Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 172 67.8 2301510 98.9% 2276193 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,555,953 T→C E299E (GAA→GAG)  rtcA ← RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,555,9530TC98.3% 186.4 / ‑4.3 59E299E (GAA→GAG) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (30/28);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

ACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG  >  NC_000913/3555822‑3556084
                                                                                                                                   |                                                                                                                                   
aCATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCcgc                                                                                                                             <  2:247614/140‑1 (MQ=255)
 cATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCcgcg                                                                                                                            <  1:822708/140‑1 (MQ=255)
      ctGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc                                                                                                                       >  1:901037/1‑140 (MQ=255)
          atcatTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAgc                                                                                                                   <  1:1011817/140‑1 (MQ=255)
              ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTccc                                                                                                                                 >  1:988148/1‑122 (MQ=255)
              ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTccc                                                                                                                                 <  2:988148/122‑1 (MQ=255)
              ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCa                                                                                                               >  2:768639/1‑140 (MQ=255)
              ttCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCa                                                                                                               >  1:590517/1‑140 (MQ=255)
               tCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGACATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCAt                                                                                                              >  1:866047/1‑140 (MQ=255)
                 aaTGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCg                                                                                                            >  1:1150740/1‑140 (MQ=255)
                      tcccccGCGTTACGCCATCTGTTTCTACCAAACTAAACCGCACCGGCAAGACACGCTCCCCCACCTCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGc                                                                                                       <  2:577497/137‑1 (MQ=255)
                      tCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGc                                                                                                       >  2:902096/1‑140 (MQ=255)
                       cACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCa                                                                                                      >  1:280794/1‑140 (MQ=255)
                        aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc                                                                                                                       <  2:687414/122‑1 (MQ=255)
                        aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGcgcccgc                                                                                                                       >  1:687414/1‑122 (MQ=255)
                        aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAc                                                                                                     >  1:880017/1‑140 (MQ=255)
                        aCCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCAc                                                                                                     >  2:469427/1‑140 (MQ=255)
                             cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCg                                                                                                            <  2:928370/128‑1 (MQ=255)
                             cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGtt                                                                                                >  2:441005/1‑140 (MQ=255)
                             cgTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCACCAGCGCCATCg                                                                                                            >  1:928370/1‑128 (MQ=255)
                              gTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTg                                                                                               <  1:238316/140‑1 (MQ=255)
                                tACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                             <  1:441005/140‑1 (MQ=255)
                                tACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGt                                                                                             <  2:901037/140‑1 (MQ=255)
                                     cATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCa                                                                                        <  2:280794/140‑1 (MQ=255)
                                        cTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGa                                                                                     <  1:311070/140‑1 (MQ=255)
                                         tGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGat                                                                                    <  2:107332/140‑1 (MQ=255)
                                          gTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata                                                                                   >  1:981523/1‑140 (MQ=255)
                                            ttCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata                                                                                   >  1:762050/1‑138 (MQ=255)
                                            ttCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGata                                                                                   <  2:762050/138‑1 (MQ=255)
                                              cTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTcc                                                                               <  1:902096/140‑1 (MQ=255)
                                               tATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccc                                                                              >  1:290699/1‑140 (MQ=255)
                                                 tCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTccccc                                                                            >  2:233475/1‑140 (MQ=255)
                                                        aaaCCGCCCCGTCAAGAAACGCCCCACCTCCGCGATTTTGGTCAGCAGATGGCAGGGGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg                                                                     <  2:829854/140‑1 (MQ=255)
                                                              cACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTTCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg                                                                     >  1:388369/1‑134 (MQ=255)
                                                              cACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCAccgccg                                                                     <  2:388369/134‑1 (MQ=255)
                                                               aCCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCt                                                              >  1:658542/1‑140 (MQ=255)
                                                                   gCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGcc                                                          >  1:160607/1‑140 (MQ=255)
                                                                          aCGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGAGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGc                                                   <  1:907113/140‑1 (MQ=255)
                                                                              tCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGttt                                               >  1:1101357/1‑140 (MQ=255)
                                                                              tCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGttt                                               >  2:466140/1‑140 (MQ=255)
                                                                               ccaccaCCGCAATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTc                                              <  2:563699/140‑1 (MQ=255)
                                                                                 accaccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCAc                                            <  2:154842/140‑1 (MQ=255)
                                                                                   caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACct                                          <  2:229962/140‑1 (MQ=255)
                                                                                   caccGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACct                                          >  1:229962/1‑140 (MQ=255)
                                                                                      cgcgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCtt                                       >  1:1130995/1‑140 (MQ=255)
                                                                                        cgATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTc                                     >  1:292768/1‑140 (MQ=255)
                                                                                           tatTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCAcc                                  <  1:768639/140‑1 (MQ=255)
                                                                                               ggTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACt                              <  1:1058627/140‑1 (MQ=255)
                                                                                                gTCAGCAGATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACtg                             >  2:822512/1‑140 (MQ=255)
                                                                                                   agcagATGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGc                          <  2:880017/140‑1 (MQ=255)
                                                                                                        aTGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCaa                     >  2:1002202/1‑140 (MQ=255)
                                                                                                        aTGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCaa                     >  2:934290/1‑140 (MQ=255)
                                                                                                         tGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                    <  1:934290/140‑1 (MQ=255)
                                                                                                         tGGCATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                    <  1:469427/140‑1 (MQ=255)
                                                                                                         tGGCATGAGGGATGGGCGACCGTAAACCCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAac                    <  2:867932/140‑1 (MQ=255)
                                                                                                            cATGAGGGATGGGCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAaccac                 <  1:466140/140‑1 (MQ=255)
                                                                                                                     tGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACt        >  2:454471/1‑140 (MQ=255)
                                                                                                                      gggCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTg       >  1:577862/1‑140 (MQ=255)
                                                                                                                        gCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc     <  1:454471/140‑1 (MQ=255)
                                                                                                                        gCGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAc     <  2:1150740/140‑1 (MQ=255)
                                                                                                                         cGACCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg    <  1:822512/140‑1 (MQ=255)
                                                                                                                           aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcgcg  <  2:981523/140‑1 (MQ=255)
                                                                                                                           aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCA‑CAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg    >  2:252452/1‑137 (MQ=255)
                                                                                                                           aCCGTAAACTCCCCCGCGCCCGCCAGCGCCATCGGTAGCA‑CAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGAcg    <  1:252452/137‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                   
ACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAACTAAACCGCACCGGCAAGAAACGCTCCACCACCGCGATATTGGTCAGCAGATGGCATGAGGGATGGGCGACCGTAAATTCCCCCGCGCCCGCCAGCGCCATCGGTAGCACCAGTTGGTCAGCAAGATATTCCCCCACCGCCGCCGTGCTTGCCAGGTAGCGTTTCACCTCTTTCACCAACTGTGCCGCAACCACCTCGGCACTGACGCG  >  NC_000913/3555822‑3556084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A