Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F28 I1 R1 224 87.5 2960670 99.3% 2939945 138.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,422,914 A→G E23E (GAA→GAG)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,422,9140AG100.0% 160.3 / NA 47E23E (GAA→GAG) ulaEL‑ribulose‑5‑phosphate 3‑epimerase UlaE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/14);  total (33/14)

TTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGG  >  NC_000913/4422779‑4423048
                                                                                                                                       |                                                                                                                                      
ttCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGcc                                                                                                                                    <  2:1326947/140‑1 (MQ=255)
                  aCGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGt                                                                                                                  >  1:1448952/1‑140 (MQ=255)
                                   ccATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTc                                                                                                 >  2:341690/1‑140 (MQ=255)
                                        gCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAAt                                                                                            >  1:1041719/1‑140 (MQ=255)
                                            aaCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCg                                                                                        >  2:351638/1‑140 (MQ=255)
                                            aaCTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCg                                                                                        >  1:491972/1‑140 (MQ=255)
                                              cTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGt                                                                                      >  2:860763/1‑140 (MQ=255)
                                                 tgGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGa                                                                                   >  2:27551/1‑140 (MQ=255)
                                                  ggggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGAt                                                                                  >  1:869507/1‑140 (MQ=255)
                                                   gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  2:95512/1‑140 (MQ=255)
                                                   gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  1:526655/1‑140 (MQ=255)
                                                   gggCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATg                                                                                 >  1:241047/1‑140 (MQ=255)
                                                      cTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              <  2:241047/140‑1 (MQ=255)
                                                       tAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAAc                                                                             <  2:869507/140‑1 (MQ=255)
                                                        aaGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACt                                                                            >  1:264049/1‑140 (MQ=255)
                                                           gAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGAc                                                                         >  1:692477/1‑140 (MQ=255)
                                                              cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              >  1:1260344/1‑132 (MQ=255)
                                                              cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGaaa                                                                              <  2:1260344/132‑1 (MQ=255)
                                                              cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa                                                                          <  2:1213964/136‑1 (MQ=255)
                                                              cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGa                                                                          >  1:1213964/1‑136 (MQ=255)
                                                              cGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGAt                                                                      >  1:342466/1‑140 (MQ=255)
                                                               ggATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATc                                                                     >  2:504509/1‑140 (MQ=255)
                                                                     gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGt                                                               <  1:95512/140‑1 (MQ=255)
                                                                     gttgtCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGt                                                               >  2:650814/1‑140 (MQ=255)
                                                                         tCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTcgcg                                                           >  2:1005470/1‑140 (MQ=255)
                                                                          ccAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCgcgc                                                          >  1:1171085/1‑140 (MQ=255)
                                                                              acaaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCa                                                      >  2:1055431/1‑140 (MQ=255)
                                                                              acaaaTCCCGCTTGGCATC‑‑TGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAAc                                                    >  1:1170170/1‑140 (MQ=255)
                                                                                aaaTCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAAc                                                    >  2:512601/1‑140 (MQ=255)
                                                                                   tCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTgg                                                 >  1:824957/1‑140 (MQ=255)
                                                                                           gccATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGa                                         <  2:1001350/138‑1 (MQ=255)
                                                                                            gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                        >  1:80320/1‑140 (MQ=255)
                                                                                            gCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGag                                        >  1:301136/1‑140 (MQ=255)
                                                                                                   tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  1:1055431/140‑1 (MQ=255)
                                                                                                   tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  2:93332/140‑1 (MQ=255)
                                                                                                   tGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTc                                 <  2:301136/140‑1 (MQ=255)
                                                                                                       aaaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgc                             <  2:1171085/140‑1 (MQ=255)
                                                                                                        aaGCGCTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGgcg                            <  1:27551/140‑1 (MQ=255)
                                                                                                          gcgcTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCt                          >  2:71726/1‑140 (MQ=255)
                                                                                                           cgcTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTg                         <  1:341690/140‑1 (MQ=255)
                                                                                                             cTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                       >  1:702803/1‑140 (MQ=255)
                                                                                                             cTCCCCGCCGGGGAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGt                       >  2:951356/1‑140 (MQ=255)
                                                                                                                        ggAGTGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGAttg            >  2:1278746/1‑140 (MQ=255)
                                                                                                                            tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  1:1209698/1‑140 (MQ=255)
                                                                                                                            tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  1:1077556/1‑140 (MQ=255)
                                                                                                                            tGCTGGCTGGAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGa        >  2:1002939/1‑140 (MQ=255)
                                                                                                                              ctggctggAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGaaa      <  1:1005470/140‑1 (MQ=255)
                                                                                                                                  ctggAGCGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCgg  >  1:1218400/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGCAAAAACGTTAGGCTTCGATTTTGTCGAAATGTCGGTAGATGAAACTGACGATCGCCTGTCGCGCCTCAACTGGAGCCGCGAGCAGCGTCTGGCGCTGGTCAATGCGATTGTTGAAACCGG  >  NC_000913/4422779‑4423048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A