Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F28 I1 R1	224	87.5	2960670	99.3%	2939945	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,190,744	A→G	Y38C (TAC→TGC)	yqiI →	putative fimbrial protein YqiI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,190,744	0	A	G	99.1%	385.4 / ‑4.9	111	Y38C (TAC→TGC)	yqiI	putative fimbrial protein YqiI
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (53/57); total (54/57)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 112 total aligned reads displayed.

TGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTACGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGG  >  NC_000913/3190609‑3190880

     ccAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAg                                                                                                                                  <  1:934594/140‑1 (MQ=255)

      cAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGa                                                                                                                                 <  1:1157712/140‑1 (MQ=255)

       aaCCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGaa                                                                                                                                <  1:1329320/140‑1 (MQ=255)

       aaCCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGaa                                                                                                                                <  1:566233/140‑1 (MQ=255)

            agaTCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATctct                                                                                                                           <  1:399230/140‑1 (MQ=255)

            agaTCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATctct                                                                                                                           <  2:1469770/140‑1 (MQ=255)

              aTCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAg                                                                                                                                  >  1:915154/1‑131 (MQ=255)

              aTCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAg                                                                                                                                  <  2:915154/131‑1 (MQ=255)

               tCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑c                                                                                                                       >  1:95766/1‑140 (MQ=255)

                    aataTGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAg                                                                                                                  >  2:747491/1‑140 (MQ=255)

                    aataTGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAg                                                                                                                  <  1:1117541/140‑1 (MQ=255)

                       atGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGa                                                                                                               >  2:744974/1‑140 (MQ=255)

                       atGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGa                                                                                                               >  2:524820/1‑140 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCg                                                                                                                >  2:591802/1‑138 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCg                                                                                                                <  1:591802/138‑1 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGAt                                                                                                              <  1:747491/140‑1 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGAt                                                                                                              >  2:1112070/1‑140 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGAt                                                                                                              >  1:1247986/1‑140 (MQ=255)

                        tGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGAt                                                                                                              >  1:601149/1‑140 (MQ=255)

                         gcgcTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATg                                                                                                             <  1:524820/140‑1 (MQ=255)

                         gcgcTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATg                                                                                                             <  1:744974/140‑1 (MQ=255)

                             tACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTaa                                                                                                         >  2:1474254/1‑140 (MQ=255)

                              aCTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTaaa                                                                                                        <  1:1112070/140‑1 (MQ=255)

                                  gTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAg                                                                                                    <  2:601149/140‑1 (MQ=255)

                                    tAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGt                                                                                                  >  1:903677/1‑140 (MQ=255)

                                       ttGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGAt                                                                                               >  2:281320/1‑140 (MQ=255)

                                         gTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGAGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATaa                                                                                             >  2:171544/1‑140 (MQ=255)

                                         gTTATTACTTTTTTTATGGGTTTCAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATaa                                                                                             >  1:1423920/1‑140 (MQ=255)

                                             ttaCTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATa                                                                                              <  2:1127902/135‑1 (MQ=255)

                                             ttaCTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATa                                                                                              >  1:1127902/1‑135 (MQ=255)

                                             ttaCTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATc                                                                                         >  1:1002012/1‑140 (MQ=255)

                                                  ttttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCa                                                                                    <  1:177392/140‑1 (MQ=255)

                                                  ttttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCa                                                                                    <  2:569949/140‑1 (MQ=255)

                                                    ttttATTGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCaaa                                                                                  <  1:1278235/140‑1 (MQ=255)

                                                    ttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCaa                                                                                   >  1:140847/1‑140 (MQ=255)

                                                    ttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATaaaa                                                                                           <  1:504311/131‑1 (MQ=255)

                                                    ttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATaaaa                                                                                           >  2:504311/1‑131 (MQ=255)

                                                    ttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCaaa                                                                                  >  1:500286/1‑140 (MQ=255)

                                                     tttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAg                                                                                 >  1:230604/1‑140 (MQ=255)

                                                        aTGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCa                                                                               <  2:140847/140‑1 (MQ=255)

                                                          gggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTg                                                                            >  2:1445137/1‑140 (MQ=255)

                                                           ggTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGa                                                                           >  1:789582/1‑140 (MQ=255)

                                                            gTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGaa                                                                          <  2:412292/140‑1 (MQ=255)

                                                                        tACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAAt                                                              <  2:1423164/140‑1 (MQ=255)

                                                                             gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGtttt                                                         >  1:824050/1‑140 (MQ=255)

                                                                             gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGtttt                                                         >  1:1282359/1‑140 (MQ=255)

                                                                             gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGtttt                                                         >  1:1315272/1‑140 (MQ=255)

                                                                                tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTg                                                      >  1:758029/1‑140 (MQ=255)

                                                                                   gCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaa                                                   >  2:879744/1‑140 (MQ=255)

                                                                                    cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaat                                                  <  2:1223357/140‑1 (MQ=255)

                                                                                    cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGATGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAAGCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaat                                                  <  1:1156551/140‑1 (MQ=255)

                                                                                      aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaataa                                                >  2:28319/1‑140 (MQ=255)

