Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	138	91.1	3071990	99.2%	3047414	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,650,599	(A)_5→4	coding (363/426 nt)	arsC →	arsenate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,650,595	0	A	.	98.6%	294.8 / ‑2.4	73	coding (359/426 nt)	arsC	arsenate reductase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base . (37/35); total (37/36)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCG  >  NC_000913/3650457‑3650730

aTAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCaa                                                                                                                                         <  2:476184/140‑1 (MQ=255)

      ttACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCaaaaggcg                                                                                                                                   <  2:743082/140‑5 (MQ=255)

       tACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGgcgc                                                                                                                                 >  2:1271057/1‑140 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:623642/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:325456/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:279584/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:367678/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  2:8850/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1447558/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1271057/140‑1 (MQ=255)

        aCTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCa                                                                                                                                <  1:1125207/140‑1 (MQ=255)

        aCTGACGATCGATTAATAGAGTTTACGCTTGAGCAGCCGATTCTGATTAATCGCGTGATTGTGGTGACGTGGTTGGGAACTCGGCTGTGCCGCCCTTCAGAAGTGGTGCTCGAAATTCTGCCAGATGCG‑AAAAAGGCGCa                                                                                                                                <  2:939453/140‑1 (MQ=255)

         cTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAt                                                                                                                               >  2:445901/1‑140 (MQ=255)

         cTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCAATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAt                                                                                                                               >  1:196593/1‑140 (MQ=255)

          tGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCAtt                                                                                                                              <  1:1273669/140‑1 (MQ=255)

              gATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTcc                                                                                                                          >  2:903540/1‑140 (MQ=255)

              gATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTcc                                                                                                                          >  2:115713/1‑140 (MQ=255)

               aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  2:597670/1‑140 (MQ=255)

               aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  2:589468/1‑140 (MQ=255)

               aTCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCa                                                                                                                         >  1:1298273/1‑140 (MQ=255)

                  ggTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAgg                                                                                                                      <  2:409133/140‑1 (MQ=255)

                    ttAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    >  1:385815/1‑140 (MQ=255)

                      aaTCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGa                                                                                                                  <  1:907696/140‑1 (MQ=255)

                        tCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATg                                                                                                                >  1:1314454/1‑140 (MQ=255)

                          gACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGc                                                                                                              >  1:911705/1‑140 (MQ=255)

                            cTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCga                                                                                                            >  2:33800/1‑140 (MQ=255)

                             tttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgag                                                                                                           >  2:456722/1‑140 (MQ=255)

                              ttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgaga                                                                                                          >  2:328453/1‑140 (MQ=255)

                              ttATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCgaga                                                                                                          >  2:1022879/1‑140 (MQ=255)

                                aTGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGaaa                                                                                                        >  2:864529/1‑140 (MQ=255)

                                aTGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGaaa                                                                                                        <  1:328453/140‑1 (MQ=255)

                                    ttCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    <  1:718924/124‑1 (MQ=255)

                                    ttCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGaa                                                                                                                    >  2:718924/1‑124 (MQ=255)

                                     tCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGt                                                                                                   <  2:187357/140‑1 (MQ=255)

                                      cAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGtt                                                                                                  >  1:706492/1‑140 (MQ=255)

                                        gCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTtga                                                                                                <  1:153842/140‑1 (MQ=255)

                                         cACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTtgat                                                                                               >  1:644571/1‑140 (MQ=255)

                                            ccGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGaa                                                                                            <  2:911705/140‑1 (MQ=255)

                                              gATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGc                                                                                          >  2:405249/1‑140 (MQ=255)

                                              gATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGc                                                                                          >  1:1189957/1‑140 (MQ=255)

                                                    gATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTaa                                                                                    <  2:505021/140‑1 (MQ=255)

                                                     aTTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAg                                                                                   <  1:903540/140‑1 (MQ=255)

                                                     aTTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAg                                                                                   <  1:115713/140‑1 (MQ=255)

                                                         aTCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGcc                                                                               >  1:923411/1‑140 (MQ=255)

                                                          tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCt                                                                              >  1:1046434/1‑140 (MQ=255)

                                                          tCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCt                                                                              >  2:1467090/1‑140 (MQ=255)

                                                             cccGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGaa                                                                           >  1:758280/1‑140 (MQ=255)

                                                                     tggtgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcg                                                                   >  2:987016/1‑140 (MQ=255)

                                                                      ggtgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcgg                                                                  <  1:589468/140‑1 (MQ=255)

                                                                        tgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcggcg                                                                <  1:405249/140‑1 (MQ=255)

                                                                        tgACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAgcggcg                                                                <  2:385815/140‑1 (MQ=255)

                                                                          aCGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              <  1:33800/140‑1 (MQ=255)

                                                                           cgccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATa                                                                        <  2:1479152/129‑1 (MQ=255)

                                                                           cgccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATa                                                                        >  1:1479152/1‑129 (MQ=255)

                                                                            gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGatat                                                            >  2:1405539/1‑140 (MQ=255)

                                                                            gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              <  1:629092/138‑1 (MQ=255)

                                                                            gccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGat                                                              >  2:629092/1‑138 (MQ=255)

                                                                             ccgcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATc                                                           <  2:276384/140‑1 (MQ=255)

                                                                               gcTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATccc                                                         <  2:1298273/140‑1 (MQ=255)

                                                                                    gAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCca                                                    <  1:1351488/140‑1 (MQ=255)

                                                                                        tCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAgg                                                >  1:716008/1‑140 (MQ=255)

                                                                                         cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  1:1022879/140‑1 (MQ=255)

                                                                                         cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  2:1227968/140‑1 (MQ=255)

                                                                                         cGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgt                                               <  2:706492/140‑1 (MQ=255)

                                                                                             tgtgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTATCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGgttgtt                                           >  2:294734/1‑140 (MQ=255)

                                                                                               tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAg                                         >  1:757824/1‑140 (MQ=255)

                                                                                               tgCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAg                                         >  2:401278/1‑140 (MQ=255)

                                                                                                    cccTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAg                                   >  2:848031/1‑140 (MQ=255)

                                                                                                     ccTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGt                                  <  2:644571/140‑1 (MQ=255)

                                                                                                         cAGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTgcgc                              <  2:1046434/140‑1 (MQ=255)

                                                                                                          aGAAGTGGTGCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCt                             <  1:445901/140‑1 (MQ=255)

                                                                                                                 gtgCTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAGAAAAAGTGCGCtttattt                       >  1:1507157/1‑140 (MQ=255)

                                                                                                                    cTGGAAATTCTGCCAGATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGt                   >  2:1366564/1‑140 (MQ=255)

                                                                                                                                   gATGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATAT‑CCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGgc   <  1:118244/140‑1 (MQ=255)

                                                                                                                                     tGCGC‑AAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGgcg  >  2:1213957/1‑140 (MQ=255)

ATAAATTTACTGACGATCGGTTAATCGACTTTATGCTTCAGCACCCGATTCTGATTAATCGCCCGATTGTGGTGACGCCGCTGGGAACTCGCCTGTGCCGCCCTTCAGAAGTGGTGCTGGAAATTCTGCCAGATGCGCAAAAAGGCGCATTCTCCAAGGAAGATGGCGAGAAAGTGGTTGATGAAGCGGGTAAGCGCCTGAAATAAAGCGGCGATATCCCCCCCACAGGTTGTTAG‑AAAAGTGCGCTTTATTTATGTCGGATGCGACGCTGGCG  >  NC_000913/3650457‑3650730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A