Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,840,934	T→C	Y471H (TAT→CAT)	ascF →	beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,840,934	0	T	C	100.0%	169.3 / NA	49	Y471H (TAT→CAT)	ascF	beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/21); total (28/21)

CGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGA  >  NC_000913/2840798‑2841069

cGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATc                                                                                                                                      <  1:181077/140‑1 (MQ=255)

 gATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCa                                                                                                                                     <  2:534405/140‑1 (MQ=255)

   tCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCaga                                                                                                                                   <  2:1096882/140‑1 (MQ=255)

     cGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGc                                                                                                                                 >  2:354821/1‑140 (MQ=255)

       gCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGt                                                                                                                               >  1:8254/1‑140 (MQ=255)

            tCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAac                                                                                                                          >  1:1024852/1‑140 (MQ=255)

            tCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAac                                                                                                                          <  1:540252/140‑1 (MQ=255)

                 tGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAaccgacc                                                                                                                     >  1:355896/1‑140 (MQ=255)

                       tCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGcc                                                                                                               >  2:373648/1‑140 (MQ=255)

                         gTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             >  2:330485/1‑140 (MQ=255)

                          tCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             <  1:225884/139‑1 (MQ=255)

                          tCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCaa                                                                                                             >  2:225884/1‑139 (MQ=255)

                            tGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAaaga                                                                                                          >  1:994048/1‑140 (MQ=255)

                              ggTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAaagaag                                                                                                        <  2:994048/140‑1 (MQ=255)

                                tgtTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGaagta                                                                                                      <  1:330485/140‑1 (MQ=255)

                                   tCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGaagtaagt                                                                                                   <  1:810100/140‑1 (MQ=255)

                                    cgcgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTc                                                                                                  <  2:8254/140‑1 (MQ=255)

                                    cgcgGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTc                                                                                                  >  1:370881/1‑140 (MQ=255)

                                        gTCATGGCGCTGGCGGGGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGGTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCCAAGAAGTAAGTCTTaa                                                                                              >  2:415455/1‑140 (MQ=255)

                                              gcgcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAgg                                                                                        <  2:1024852/140‑1 (MQ=255)

                                                 cTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATg                                                                                     >  2:578411/1‑140 (MQ=255)

                                                         ggtgCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTc                                                                             <  2:355896/140‑1 (MQ=255)

                                                                tCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTAttt                                                                      >  2:518413/1‑140 (MQ=255)

                                                                     tATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGa                                                                 <  1:713770/140‑1 (MQ=255)

                                                                          tCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGtt                                                            >  2:156745/1‑140 (MQ=255)

                                                                              acTGTTGCTCGGCTTTCAGGATATTCCTGTTGAGGAAGCGGCTGCACCGGCGCGAACGCATCGGAGCGTACAACCGACCGTCGCCAAAGGAGCGAGTCTTAATTGAGGATGAAAATGTCAGTCTTTGGAGACAGtttttt                                                        >  2:520594/1‑140 (MQ=255)

                                                                                  ttGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATgg                                                    >  1:1026271/1‑140 (MQ=255)

                                                                                  ttGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATgg                                                    >  1:325548/1‑140 (MQ=255)

                                                                                   tGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGCAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGAATTTCCAGAAAGTTTTTTATggg                                                   >  2:757800/1‑140 (MQ=255)

                                                                                   tGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATggg                                                   >  2:864484/1‑140 (MQ=255)

                                                                                     cTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGggc                                                 >  2:546025/1‑140 (MQ=255)

                                                                                     cTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGggc                                                 >  1:900436/1‑140 (MQ=255)

                                                                                          cTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGgcgc                                            >  1:947188/1‑140 (MQ=255)

                                                                                               aGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTg                                       <  1:546025/140‑1 (MQ=255)

                                                                                               aGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTg                                       <  1:848854/140‑1 (MQ=255)

                                                                                                    atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  >  2:542533/1‑140 (MQ=255)

                                                                                                    atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  <  2:947188/140‑1 (MQ=255)

                                                                                                    atTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTgccgcc                                  >  2:461765/1‑140 (MQ=255)

                                                                                                       ccTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               <  1:646952/140‑1 (MQ=255)

                                                                                                        cTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAcc                              >  2:929860/1‑140 (MQ=255)

                                                                                                           ttGAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGt                           >  2:539462/1‑140 (MQ=255)

                                                                                                             gAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               >  2:123946/1‑134 (MQ=255)

                                                                                                             gAGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAAc                               <  1:123946/134‑1 (MQ=255)

                                                                                                              aGGAAGCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTg                        >  1:787422/1‑140 (MQ=255)

                                                                                                                   gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   >  1:588882/1‑140 (MQ=255)

                                                                                                                   gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   <  1:373648/140‑1 (MQ=255)

                                                                                                                   gCGGCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGt                   <  1:415455/140‑1 (MQ=255)

                                                                                                                     ggCTGCCCAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGc                 >  2:22968/1‑140 (MQ=255)

                                                                                                                            cAGGCGCGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGt          <  2:370881/140‑1 (MQ=255)

                                                                                                                               gcgcGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGaa       >  1:551419/1‑140 (MQ=255)

                                                                                                                               gcgcGAAAGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGaa       <  1:864484/140‑1 (MQ=255)

                                                                                                                                    aaaGCATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGa  >  2:472086/1‑140 (MQ=255)

CGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGA  >  NC_000913/2840798‑2841069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A