Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	153	60.1	2040486	99.3%	2026202	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,846,404	A→G	V255A (GTG→GCG)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,846,404	0	A	G	100.0%	163.2 / NA	46	V255A (GTG→GCG)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/25); total (21/25)

CCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAG  >  NC_000913/3846269‑3846540

cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:104214/140‑1 (MQ=255)

cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:676459/140‑1 (MQ=255)

cccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATa                                                                                                                                      <  1:695061/140‑1 (MQ=255)

 ccTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATAc                                                                                                                                     <  2:191435/140‑1 (MQ=255)

   tGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTc                                                                                                                                   >  2:293647/1‑140 (MQ=255)

     aaTCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTcaa                                                                                                                                 >  1:772776/1‑140 (MQ=255)

            aCCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGAt                                                                                                                          <  1:398088/140‑1 (MQ=255)

                   tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:293647/140‑1 (MQ=255)

                   tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:485779/140‑1 (MQ=255)

                   tAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCAc                                                                                                                   <  1:809404/140‑1 (MQ=255)

                            tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCata                                                                                                          <  2:772776/140‑1 (MQ=255)

                            tCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCata                                                                                                          <  2:434842/140‑1 (MQ=255)

                                    tGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  <  1:342292/140‑1 (MQ=255)

                                                    ttGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGGCTCTTTGTcc                                                                                  >  1:699281/1‑140 (MQ=255)

                                                     tGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 <  1:974756/140‑1 (MQ=255)

                                                      gACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  >  2:89461/1‑122 (MQ=255)

                                                      gACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGa                                                                                                  <  1:89461/122‑1 (MQ=255)

                                                         cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 <  1:675107/136‑1 (MQ=255)

                                                         cACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCct                                                                                 >  2:675107/1‑136 (MQ=255)

                                                             ggTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTg                                                                         >  2:843562/1‑140 (MQ=255)

                                                              gTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGa                                                                        >  1:919846/1‑140 (MQ=255)

                                                                cGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGAtc                                                                      >  2:838733/1‑140 (MQ=255)

                                                                        aTACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGcc                                                              >  2:788552/1‑140 (MQ=255)

                                                                            gTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAAc                                                          <  2:407792/140‑1 (MQ=255)

                                                                             tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  1:860470/1‑140 (MQ=255)

                                                                             tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  2:530105/1‑140 (MQ=255)

                                                                             tACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACa                                                         >  1:702302/1‑140 (MQ=255)

                                                                                     agagGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCa                                                 >  1:77699/1‑140 (MQ=255)

                                                                                       agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                               >  2:95472/1‑140 (MQ=255)

                                                                                       agGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGc                                               >  1:227841/1‑140 (MQ=255)

                                                                                            aaTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGc                                          >  2:344039/1‑140 (MQ=255)

                                                                                             aTGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTCTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         <  2:702302/140‑1 (MQ=255)

                                                                                               gTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGa                                       <  2:77699/140‑1 (MQ=255)

                                                                                                 tGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAgg                                     >  1:874465/1‑140 (MQ=255)

                                                                                                      aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         >  2:666384/1‑131 (MQ=255)

                                                                                                      aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGcc                                         <  1:666384/131‑1 (MQ=255)

                                                                                                      aaGCACAGCAGCCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATa                                <  1:95472/140‑1 (MQ=255)

                                                                                                                gcCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                      >  2:657543/1‑140 (MQ=255)

                                                                                                                gcCAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCggg                      <  1:788552/140‑1 (MQ=255)

                                                                                                                 ccAGATCACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCgggg                     <  1:838733/140‑1 (MQ=255)

                                                                                                                       cACTTTGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGGGTCGc               <  2:699281/140‑1 (MQ=255)

                                                                                                                          tttGTTTTTCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGc            >  2:294483/1‑140 (MQ=255)

                                                                                                                                 ttCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACg     >  2:671249/1‑140 (MQ=255)

                                                                                                                                 ttCAGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACg     <  2:256762/140‑1 (MQ=255)

                                                                                                                                    aGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAg  <  1:710234/140‑1 (MQ=255)

                                                                                                                                    aGCGCATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAg  <  2:919846/140‑1 (MQ=255)

CCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTCTTTGTCCTCTTCGCTGATCTCCTCGCCGAACAGTTCTTCAGCTTTGCCGAGGCCATAAGATTCCGGGGAGTCGCTGCCGTAACGCAG  >  NC_000913/3846269‑3846540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A