Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,303,947	T→C	V167A (GTG→GCG)	yhbW →	putative luciferase‑like monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,303,947	0	T	C	100.0%	152.6 / NA	45	V167A (GTG→GCG)	yhbW	putative luciferase‑like monooxygenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/22); total (23/22)

TGGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGTGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAACC  >  NC_000913/3303812‑3304079

tgGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTgg                                                                                                                                  <  1:1143442/140‑1 (MQ=255)

       aCGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCgcgt                                                                                                                                    >  1:832526/1‑131 (MQ=255)

        cgtcgtCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAgg                                                                                                                          >  1:995462/1‑140 (MQ=255)

        cgtcgtCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAgg                                                                                                                          >  1:546291/1‑140 (MQ=255)

          tcgtcATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  2:488357/140‑1 (MQ=255)

          tcgtcATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  1:795608/140‑1 (MQ=255)

                      cggcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACa                                                                                                            >  1:64825/1‑140 (MQ=255)

                        gcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgc                                                                                                          >  1:1144248/1‑140 (MQ=255)

                        gcgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgc                                                                                                          >  2:925777/1‑140 (MQ=255)

                         cgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        <  1:1065774/125‑1 (MQ=255)

                         cgATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCt                                                                                                                        >  2:1065774/1‑125 (MQ=255)

                           atatTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAgcgcg                                                                                                       >  1:970851/1‑140 (MQ=255)

                                 aTAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTg                                                                                                 <  2:1144248/140‑1 (MQ=255)

                                          cccGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAg                                                                                        >  2:622984/1‑140 (MQ=255)

                                           ccGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       <  2:546291/140‑1 (MQ=255)

                                               gATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCC‑‑‑CCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcg                                                                                              <  1:69109/126‑1 (MQ=255)

                                               gATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCC‑‑‑CCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcg                                                                                              >  2:69109/1‑126 (MQ=255)

                                                        gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcggc                                                                                            <  1:1131369/122‑1 (MQ=255)

                                                        gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTggcggc                                                                                            >  2:1131369/1‑122 (MQ=255)

                                                        gAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtt                                                                          <  1:622984/140‑1 (MQ=255)

                                                         aGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgttt                                                                         >  2:897575/1‑140 (MQ=255)

                                                           cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGGCCGGTACCAGGCTATGGCGCGAAAAACCCCGCGTGGTTGTTAGGCCCCAGCCCTTTCAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgttttc                                                                       >  2:322363/1‑138 (MQ=255)

                                                           cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       <  1:763012/124‑1 (MQ=255)

                                                           cTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGc                                                                                       >  2:763012/1‑124 (MQ=255)

                                                               tggACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   >  1:851185/1‑140 (MQ=255)

                                                                     ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             <  1:897575/140‑1 (MQ=255)

                                                                     ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             >  1:351067/1‑140 (MQ=255)

                                                                     ggTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTca                                                             <  2:970851/140‑1 (MQ=255)

                                                                        ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   >  1:896489/1‑131 (MQ=255)

                                                                        ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCcgtttgcgttt                                                                   <  2:896489/131‑1 (MQ=255)

                                                                        ttGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCacac                                                          >  2:727681/1‑140 (MQ=255)

                                                                               ccGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCgcgc                                                   <  2:64825/140‑1 (MQ=255)

                                                                                         aaTCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGa                                               >  2:807833/1‑134 (MQ=255)

                                                                                         aaTCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGa                                               <  1:807833/134‑1 (MQ=255)

                                                                                               cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   >  2:857446/1‑140 (MQ=255)

                                                                                               cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   <  2:995462/140‑1 (MQ=255)

                                                                                               cATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCa                                   >  2:105609/1‑140 (MQ=255)

                                                                                                 tgtgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAgg                                 <  1:274010/140‑1 (MQ=255)

                                                                                                  gtgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGgc                                <  2:851185/140‑1 (MQ=255)

                                                                                                   tgCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGgcg                               <  1:322363/140‑1 (MQ=255)

                                                                                                      gcCCGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTg                            <  1:704942/140‑1 (MQ=255)

                                                                                                       cccGGTACCAGGCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGc                           <  1:105609/140‑1 (MQ=255)

                                                                                                                  gCTATGGCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCgc                >  2:708885/1‑140 (MQ=255)

                                                                                                                      tGGCGAGAAAATCCCCGCGTGGTTGTTAGGTTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCgcagca            >  2:49799/1‑140 (MQ=255)

                                                                                                                       ggCGAGAAAATCCCCGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCaa           >  2:731923/1‑140 (MQ=255)

                                                                                                                                  ccccGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAAcc  <  2:328321/138‑1 (MQ=255)

                                                                                                                                  ccccGCGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAAcc  >  1:328321/1‑138 (MQ=255)

TGGCGCTACGTCGTCATATGAGCGGCGATATTGATAATTTCCCCCGCGATGTGGCGGAGCTGGTGGACTGGTTTGACGCCCGCGATCCCAATCCGCATGTGCGCCCGGTACCAGGCTATGGCGAGAAAATCCCCGTGTGGTTGTTAGGCTCCAGCCTTTACAGCGCGCAACTGGCGGCGCAGCTTGGTCTGCCGTTTGCGTTTGCCTCACACTTCGCGCCGGATATGCTGTTCCAGGCGCTGCATCTTTATCGCAGCAACTTCAAACC  >  NC_000913/3303812‑3304079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A