                                                                                       tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTaataat                                               >  2:1323907/1‑140 (MQ=255)

                                                                                        ggACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATg                                              <  1:1445137/140‑1 (MQ=255)

                                                                                        ggACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATg                                              >  1:226575/1‑140 (MQ=255)

                                                                                         gACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGc                                             <  2:1002012/140‑1 (MQ=255)

                                                                                          aCTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCa                                            <  1:281320/140‑1 (MQ=255)

                                                                                            tGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaat                                                  >  2:366435/1‑132 (MQ=255)

                                                                                            tGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGtaat                                                  <  1:366435/132‑1 (MQ=255)

                                                                                             gCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGtt                                                           <  1:1187563/122‑1 (MQ=255)

                                                                                             gCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGtt                                                           >  2:1187563/1‑122 (MQ=255)

                                                                                             gCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTa                                         >  1:1187813/1‑140 (MQ=255)

                                                                                               tATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTAcc                                       <  2:903677/140‑1 (MQ=255)

                                                                                                aTGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCa                                      <  2:1282359/140‑1 (MQ=255)

                                                                                                 tGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTAcc                                       <  2:704562/138‑1 (MQ=255)

                                                                                                 tGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAg                                     >  1:546009/1‑140 (MQ=255)

                                                                                                 tGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAg                                     >  2:1164678/1‑140 (MQ=255)

                                                                                                    tGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGcc                                  <  2:1187813/140‑1 (MQ=255)

                                                                                                     gAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 <  1:1323907/140‑1 (MQ=255)

                                                                                                     gAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 <  1:1455498/140‑1 (MQ=255)

                                                                                                     gAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 <  1:150141/140‑1 (MQ=255)

                                                                                                     gAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 <  2:915004/140‑1 (MQ=255)

                                                                                                      aaCATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTATCCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 >  1:970869/1‑140 (MQ=255)

                                                                                                      aaCATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCa                                >  2:59367/1‑140 (MQ=255)

                                                                                                      aaCATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCa                                >  2:490477/1‑140 (MQ=255)

                                                                                                      aaCATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCa                                <  2:824050/140‑1 (MQ=255)

                                                                                                        cATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtc                              >  1:1049498/1‑140 (MQ=255)

                                                                                                        cATGAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtc                              >  2:401489/1‑140 (MQ=255)

                                                                                                           gAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTATCCCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtctc                            <  2:970869/140‑1 (MQ=255)

                                                                                                           gAAGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTcc                           >  1:1143039/1‑140 (MQ=255)

                                                                                                             aGGTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAg                         <  2:71952/140‑1 (MQ=255)

                                                                                                             aGGTGGAAATACCGTTGTCATT‑GGTTACGTACCACGGCTCTCTAT‑CCCCAGCGATGGTAAAAAAGATGGTAAAATCTGGCAACACAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCACGCCCATCTCCAg                         >  2:608102/1‑140 (MQ=255)

                                                                                                              ggTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGccc                                 >  1:976978/1‑131 (MQ=255)

                                                                                                               gTGGAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGga                       <  1:1243077/140‑1 (MQ=255)

                                                                                                                  gAAATACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGagaa                    >  2:1296773/1‑140 (MQ=255)

                                                                                                                    aaTACCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGagaaga                  <  1:748277/140‑1 (MQ=255)

                                                                                                                       aCCGTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATa               <  1:490477/140‑1 (MQ=255)

                                                                                                                          gTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtctc                            <  1:1216987/124‑1 (MQ=255)

                                                                                                                          gTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCAtctc                            >  2:1216987/1‑124 (MQ=255)

                                                                                                                          gTTGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATAccc            >  1:113518/1‑140 (MQ=255)

                                                                                                                            tGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCAt          <  2:579176/140‑1 (MQ=255)

                                                                                                                            tGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCAt          <  1:1296773/140‑1 (MQ=255)

                                                                                                                            tGTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGCGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCAt          <  2:95766/140‑1 (MQ=255)

                                                                                                                             gTCATT‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAACAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATc         >  1:829470/1‑140 (MQ=255)

                                                                                                                                 tt‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCa     <  1:401489/140‑1 (MQ=255)

                                                                                                                                 tt‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCa     <  1:362285/140‑1 (MQ=255)

                                                                                                                                 tt‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCa     <  2:789582/140‑1 (MQ=255)

                                                                                                                                 tt‑GGTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCa     <  2:546009/140‑1 (MQ=255)

                                                                                                                                    ggTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATg   >  1:1461227/1‑140 (MQ=255)

                                                                                                                                     gTTGCGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATgg  <  2:113518/140‑1 (MQ=255)

TGTAGCCAACCGAGATCAATAATATGCGCTACTTGTTAATTGTTATTACTTTTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATT‑GGTTACGTACCAAGAATCTCTAT‑CCCCAGCGATGGTAAAAAAGGTGATAAAATCTGGCAAAGCAGTGAATATTTTATGAATGTTTTCTGTAATAATGCACTACCAGGCCCATCTCCAGGAGAAGAATACCCATCTGCATGG  >  NC_000913/3190609‑3190880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A