Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F73 I1 R1	26	44.8	1518598	99.2%	1506449	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,567,832	C→A	S92S (TCG→TCT)	eutH ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,567,832	0	C	A	100.0%	95.7 / NA	28	S92S (TCG→TCT)	eutH	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/11); total (17/11)

GTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTC  >  NC_000913/2567707‑2567960

gTTGGCCCCATCATCGACCCGTGAATTAACCCAGAGTTTACCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGcc                                                                                                                    <  2:732058/140‑1 (MQ=255)

   ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGGGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 <  1:602984/140‑1 (MQ=255)

   ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 >  1:102132/1‑140 (MQ=255)

   ggCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAg                                                                                                                 >  1:647595/1‑140 (MQ=255)

        catcatCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCAttt                                                                                                            >  1:317044/1‑140 (MQ=255)

         atcatcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTc                                                                                                           >  2:193284/1‑140 (MQ=255)

            atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTa                                                                                                        <  2:400200/140‑1 (MQ=255)

            atcGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTa                                                                                                        <  2:102132/140‑1 (MQ=255)

                          tAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTa                                                                                          <  2:317044/140‑1 (MQ=255)

                            aCCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTAcc                                                                                        >  1:554894/1‑140 (MQ=255)

                                   gTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCga                                                                                 >  2:256816/1‑140 (MQ=255)

                                       aGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGccc                                                                                   >  2:599796/1‑134 (MQ=255)

                                       aGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGccc                                                                                   <  1:599796/134‑1 (MQ=255)

                                            cgcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAg                                                                        >  1:121517/1‑140 (MQ=255)

                                            cgcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAg                                                                        >  1:127387/1‑140 (MQ=255)

                                             gcgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGc                                                                       <  2:647595/140‑1 (MQ=255)

                                              cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                                                                            >  1:457429/1‑134 (MQ=255)

                                              cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACg                                                                            <  2:457429/134‑1 (MQ=255)

                                              cgGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGcc                                                                      >  1:729942/1‑140 (MQ=255)

                                                   aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTAc                                                                 <  2:121517/140‑1 (MQ=255)

                                                   aCGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTAc                                                                 <  1:193284/140‑1 (MQ=255)

                                                              cgccAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAgcg                                                      >  1:504709/1‑140 (MQ=255)

                                                                           gCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGAc                                         <  2:127387/140‑1 (MQ=255)

                                                                              aGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCAt                                      >  2:435826/1‑140 (MQ=255)

                                                                                          cccATATCGCACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAg                          >  1:251674/1‑140 (MQ=255)

                                                                                                   cACGCCAGCAGTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCAt                 >  2:311684/1‑140 (MQ=255)

                                                                                                         agcagTGTTCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCAt           <  1:532815/140‑1 (MQ=255)

                                                                                                                ttCCGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCct    >  1:219169/1‑140 (MQ=255)

                                                                                                                  ccGGCGAACATAGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCcttc  >  2:60144/1‑140 (MQ=255)

GTTGGCCCCATCATCGACCCGAGAATTAACCCAGAGTATAGCCACGCGGCTACGTCGCCGCCCGCCAGCTCTTTGGCGAGGAAGAAGCCGCCCATATCGCACGCCAGCAGTGTTCCGGCGAACATCGATGGGTTAGCGCCGAGCATTTCGTAAACCGGAATAATTACCGGCCCGAGAACGTGAGCCAGTACCGGTGCCAGCGCGGTCATACCGACCATCGCCAGGCCCAGTGCGCCCATTGCCATAAAGCCTTC  >  NC_000913/2567707‑2567960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A