Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,736,296	G→A	A51T (GCA→ACA)	bamD →	outer membrane protein assembly factor BamD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,736,296	0	G	A	100.0%	190.0 / NA	54	A51T (GCA→ACA)	bamD	outer membrane protein assembly factor BamD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/30); total (24/30)

TCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAT  >  NC_000913/2736163‑2736432

tCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGGCTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATaa                                                                                                                                    >  1:276300/1‑140 (MQ=255)

 cTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAAc                                                                                                                                   <  2:432057/140‑1 (MQ=255)

   ggtggCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGc                                                                                                                                 <  2:835586/140‑1 (MQ=255)

        cAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTg                                                                                                                            <  1:197071/140‑1 (MQ=255)

         aGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTgg                                                                                                                           <  2:52531/140‑1 (MQ=255)

         aGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTgg                                                                                                                           <  1:381231/140‑1 (MQ=255)

          gccgccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGa                                                                                                                          >  1:902175/1‑140 (MQ=255)

             gccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGc                                                                                                                       <  2:902175/140‑1 (MQ=255)

              ccACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCg                                                                                                                      <  1:423682/140‑1 (MQ=255)

                        ccTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATc                                                                                                            >  1:4443/1‑140 (MQ=255)

                                 ggCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGGCAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGt                                                                                                   >  2:945293/1‑140 (MQ=255)

                                  gCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGtt                                                                                                  <  1:384768/140‑1 (MQ=255)

                                  gCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGtt                                                                                                  <  1:945514/140‑1 (MQ=255)

                                      gTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGt                                                                                              >  1:247263/1‑140 (MQ=255)

                                              gggTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTc                                                                                      <  1:719963/140‑1 (MQ=255)

                                                 tCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgca                                                                                   >  2:873352/1‑140 (MQ=255)

                                                 tCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgca                                                                                   <  1:777744/140‑1 (MQ=255)

                                                    aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                                                                                >  2:828661/1‑140 (MQ=255)

                                                    aaGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCgcagca                                                                                >  1:673069/1‑140 (MQ=255)

                                                      ggAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAgg                                                                              <  1:873352/140‑1 (MQ=255)

                                                      ggAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAgg                                                                              <  2:32352/140‑1 (MQ=255)

                                                        aagaagTACCTGATAAACCGTCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAAGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTg                                                                            <  2:276300/140‑1 (MQ=255)

                                                         agaagTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGc                                                                           >  2:456019/1‑140 (MQ=255)

                                                                 cTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        >  2:582551/1‑135 (MQ=255)

                                                                 cTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        <  1:582551/135‑1 (MQ=255)

                                                                  tGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc                                                                  >  2:551448/1‑140 (MQ=255)

                                                                  tGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtc                                                                  <  2:4443/140‑1 (MQ=255)

                                                                     tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               >  2:207437/1‑140 (MQ=255)

                                                                     tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               <  2:247263/140‑1 (MQ=255)

                                                                     tAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCa                                                               >  2:706477/1‑140 (MQ=255)

                                                                        tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtct                                                                 <  2:974712/135‑1 (MQ=255)

                                                                        tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGAtct                                                                 >  1:974712/1‑135 (MQ=255)

                                                                        tCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCt                                                            >  1:168410/1‑140 (MQ=255)

                                                                          cGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        >  1:253061/1‑126 (MQ=255)

                                                                          cGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGc                                                                        <  2:253061/126‑1 (MQ=255)

                                                                            ccAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGc                                                        <  2:381432/140‑1 (MQ=255)

                                                                             cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  2:673069/140‑1 (MQ=255)

                                                                             cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  1:393348/140‑1 (MQ=255)

                                                                             cAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGcc                                                       <  1:551448/140‑1 (MQ=255)

                                                                                              aCTGCACTACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGa                                      >  2:341867/1‑140 (MQ=255)

                                                                                               cTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGAt                                     <  1:259889/140‑1 (MQ=255)

                                                                                                tGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGAtt                                    <  1:706477/140‑1 (MQ=255)

                                                                                                 gCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGACCTCATCTACGCCTACTATAAAAACGCCGAttt                                   <  2:843999/140‑1 (MQ=255)

                                                                                                          aaGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGc                          >  1:923243/1‑140 (MQ=255)

                                                                                                           aGCTGCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGca                         <  1:828661/140‑1 (MQ=255)

                                                                                                               gCAGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAgg                     >  2:884785/1‑140 (MQ=255)

                                                                                                                 aGGACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCt                   >  2:698855/1‑140 (MQ=255)

                                                                                                                   gACGGTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGc                 <  1:25893/140‑1 (MQ=255)

                                                                                                                      ggTAACTGGAGACAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCat              >  2:148435/1‑140 (MQ=255)

                                                                                                                                 aCAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTa   >  1:920324/1‑140 (MQ=255)

                                                                                                                                  cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCatcgat          <  2:765497/132‑1 (MQ=255)

                                                                                                                                  cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  <  1:772704/140‑1 (MQ=255)

                                                                                                                                  cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  >  1:118853/1‑140 (MQ=255)

                                                                                                                                  cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  <  1:341867/140‑1 (MQ=255)

                                                                                                                                  cAGACAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAt  >  2:24437/1‑140 (MQ=255)

TCTGGTGGCAGCCGCCACACTAAGCCTGTTTTTGGCGGGTTGCTCGGGGTCAAAGGAAGAAGTACCTGATAATCCGCCAAATGAAATTTACGCGACTGCACAACAAAAGCTGCAGGACGGTAACTGGAGACAGGCAATAACGCAACTGGAAGCGTTAGATAATCGCTATCCGTTTGGTCCGTATTCGCAGCAGGTGCAGCTGGATCTCATCTACGCCTACTATAAAAACGCCGATTTGCCGTTAGCACAGGCTGCCATCGATCGTTTTAT  >  NC_000913/2736163‑2736432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A