Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	146	75.3	2562504	98.6%	2526628	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,928	A→G	L569P (CTG→CCG)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,928	0	A	G	100.0%	179.7 / NA	49	L569P (CTG→CCG)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/27); total (22/27)

TGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGT  >  NC_000913/3923794‑3924064

tGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCggg                                                                                                                                        >  1:718825/1‑137 (MQ=255)

tGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGgtg                                                                                                                                     >  2:951464/1‑140 (MQ=255)

   agaTGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCTATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTtc                                                                                                                                  >  2:56891/1‑140 (MQ=255)

       ggCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGt                                                                                                                              <  1:951464/140‑1 (MQ=255)

        gCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGtt                                                                                                                             >  2:670588/1‑140 (MQ=255)

         ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCTATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:56891/140‑1 (MQ=255)

         ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:389021/140‑1 (MQ=255)

         ccGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTa                                                                                                                            <  1:464026/140‑1 (MQ=255)

          cGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTAc                                                                                                                           >  2:741572/1‑140 (MQ=255)

               gCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgc                                                                                                                      >  2:914191/1‑140 (MQ=255)

                  tGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgctgc                                                                                                                   >  2:145048/1‑140 (MQ=255)

                  tGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAgctgc                                                                                                                   >  1:939280/1‑140 (MQ=255)

                    aCGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCAATTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCtt                                                                                                                 >  2:1191403/1‑140 (MQ=255)

                         aGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGa                                                                                                            <  1:1148405/140‑1 (MQ=255)

                         aGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGa                                                                                                            >  2:724885/1‑140 (MQ=255)

                          gAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAt                                                                                                           <  2:528909/140‑1 (MQ=255)

                            aaTACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:724885/140‑1 (MQ=255)

                            aaTACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:914191/140‑1 (MQ=255)

                                 gcgAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCt                                                                                                    >  2:455732/1‑140 (MQ=255)

                                   gAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTg                                                                                                  <  1:884424/140‑1 (MQ=255)

                                      gCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTg                                                                                               <  1:670588/140‑1 (MQ=255)

                                          ggCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgc                                                                                           <  2:611587/140‑1 (MQ=255)

                                           gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         <  1:21781/125‑1 (MQ=255)

                                           gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGAtct                                                                                                         >  2:21781/1‑125 (MQ=255)

                                           gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          >  2:756276/1‑140 (MQ=255)

                                           gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          <  2:390986/140‑1 (MQ=255)

                                           gCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGgcgcg                                                                                          <  1:741572/140‑1 (MQ=255)

                                                tAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGata                                                                                     <  1:356474/140‑1 (MQ=255)

                                                 agagGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGatat                                                                                    <  1:1191403/140‑1 (MQ=255)

                                                         gTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCg                                                                            >  1:1208006/1‑140 (MQ=255)

                                                          tttGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGt                                                                           >  1:708917/1‑140 (MQ=255)

                                                                   gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:360955/140‑1 (MQ=255)

                                                                   gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:756276/140‑1 (MQ=255)

                                                                   gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  1:264733/140‑1 (MQ=255)

                                                                   gTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCt                                                                  <  2:667289/140‑1 (MQ=255)

                                                                        gTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTGCGCAGCTGCTTTTCGATCTCATCTTGCTGGAGCGCGATATAACCTTCGTATTTAACCTGAAtc                                                             <  2:512554/140‑1 (MQ=255)

                                                                        gTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAAtc                                                             <  1:886379/140‑1 (MQ=255)

                                                                           tGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCa                                                          <  1:363120/140‑1 (MQ=255)

                                                                           tGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCa                                                          <  1:455732/140‑1 (MQ=255)

                                                                            ggCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCaa                                                         >  2:1209034/1‑140 (MQ=255)

                                                                                             aaaGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGt                                        >  1:1173899/1‑140 (MQ=255)

                                                                                               aGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTc                                      >  2:971278/1‑140 (MQ=255)

                                                                                                 aCCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAACCTCAACCTGTTCCGCCGCCTGTTCGt                                    <  2:1208006/140‑1 (MQ=255)

                                                                                                     gATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGt                                <  2:708917/140‑1 (MQ=255)

                                                                                                     gATACCTGGCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGt                                <  2:598045/140‑1 (MQ=255)

                                                                                                             gCGGTAATCCAGTGTCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAg                        >  2:698469/1‑140 (MQ=255)

                                                                                                                         tgtCGCGGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCg            >  2:232163/1‑140 (MQ=255)

                                                                                                                              cgGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGc       >  2:1199663/1‑140 (MQ=255)

                                                                                                                              cgGGTAGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGc       >  1:594178/1‑140 (MQ=255)

                                                                                                                                   aGCGGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGt  >  1:1112198/1‑140 (MQ=255)

TGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTAAGTTTGGCGATCACTTCGTTAGAAAGACCGGATACCTGGCGGTAATCCAGTGTCGCGGGTAGCAGGGTGTTCTCGTTACGCAGCTGCTTTTCGATCTCATCTTGCTGGCGCGCGATATAACCTTCGTATTTAACCTGAATCTCAACCTGTTCCGCCGCCTGTTCGTCTGTCAACGCAGGGGCAAACGGCGTCAGCGTGGT  >  NC_000913/3923794‑3924064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A