Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 146 75.3 2562504 98.6% 2526628 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,169,857 G→A M10I (ATG→ATA)  qseB → DNA‑binding transcriptional activator QseB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,169,8570GA100.0% 303.1 / NA 85M10I (ATG→ATA) qseBDNA‑binding transcriptional activator QseB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/43);  total (42/43)

AGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAC  >  NC_000913/3169722‑3169985
                                                                                                                                       |                                                                                                                                
aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:38130/140‑1 (MQ=255)
aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              >  2:853915/1‑140 (MQ=255)
aGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:940727/140‑1 (MQ=255)
  tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:1141701/1‑140 (MQ=255)
  tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:652609/1‑140 (MQ=255)
  tCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGAtt                                                                                                                            >  1:516717/1‑140 (MQ=255)
    cTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTgg                                                                                                                          >  1:549567/1‑140 (MQ=255)
       aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              <  1:553569/133‑1 (MQ=255)
       aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGa                                                                                                                              >  2:553569/1‑133 (MQ=255)
       aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGa                                                                                                                       >  2:1087176/1‑140 (MQ=255)
       aacaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGa                                                                                                                       >  1:120155/1‑140 (MQ=255)
        acaacTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGAc                                                                                                                      <  2:1104702/140‑1 (MQ=255)
            cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  2:478944/1‑140 (MQ=255)
            cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  1:1251118/1‑140 (MQ=255)
            cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  2:798536/1‑140 (MQ=255)
            cttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCa                                                                                                                  >  1:569619/1‑140 (MQ=255)
             ttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCAt                                                                                                                 <  1:478944/140‑1 (MQ=255)
             ttcttAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCAt                                                                                                                 <  2:93073/140‑1 (MQ=255)
                       aaaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGG‑TTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGc                                                                                                       <  2:223046/139‑1 (MQ=255)
                       aaaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGG‑TTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGc                                                                                                       >  1:223046/1‑139 (MQ=255)
                        aaaaaaTAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTCATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGcc                                                                                                      <  2:1141701/140‑1 (MQ=255)
                          aaaataaaatTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCtt                                                                                                    <  2:217987/140‑1 (MQ=255)
                                aaatTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTaaa                                                                                              <  2:59727/140‑1 (MQ=255)
                                            gTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGc                                                                                  <  1:928690/140‑1 (MQ=255)
                                            gTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGc                                                                                  <  1:853915/140‑1 (MQ=255)
                                                 gagCGCGTTACAACACGGTTTTCTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:640195/140‑1 (MQ=255)
                                                 gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:288968/140‑1 (MQ=255)
                                                 gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:768761/140‑1 (MQ=255)
                                                 gagCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATAACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:681730/1‑140 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATg                                                                                           >  2:1029521/1‑125 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATg                                                                                           <  1:1029521/125‑1 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  1:570313/1‑139 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  2:570313/139‑1 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:1227767/139‑1 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:1227767/1‑139 (MQ=255)
                                                  agCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            >  2:703059/1‑140 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATGGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:640539/137‑1 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATGGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:640539/1‑137 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:288051/1‑137 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:288051/137‑1 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            >  1:1273383/1‑138 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGAc                                                                            <  2:1273383/138‑1 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTg                                                                          >  1:489202/1‑140 (MQ=255)
                                                    cgcgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTg                                                                          >  1:246625/1‑140 (MQ=255)
                                                      cgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGt                                                                        <  2:652609/140‑1 (MQ=255)
                                                      cgTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGt                                                                        <  2:1024432/140‑1 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             <  1:392109/134‑1 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGa                                                                             >  2:392109/1‑134 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  2:100201/1‑140 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  1:364347/1‑140 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  2:855579/1‑140 (MQ=255)
                                                       gTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGtt                                                                       >  1:926003/1‑140 (MQ=255)
                                                               acGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGAAGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAg                                                               <  2:569619/140‑1 (MQ=255)
                                                                   tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  2:584100/1‑140 (MQ=255)
                                                                   tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           <  2:278503/140‑1 (MQ=255)
                                                                   tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  2:640019/1‑140 (MQ=255)
                                                                   tttACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcg                                                           >  1:278503/1‑140 (MQ=255)
                                                                     tACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGgtcgtc                                                         >  1:499522/1‑140 (MQ=255)
                                                                         ggCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAggg                                                     >  1:391934/1‑140 (MQ=255)
                                                                             gcaAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGaaaa                                                 >  1:290870/1‑140 (MQ=255)
                                                                              caAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAag                                                <  1:1087176/140‑1 (MQ=255)
                                                                                aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:798536/140‑1 (MQ=255)
                                                                                aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:703059/140‑1 (MQ=255)
                                                                                aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  2:549567/140‑1 (MQ=255)
                                                                                aaTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  1:1261309/140‑1 (MQ=255)
                                                                                 atatggTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAgg                                             >  1:945624/5‑140 (MQ=255)
                                                                                 aTACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAgg                                             >  1:1181546/1‑140 (MQ=255)
                                                                                        tATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTta                                      >  2:226105/1‑140 (MQ=255)
                                                                                         aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              <  2:315591/131‑1 (MQ=255)
                                                                                         aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAagag                                              >  1:315591/1‑131 (MQ=255)
                                                                                         aTCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTtat                                     >  1:107553/1‑140 (MQ=255)
                                                                                           cGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAg                                   <  2:107553/140‑1 (MQ=255)
                                                                                            gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTTGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:100201/140‑1 (MQ=255)
                                                                                            gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  >  2:408803/1‑140 (MQ=255)
                                                                                            gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:854879/140‑1 (MQ=255)
                                                                                            gCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgc                                  <  1:444689/140‑1 (MQ=255)
                                                                                             cAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAgcg                                 <  1:855579/140‑1 (MQ=255)
                                                                                               gggATGAAAAAATGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCa                               >  1:994831/1‑140 (MQ=255)
                                                                                                 gATGAAAAAATGCGAATTTTACTGATAGAAGATGGCATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCATCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCAcc                             <  2:120155/140‑1 (MQ=255)
                                                                                                     aaaaaaTGCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTat                         >  2:681312/1‑140 (MQ=255)
                                                                                                            gCGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCgg                  <  2:499522/140‑1 (MQ=255)
                                                                                                             cGAATTTTACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGt                 <  1:408803/140‑1 (MQ=255)
                                                                                                                  tttACTGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATcc            <  2:246625/140‑1 (MQ=255)
                                                                                                                       tGATAGAAGATGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGAt       <  1:640019/140‑1 (MQ=255)
                                                                                                                            gaagaTGACATACTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAc  <  1:584100/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                
AGTCCTTAACAACTTCTTAAGGGAAAAAAATAAAATTTAGTGCTGTACAGAGCGCGTTACAACACGGTTTACTGGCAGCAAATACGGTTATCGCAGGGATGAAAAAATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGTCGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAAC  >  NC_000913/3169722‑3169985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A