:32610/140‑1 (MQ=255)

                 gtgtACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCAc                                                                                                                    <  2:114913/140‑1 (MQ=255)

                 gtgtACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCAc                                                                                                                    <  1:387662/140‑1 (MQ=255)

                   gtACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCa                                                                                                                  >  2:91777/1‑140 (MQ=255)

                         aCGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTa                                                                                                            >  2:382181/1‑140 (MQ=255)

                              aCGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTg                                                                                                       >  2:562443/1‑140 (MQ=255)

                              aCGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTg                                                                                                       >  2:537795/1‑140 (MQ=255)

                                       cGTTATTGAACGCGGTCAGATTCGGATCTGTTTTATGCAGCGCATCCAGCTCCGGCGGGGTTGCACGCGGATAACCGGACGGTGTGATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCCCTAAAGTGCGCGGTTCg                                                                                              <  2:509221/140‑1 (MQ=255)

                                                 cacGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGAtt                                                                                    >  1:476423/1‑140 (MQ=255)

                                                      tCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCt                                                                               >  2:130620/1‑140 (MQ=255)

                                                      tCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCt                                                                               >  1:167588/1‑140 (MQ=255)

                                                      tCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCt                                                                               >  2:304170/1‑140 (MQ=255)

                                                       cAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCtt                                                                              <  2:414175/140‑1 (MQ=255)

                                                          aTTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGa                                                                           >  1:47053/1‑140 (MQ=255)

                                                                    gTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAg                                                                 >  2:448207/1‑140 (MQ=255)

                                                                    gTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAg                                                                 >  2:504964/1‑140 (MQ=255)

                                                                          tGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTggcgg                                                           >  1:516512/1‑140 (MQ=255)

                                                                           gCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTA‑CCGATTCATCTTTGAAATTAGCCAGTGGCGGCa                                                         <  1:214070/140‑1 (MQ=255)

                                                                                gcATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGc                                                     <  1:278431/140‑1 (MQ=255)

                                                                                 cATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCa                                                    <  1:427611/140‑1 (MQ=255)

                                                                                            ggCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTttcatt                                         >  1:307357/1‑140 (MQ=255)

                                                                                                            gATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGc                         <  2:516512/140‑1 (MQ=255)

                                                                                                            gATAACCGTACAGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGc                         >  2:344998/1‑140 (MQ=255)

                                                                                                                       aGACTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGAGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTg              >  2:317092/1‑140 (MQ=255)

                                                                                                                         aCTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCt            >  2:364493/1‑140 (MQ=255)

                                                                                                                         aCTCATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCt            <  1:130620/140‑1 (MQ=255)

                                                                                                                            cATGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGAc         >  1:492388/1‑140 (MQ=255)

                                                                                                                             aTGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACg        <  2:275678/140‑1 (MQ=255)

                                                                                                                             aTGCGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACg        <  1:562443/140‑1 (MQ=255)

                                                                                                                                cGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATa     <  1:213182/140‑1 (MQ=255)

                                                                                                                                cGTCCGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATa     <  1:387357/140‑1 (MQ=255)

                                                                                                                                cGTCCGCAATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTGCCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATa     <  2:398611/140‑1 (MQ=255)

                                                                                                                                   ccGCGATGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATACTg  >  2:292763/1‑140 (MQ=255)

GTTGCAGGATTTCAATGGTGTACGGACGAGACGTAACTACGTTATTAAACACGGTCAGATTCGGATCGGTTTTATGCAGCGCATCCAGCTCCGGCGTGGTTTCACGCGGATAACCGTACAGACTCATGCGTCCGCGCTGGGTCGACTCGCCAATCACCAGCACTAAAGTGCGCGGTTCGTTACCCGATTCATCTTTGAAATTAGCCAGTGGCGGCAAGGCATTATTTTCATTCAGTAACTTTGTTAGCGAGTTTAGTTGCTGACGATACTG  >  NC_000913/4149386‑4149656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A