Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	224	87.5	2960670	99.3%	2939945	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,767,445	(C)_7→8	intergenic (+90/‑265)	rnlB → / → yfjP	CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,767,438	1	.	C	92.9%	160.2 / 5.7	56	intergenic (+83/‑272)	rnlB/yfjP	CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
Reads supporting (aligned to +/- strand): ref base . (4/0); new base C (27/25); total (31/25)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGT  >  NC_000913/2767301‑2767572

aGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑cc                                                                                                                                        >  1:988009/1‑140 (MQ=255)

  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑c                                                                                                                                     <  2:252229/140‑1 (MQ=255)

  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑c                                                                                                                                     >  1:452795/1‑140 (MQ=255)

  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑c                                                                                                                                     <  2:452795/140‑1 (MQ=255)

  ccTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑acc‑c                                                                                                                                     >  1:1141134/1‑140 (MQ=255)

    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccc                                                                                                                                   >  2:710888/1‑140 (MQ=255)

    tctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccc                                                                                                                                   >  2:653291/1‑140 (MQ=255)

     ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc                                                                                                                                       <  2:1377155/136‑1 (MQ=255)

     ctGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc                                                                                                                                       >  1:1377155/1‑136 (MQ=255)

      tGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccccc                                                                                                                                 <  1:349166/140‑2 (MQ=255)

        ttttGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccccctc                                                                                                                               >  2:1174485/1‑137 (MQ=255)

           tGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTcaca                                                                                                                             <  1:1174485/140‑1 (MQ=255)

           tGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTcaca                                                                                                                             <  1:851244/140‑1 (MQ=255)

                cccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTc                                                                                                                        <  2:885794/140‑1 (MQ=255)

                 ccGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTcc                                                                                                                       <  1:710888/140‑1 (MQ=255)

                  cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑‑CCC‑CCCCTCACAACGTCCt                                                                                                                      >  1:419673/1‑140 (MQ=255)

                  cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AACCCCC‑CCCCTCACAACGTcc                                                                                                                       >  1:944502/1‑140 (MQ=255)

                  cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccccc                                                                                                                                 >  2:782831/1‑127 (MQ=255)

                  cGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑ccc‑ccccc                                                                                                                                 <  1:782831/128‑2 (MQ=255)

                             gCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAac                                                                                                           <  2:727610/140‑1 (MQ=255)

                               cAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAacac                                                                                                         >  2:365004/1‑140 (MQ=255)

                                 aGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCTTAGGGAACACGt                                                                                                       >  1:1340674/1‑140 (MQ=255)

                                  gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAac                                                                                                           >  1:1436901/1‑135 (MQ=255)

                                  gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAac                                                                                                           <  2:1436901/135‑1 (MQ=255)

                                  gTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACACCCCGCACATGGAacaagct                                                                                                      >  2:980372/1‑136 (MQ=255)

                                    ccTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGtttt                                                                                                    <  2:861580/140‑1 (MQ=255)

                                       gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCTTAGGGAACACGTTTTccc                                                                                                 <  2:1340674/140‑1 (MQ=255)

                                       gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                                 >  2:460006/1‑140 (MQ=255)

                                       gTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTccc                                                                                                 <  2:1181294/140‑1 (MQ=255)

                                            tGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCtgtg                                                                                            <  1:89607/140‑1 (MQ=255)

                                              gATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCa                                                                                          <  1:460006/140‑1 (MQ=255)

                                                      aaaCTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGAc                                                                                  <  1:551214/140‑1 (MQ=255)

                                                                ttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑ccc                                                                       <  1:653291/140‑1 (MQ=255)

                                                                         gacaggTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTaa                                                              <  1:916798/135‑1 (MQ=255)

                                                                          aCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTaaa                                                             <  2:1184616/140‑1 (MQ=255)

                                                                               tataGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTa                                                               >  1:671584/1‑133 (MQ=255)

                                                                               tataGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTa                                                               <  2:671584/133‑1 (MQ=255)

