Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F73 I1 R1 31 34.8 1203930 99.1% 1193094 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,592,967 C→A S437R (AGC→AGA)  tsr → methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,592,9670CA100.0% 95.4 / NA 28S437R (AGC→AGA) tsrmethyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/13);  total (15/13)

CGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGG  >  NC_000913/4592866‑4593103
                                                                                                     |                                                                                                                                        
cGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGAc                                                                                                    >  2:583090/1‑140 (MQ=255)
      cTGGCCCAGCGCGGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGTCTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGt                                                                                              <  2:274065/140‑1 (MQ=255)
      cTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGt                                                                                              >  2:202057/1‑140 (MQ=255)
       tGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTg                                                                                             >  2:458813/1‑140 (MQ=255)
       tGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTg                                                                                             <  1:583090/140‑1 (MQ=255)
        ggCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGa                                                                                            >  2:170063/1‑140 (MQ=255)
         gCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGAc                                                                                           <  1:224905/140‑1 (MQ=255)
          cccAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACg                                                                                          <  2:249684/140‑1 (MQ=255)
               cgcagcgcCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACAt                                                                                     >  1:220197/1‑140 (MQ=255)
                         aGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGaa                                                                           >  1:34759/1‑140 (MQ=255)
                               cTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgct                                                                     <  2:102567/140‑1 (MQ=255)
                                 cGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgcttc                                                                   <  1:438430/140‑1 (MQ=255)
                                     aaaTTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCAGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATtgcttctgct                                                               >  1:269820/1‑140 (MQ=255)
                                         tAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGcttctgcttctg                                                           <  1:126632/140‑1 (MQ=255)
                                              gCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAg                                                      <  1:218571/140‑1 (MQ=255)
                                                          aCTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGt                                          >  1:18909/1‑140 (MQ=255)
                                                              ggTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAg                                                      <  1:301759/124‑1 (MQ=255)
                                                              ggTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAg                                                      >  2:301759/1‑124 (MQ=255)
                                                                tGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGAt                                    <  2:18909/140‑1 (MQ=255)
                                                                          ggATGTTGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCt                          >  1:180165/1‑140 (MQ=255)
                                                                               ttGGCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCg                     <  1:202057/140‑1 (MQ=255)
                                                                                 ggCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGt                   <  2:220197/140‑1 (MQ=255)
                                                                                  gCTCTACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGtt                  >  2:453981/1‑140 (MQ=255)
                                                                                   ctctACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGGGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGGATCGATCAGGTTGGCTTAGCGGTTg                 >  2:490209/1‑140 (MQ=255)
                                                                                      tACGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTg              >  2:542451/1‑140 (MQ=255)
                                                                                        cGCTGGTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTgag            >  1:285775/1‑140 (MQ=255)
                                                                                             gTCGAAAGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGa       >  2:389478/1‑140 (MQ=255)
                                                                                                  aaGAGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCggg  <  2:314076/140‑1 (MQ=255)
                                                                                                     |                                                                                                                                        
CGTAATCTGGCCCAGCGCAGCGCCCAGGCGGCTCGTGAAATTAAAAGCCTGATTGAAGACTCGGTGGGGAAAGTGGATGTTGGCTCTACGCTGGTCGAAAGCGCCGGGGAAACAATGGCGGAGATTGTCAGCGCCGTGACCCGCGTGACGGACATTATGGGCGAAATTGCTTCTGCTTCTGATGAGCAGAGCCGTGGTATCGATCAGGTTGGCTTAGCGGTTGCTGAGATGGACCGGG  >  NC_000913/4592866‑4593103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A