Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,907,474	A→G	G156G (GGT→GGC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,474	0	A	G	100.0%	163.1 / NA	46	G156G (GGT→GGC)	eno	enolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/27); total (19/27)

CTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCA  >  NC_000913/2907341‑2907607

cTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtg                                                                                                                                 <  1:906142/140‑1 (MQ=255)

 tttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtga                                                                                                                                <  1:1006156/140‑1 (MQ=255)

 tttCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtga                                                                                                                                >  2:242109/1‑140 (MQ=255)

   tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  2:912196/1‑140 (MQ=255)

   tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  2:572561/1‑140 (MQ=255)

   tCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTtgatg                                                                                                                              >  1:546699/1‑140 (MQ=255)

         tCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGTGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTtc                                                                                                                        <  2:480467/140‑1 (MQ=255)

          cAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATACCAACGTTGTTGTCAGCGTGCTAACCGCCGTTGATGATGTtca                                                                                                                       <  2:280378/140‑1 (MQ=255)

              aCTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTcatcat                                                                                                                   <  1:34850/140‑1 (MQ=255)

                   tGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGaa                                                                                                              <  1:19827/140‑1 (MQ=255)

                   tGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGaa                                                                                                              <  1:637464/140‑1 (MQ=255)

                     ccAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAAcc                                                                                                            >  2:618820/1‑140 (MQ=255)

                         gTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCa                                                                                                        <  2:463059/140‑1 (MQ=255)

                            aTGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATag                                                                                                     <  1:912196/140‑1 (MQ=255)

                                    cTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTg                                                                                             >  1:123569/1‑140 (MQ=255)

                                      tCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGcc                                                                                           <  1:571693/140‑1 (MQ=255)

                                      tCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGcc                                                                                           <  1:174009/140‑1 (MQ=255)

                                             ccATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTa                                                                                    <  1:552007/140‑1 (MQ=255)

                                             ccATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTa                                                                                    <  2:940493/140‑1 (MQ=255)

                                              cATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTAc                                                                                   >  1:330761/1‑140 (MQ=255)

                                                  cGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgtt                                                                               <  2:645340/140‑1 (MQ=255)

                                                         cttcttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCgttcagttca                                                                        >  2:680509/1‑140 (MQ=255)

                                                            cttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCg                                                                     <  1:572561/140‑1 (MQ=255)

                                                            cttTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCg                                                                     <  1:185785/140‑1 (MQ=255)

                                                             tttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGa                                                                    <  1:618820/140‑1 (MQ=255)

                                                             tttCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGa                                                                    <  1:140193/140‑1 (MQ=255)

                                                               tCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGAtg                                                                  <  2:514462/140‑1 (MQ=255)

                                                                     ttttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCg                                                            >  2:464493/1‑140 (MQ=255)

                                                                       ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  2:972940/1‑140 (MQ=255)

                                                                       ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  1:548909/1‑140 (MQ=255)

                                                                       ttCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTa                                                          >  2:14786/1‑140 (MQ=255)

                                                                               aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa                                                  <  1:972940/140‑1 (MQ=255)

                                                                               aaCCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCa                                                  <  1:680509/140‑1 (MQ=255)

                                                                                          tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATa                                                <  1:202736/131‑1 (MQ=255)

                                                                                          tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATa                                                >  2:202736/1‑131 (MQ=255)

                                                                                          tCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTagca                                       <  2:546699/140‑1 (MQ=255)

                                                                                              gAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTg                                   >  2:558281/1‑140 (MQ=255)

                                                                                                 ttCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTgcag                                <  1:14786/140‑1 (MQ=255)

                                                                                                         tatCAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTg                        >  1:271725/1‑140 (MQ=255)

                                                                                                            cAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                     >  1:23119/1‑140 (MQ=255)

                                                                                                            cAACGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCg                     >  2:173552/1‑140 (MQ=255)

                                                                                                              aCGTTGTTGTCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGtt                   <  2:23119/140‑1 (MQ=255)

                                                                                                                      gtCAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCagag           >  2:651159/1‑140 (MQ=255)

                                                                                                                        cAGCGTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGAt         <  2:605215/140‑1 (MQ=255)

                                                                                                                            gTGCTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAg     <  1:242109/140‑1 (MQ=255)

                                                                                                                               cTCACCGCCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCa  >  1:236767/1‑140 (MQ=255)

CTTTCGCTTTCAGAACTTTTGCCAGGTGATGGAAAACTTCAGAACCCATGCGGATGGCTTCTTTCACAGTTTTCGCGCCAACCGGCTGAATCATGAATTCCTGGATATCAACGTTGTTGTCAGCGTGCTCACCACCGTTGATGATGTTCATCATCGGAACCGGCATAGAGTATTTGCCCGGAGTACCGTTCAGTTCAGCGATGTGCTCGTACAGCGGCATACCTTTAGCAGCTGCAGCAGCTTTGGCGTTAGCCAGAGATACAGCCA  >  NC_000913/2907341‑2907607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A