Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F28 I1 R1 146 75.3 2562504 98.6% 2526628 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,657,230 T→C V79A (GTA→GCA)  hdeD → acid‑resistance membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,2300TC100.0% 295.7 / NA 79V79A (GTA→GCA) hdeDacid‑resistance membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/35);  total (44/35)

GTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATG  >  NC_000913/3657094‑3657366
                                                                                                                                        |                                                                                                                                        
gtcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatt                                                                                                                                       <  1:1048805/140‑1 (MQ=255)
 tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  2:553358/1‑140 (MQ=255)
 tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  1:214396/1‑140 (MQ=255)
 tcgGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGcatta                                                                                                                                      >  1:547084/1‑140 (MQ=255)
    ggTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATcc                                                                                                                                   >  2:611128/1‑140 (MQ=255)
      ttGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCgg                                                                                                                                 >  1:1030580/1‑140 (MQ=255)
       tGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGt                                                                                                                                <  2:755459/140‑1 (MQ=255)
          tgtgTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTc                                                                                                                             <  2:1109620/140‑1 (MQ=255)
              tATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCtcg                                                                                                                         <  2:685752/140‑1 (MQ=255)
                 cAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCtcgtcg                                                                                                                      <  2:1132579/140‑1 (MQ=255)
                   gTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCa                                                                                                                    >  1:881532/1‑140 (MQ=255)
                   gTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCa                                                                                                                    <  1:611128/140‑1 (MQ=255)
                     ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTCGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCTCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:881532/140‑1 (MQ=255)
                     ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:1030580/140‑1 (MQ=255)
                     ttCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  2:805463/140‑1 (MQ=255)
                       cccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCg                                                                                                                <  1:294567/140‑1 (MQ=255)
                        ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  1:39889/140‑1 (MQ=255)
                        ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  1:716853/140‑1 (MQ=255)
                        ccGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGc                                                                                                               <  2:775336/140‑1 (MQ=255)
                              gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  <  1:392012/131‑1 (MQ=255)
                              gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGt                                                                                                                  >  2:392012/1‑131 (MQ=255)
                              gTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCtattt                                                                                                         <  2:799663/140‑1 (MQ=255)
                                         tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  2:192307/140‑1 (MQ=255)
                                         tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  1:1143457/140‑1 (MQ=255)
                                         tatTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCt                                                                                              <  2:1227640/140‑1 (MQ=255)
                                          atTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTa                                                                                             >  2:961972/1‑140 (MQ=255)
                                              tAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATttc                                                                                         <  1:553358/140‑1 (MQ=255)
                                                     agtagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGACGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATcc                                                                                  >  1:682637/1‑140 (MQ=255)
                                                      gtagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 <  1:786739/140‑1 (MQ=255)
                                                       tagtGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgc                                                                                >  1:843322/1‑140 (MQ=255)
                                                            ggTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCAt                                                                                    >  1:78239/1‑131 (MQ=255)
                                                            ggTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCAt                                                                                    <  2:78239/131‑1 (MQ=255)
                                                               gCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGa                                                                        >  1:7315/1‑140 (MQ=255)
                                                               gCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGa                                                                        >  2:433947/1‑140 (MQ=255)
                                                                 attattAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGc                                                                      >  1:588412/1‑140 (MQ=255)
                                                                   tattaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 >  2:965987/1‑127 (MQ=255)
                                                                   tattaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcg                                                                                 <  1:965987/127‑1 (MQ=255)
                                                                    attaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  1:100743/1‑140 (MQ=255)
                                                                    attaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  2:599517/1‑140 (MQ=255)
                                                                     ttaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgcg                                                                               <  1:67712/127‑1 (MQ=255)
                                                                     ttaATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCcgcg                                                                               >  2:67712/1‑127 (MQ=255)
                                                                      taATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGc                                                                 >  1:768091/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGTCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:1117593/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:807507/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:999917/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:662792/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:573532/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:330263/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:9076/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  2:335553/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:1147837/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:696861/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:765112/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:949062/1‑140 (MQ=255)
                                                                             cTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGACTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTg                                                          >  1:225568/1‑140 (MQ=255)
                                                                               cGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGcc                                                        >  1:1164537/1‑140 (MQ=255)
                                                                                     ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   <  2:268815/123‑1 (MQ=255)
                                                                                     ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTgg                                                                   >  1:268815/1‑123 (MQ=255)
                                                                                     ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCg                                                  >  2:901344/1‑140 (MQ=255)
                                                                                     ttGCGCTTATTGTCGGGTTATTCAGCAACCGCAGGCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCg                                                  >  1:200099/1‑140 (MQ=255)
                                                                                       gcgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGc                                                >  2:1036341/1‑140 (MQ=255)
                                                                                        cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCa                                                       >  1:406582/1‑132 (MQ=255)
                                                                                        cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCa                                                       <  2:406582/132‑1 (MQ=255)
                                                                                        cgcTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCa                                               >  2:471517/1‑140 (MQ=255)
                                                                                          cTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCAtt                                             >  2:1193877/1‑140 (MQ=255)
                                                                                             aTTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTAt                                          >  1:465381/1‑140 (MQ=255)
                                                                                               tGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTg                                        <  1:433947/140‑1 (MQ=255)
                                                                                                  cGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCg                                     >  1:930668/1‑140 (MQ=255)
                                                                                                    ggTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   <  2:843322/140‑1 (MQ=255)
                                                                                                        aTTCAGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGt                               >  2:145878/1‑140 (MQ=255)
                                                                                                            aGCAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCtg                           <  1:662792/140‑1 (MQ=255)
                                                                                                              cAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   <  2:803438/130‑1 (MQ=255)
                                                                                                              cAACCGCAGTCATAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGt                                   >  1:803438/1‑130 (MQ=255)
                                                                                                                     aGTCATAATTTCTGGCCGGCATTATCCGGTTTTCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAgg                  <  2:353164/140‑1 (MQ=255)
                                                                                                                          tAATTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAAGGGTTa             <  2:588412/140‑1 (MQ=255)
                                                                                                                            aTTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTAtt           <  2:1096018/140‑1 (MQ=255)
                                                                                                                            aTTTCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTAtt           <  2:100743/140‑1 (MQ=255)
                                                                                                                             tttCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTc          <  2:465381/140‑1 (MQ=255)
                                                                                                                               tCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGc        <  2:765112/140‑1 (MQ=255)
                                                                                                                               tCTGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGc        >  2:266643/1‑140 (MQ=255)
                                                                                                                                 tGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCt      >  2:797270/1‑140 (MQ=255)
                                                                                                                                 tGGCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCt      >  1:1253266/1‑140 (MQ=255)
                                                                                                                                   gCCGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGACTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCtga    <  2:225568/140‑1 (MQ=255)
                                                                                                                                     cGGCATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCtgatg  <  1:469795/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTCGGGTTGCTGTGTATCAGTTTCCCGTTCGTCTCTGGCGATATTTTAAGCACAGTAGTGGGTGCATTATTAATCTGCTCGGGTATTGCGCTTATTGTCGGGTTATTCAGCAACCGCAGTCATAATTTCTGGCCGGTATTATCCGGTTTCCTCGTCGCAGTCGCCTATTTATTGATCGGCTATTTCTTCATCCGCGCACCGGAGCTGGGCATTTTTGCCATTGCGGCATTTATTGCCGGTTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATG  >  NC_000913/3657094‑3657366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A