Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 224 87.5 2960670 99.3% 2939945 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,300,238 T→C S91S (AGT→AGC)  yhbQ → DNA damage response nuclease YhbQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,300,2380TC98.2% 186.6 / ‑3.5 55S91S (AGT→AGC) yhbQDNA damage response nuclease YhbQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (27/27);  minor base G (0/1);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTG  >  NC_000913/3300101‑3300377
                                                                                                                                         |                                                                                                                                           
cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCt                                                                                                                                           >  2:234391/1‑140 (MQ=255)
cTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCt                                                                                                                                           >  1:451029/1‑140 (MQ=255)
     gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa                                                                                                                                        >  2:1394750/1‑138 (MQ=255)
     gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa                                                                                                                                        <  1:466451/138‑1 (MQ=255)
     gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa                                                                                                                                        >  2:466451/1‑138 (MQ=255)
     gCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCa                                                                                                                                        <  1:1394750/138‑1 (MQ=255)
        ggTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAAccc                                                                                                                                   >  2:49870/1‑140 (MQ=255)
        ggTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAAccc                                                                                                                                   <  2:451029/140‑1 (MQ=255)
                   cgcCAGTGGGCGATCGCTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCgg                                                                                                                                <  2:240020/132‑1 (MQ=255)
                   cgcCAGTGGGCGATCGCTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCgg                                                                                                                                >  1:240020/1‑132 (MQ=255)
                      cAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa                                                                                                                        <  2:1367980/137‑1 (MQ=255)
                      cAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa                                                                                                                        >  1:1367980/1‑137 (MQ=255)
                        gTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGa                                                                                                                   <  2:951910/140‑1 (MQ=255)
                                tCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATg                                                                                                           >  2:369719/1‑140 (MQ=255)
                                tCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGCATGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATg                                                                                                           >  2:1026473/1‑140 (MQ=255)
                                   ttCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa                                                                                                                        <  1:783464/124‑1 (MQ=255)
                                   ttCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGGTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaa                                                                                                                        >  2:783464/1‑124 (MQ=255)
                                     cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaaa                                                                                                                       <  1:354467/123‑1 (MQ=255)
                                     cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTaaaa                                                                                                                       >  2:354467/1‑123 (MQ=255)
                                     cGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGt                                                                                                      <  1:1084110/140‑1 (MQ=255)
                                      gCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTg                                                                                                     >  2:359560/1‑140 (MQ=255)
                                          gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc                                                                                                 >  1:1444514/1‑140 (MQ=255)
                                          gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc                                                                                                 >  1:812383/1‑140 (MQ=255)
                                          gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc                                                                                                 >  2:862304/1‑140 (MQ=255)
                                          gCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATAc                                                                                                 <  1:1026473/140‑1 (MQ=255)
                                               aCGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAAc                                                                                            <  1:1290920/140‑1 (MQ=255)
                                                  ggCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTAGTGATACTCAACCAg                                                                                         <  1:49870/140‑1 (MQ=255)
                                                   gCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAgg                                                                                        <  2:812383/140‑1 (MQ=255)
                                                      gAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCg                                                                                     >  2:1178909/1‑140 (MQ=255)
                                                       aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGGCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg                                                                                    <  2:1229106/140‑1 (MQ=255)
                                                       aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg                                                                                    <  2:1444514/140‑1 (MQ=255)
                                                       aaTATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCgg                                                                                    <  1:1026372/140‑1 (MQ=255)
                                                          atCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTaa                                                                                 <  1:359560/140‑1 (MQ=255)
                                                           tCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAAc                                                                                <  1:234391/140‑1 (MQ=255)
                                                            cgcgTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACg                                                                               <  1:1178909/140‑1 (MQ=255)
                                                                 tAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCAtt                                                                          >  2:830676/1‑140 (MQ=255)
                                                                   aGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCa                                                                        >  1:462875/1‑140 (MQ=255)
                                                                         tGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGt                                                                  >  1:1259113/1‑140 (MQ=255)
                                                                          gACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTc                                                                 <  1:1123782/140‑1 (MQ=255)
                                                                                aCGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGc                                                           >  1:932491/1‑140 (MQ=255)
                                                                                 cGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCt                                                          <  1:601730/140‑1 (MQ=255)
                                                                                      aaaaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACg                                                     <  2:315016/140‑1 (MQ=255)
                                                                                        aaGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGAt                                                   <  2:149502/140‑1 (MQ=255)
                                                                                                cTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTgg                                           <  2:118904/140‑1 (MQ=255)
                                                                                                      gCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGgcg                                     <  2:932491/140‑1 (MQ=255)
                                                                                                            ggCGCAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCa                               >  1:1012457/1‑140 (MQ=255)
                                                                                                              cgcAGGGTTTGCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGt                             >  2:124728/1‑140 (MQ=255)
                                                                                                                        gCAGAGCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAg                   <  2:462875/140‑1 (MQ=255)
                                                                                                                             gCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCa              >  1:1241714/1‑140 (MQ=255)
                                                                                                                             gCTGTTAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCa              >  1:1041369/1‑140 (MQ=255)
                                                                                                                                 ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGGAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc          >  1:973020/1‑140 (MQ=255)
                                                                                                                                 ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc          <  1:369719/140‑1 (MQ=255)
                                                                                                                                 ttAAGCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGc          >  1:652927/1‑140 (MQ=255)
                                                                                                                                    agcagcCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGa       <  2:1012457/140‑1 (MQ=255)
                                                                                                                                     gCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGcc                     <  2:810373/125‑1 (MQ=255)
                                                                                                                                     gCAGCCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGcc                     >  1:810373/1‑125 (MQ=255)
                                                                                                                                         tCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTTCCCGGCCAGCGGCAACGCAGATCGTg  >  2:378820/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATCGTTCGCTGGCGTTACGGGCGGAATATCGCGTTAAGCAACTGACCAAACGGCAAAAAGAGCGTCTGGTAGCGGAAGGCGCAGGGTTTGCAGAGCTGTTAAGCAGTCTGCAAACCCCGGAGATTAAAAGCGATTGAAATGCTCGTGATACTCAACCAGGCCGGTAACGCCATTCAGCGCGTCATCCGCTAAACGATGTACCTGGAAGGCGCTTTCAGTGCCCGGCCAGCGGCAACGCAGATCGTG  >  NC_000913/3300101‑3300377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A