                                                                                      ttCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCaataat                                                 >  2:917595/1‑140 (MQ=255)

                                                                                       tCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTaaa                                                             <  1:819235/127‑1 (MQ=255)

                                                                                       tCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTaaa                                                             >  2:819235/1‑127 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAAA‑‑CAC‑CCCCTCACAACGTCACGCATAGGGAACACGATTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  2:238145/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACTTGGCGCATAGGGAACAACTTTTCCCCCGCGCAACCCCGACTAACCTG‑CCACCCCCTTAACCTTGTCaagactatc                                              >  2:1000778/1‑133 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATATGGAACACGTTTGCCCCGGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATCCTGTc                                              >  2:916798/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTa                                                               >  2:799134/1‑123 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTa                                                               <  1:799134/123‑1 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  2:86330/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  1:467963/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  1:1241044/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  2:356845/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTc                                              >  1:949359/1‑140 (MQ=255)

                                                                                         gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCGCACAACGTCCTGCATAAGGTACACGAATTCCCCAGCCCACCCACGACTAATTTT‑CCCCGCCTTCACACTTGTCAATAATGTc                                              >  2:828977/1‑140 (MQ=255)

                                                                                                     tGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑CCCACCCCTCACAACGTCCTGCATAGGGTCCACGATTCACACTGTGAGCACACGCCTAAACAT‑CCCCCCCCTTAAACTTCTCAATATTATCACCCATTTTccg                                  >  2:351546/1‑138 (MQ=255)

                                                                                                      gATATTAACTTGTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg                                 <  2:1241044/140‑1 (MQ=255)

                                                                                                                 gTCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACCCGGTTTCCCCTGTCCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGCAATCCTatga                      >  1:1472311/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                     >  2:641917/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                     >  1:114246/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                     >  1:1332532/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                     >  1:49446/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgat                     >  1:1060134/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑‑CCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatgatg                    >  1:1206834/1‑140 (MQ=255)

                                                                                                                  tCCAATATGATCAAATAGCATT‑AA‑‑CCCGCCCCTCATAACGTCCTGCATAGGGAACAAATTTGCCCCTGTGTACCACCGCCTAAATTT‑GCCCCCCTTTAATCTGTTCAATAATGCCACCAATGTCCTGCAAATCCTatgag                     >  2:968419/1‑139 (MQ=255)

                                                                                                                       tatGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCtt                <  1:828977/140‑1 (MQ=255)

                                                                                                                         tGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGACCACGTTTTCCCCTGAGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc              <  2:187213/140‑1 (MQ=255)

                                                                                                                         tGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc              <  1:968419/140‑1 (MQ=255)

                                                                                                                         tGATCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc              <  2:790124/140‑1 (MQ=255)

                                                                                                                           aTCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTccc            >  1:897254/1‑140 (MQ=255)

                                                                                                                            tCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                       <  1:1464244/128‑1 (MQ=255)

                                                                                                                            tCAAATAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTatg                       >  2:1464244/1‑128 (MQ=255)

                                                                                                                            tCAAAAAGCATTAAA‑‑CCC‑CCCCTCCCAACACCCCCCATAGGGAAAAAGTTTTCCCCCCTGCCCCCCCCCCCAAAATT‑TCCCCCCCTTAAACCTCTCAATAAATTCACCCCTTTTCTCGGAATCCTATGATGCCTTTcccc           >  2:947906/1‑139 (MQ=255)

                                                                                                                                aTAGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTTCCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTa        >  1:547890/1‑140 (MQ=255)

                                                                                                                                  aGCATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt     <  2:1332532/140‑1 (MQ=255)

                                                                                                                                    cATT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATgg   >  2:204554/1‑140 (MQ=255)

                                                                                                                                     aTT‑AAC‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGt  <  1:365004/140‑1 (MQ=255)

AGCCTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGAAACTCTTCTTTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATT‑AA‑‑CCC‑CCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAATTT‑CCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGT  >  NC_000913/2767301‑2767572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A