Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,561,301	T→C	E51G (GAG→GGG)	glpG ←	rhomboid protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,561,301	0	T	C	100.0%	234.3 / NA	68	E51G (GAG→GGG)	glpG	rhomboid protease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/35); total (33/35)

CGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAA  >  NC_000913/3561168‑3561433

cGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGccc                                                                                                                                >  1:1010387/1‑140 (MQ=255)

cGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGccc                                                                                                                                >  1:1033200/1‑140 (MQ=255)

        cgcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACgcgc                                                                                                                        >  2:638649/1‑140 (MQ=255)

        cgcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACgcgc                                                                                                                        >  2:47676/1‑140 (MQ=255)

        cgcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACgcgc                                                                                                                        >  2:296222/1‑140 (MQ=255)

         gcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCt                                                                                                                       <  2:190268/140‑1 (MQ=255)

         gcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCt                                                                                                                       <  2:1102615/140‑1 (MQ=255)

         gcgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCt                                                                                                                       <  1:638649/140‑1 (MQ=255)

         ccgTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAACCGCGCCAGCCCCGCCCGTACGCGCt                                                                                                                       <  2:1108239/139‑1 (MQ=255)

                 gCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTgg                                                                                                               <  2:938248/140‑1 (MQ=255)

                       cggcAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCa                                                                                                         >  1:1133701/1‑140 (MQ=255)

                       cggcAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCa                                                                                                         >  1:1136046/1‑140 (MQ=255)

                            aagaaagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgc                                                                                                    <  1:296222/140‑1 (MQ=255)

                               aaagGATAACGGCGATAATGCAGGCCACTGCCGGAATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag                                                                                                 >  2:1018709/1‑140 (MQ=255)

                                aagGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCCGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccagc                                                                                                <  2:954220/140‑1 (MQ=255)

                                    aTAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            >  1:1092093/1‑140 (MQ=255)

                                    aTAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            <  2:1136046/140‑1 (MQ=255)

                                       aCGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                         <  2:291010/140‑1 (MQ=255)

                                       aCGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                         >  1:548287/1‑140 (MQ=255)

                                         ggCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            >  1:46639/1‑135 (MQ=255)

                                         ggCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            <  2:46639/135‑1 (MQ=255)

                                          gCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGc                                                                                      <  2:1092093/140‑1 (MQ=255)

                                             aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag                                                                                                 >  2:448517/1‑126 (MQ=255)

                                             aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCgccag                                                                                                 <  1:448517/126‑1 (MQ=255)

                                             aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            >  2:1003783/1‑131 (MQ=255)

                                             aTAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGa                                                                                            <  1:1003783/131‑1 (MQ=255)

                                                aTGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGt                                                                                <  1:1041251/140‑1 (MQ=255)

                                                 tGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt                                                                               >  1:479768/1‑140 (MQ=255)

                                                 tGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGtt                                                                               >  1:903631/1‑140 (MQ=255)

                                                  gCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTa                                                                              <  2:1010387/140‑1 (MQ=255)

                                                     ggCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                         >  1:775347/1‑126 (MQ=255)

                                                     ggCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACAt                                                                                         <  2:775347/126‑1 (MQ=255)

                                                       ccACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTAtgttg                                                                         >  1:433470/1‑140 (MQ=255)

                                                          cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                                                                      >  1:681986/1‑140 (MQ=255)

                                                          cTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGttgttg                                                                      >  2:671193/1‑140 (MQ=255)

                                                           tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAACCGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGa                                                                     <  2:425447/140‑1 (MQ=255)

                                                           tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCt                                                                                     <  2:478219/124‑1 (MQ=255)

                                                           tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCt                                                                                     >  1:478219/1‑124 (MQ=255)

                                                           tGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGC‑TTGGTTATGTTGTTGaa                                                                    >  2:968165/1‑140 (MQ=255)

                                                                  aTGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa                                                              <  1:788443/140‑1 (MQ=255)

                                                                     gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCTGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa                                                              <  1:391872/137‑1 (MQ=255)

                                                                     gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGa                                                              >  2:391872/1‑137 (MQ=255)

                                                                     gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa                                                           >  1:970777/1‑140 (MQ=255)

                                                                     gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa                                                           >  1:407346/1‑140 (MQ=255)

                                                                     gcctgcctGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGa                                                           >  2:594328/1‑140 (MQ=255)

                                                                      cctgcctgCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGAt                                                          <  2:717373/140‑1 (MQ=255)

                                                                          cctgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAaca                                                      >  2:1136387/1‑140 (MQ=255)

                                                                            tgccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAcc                                                    <  2:433470/140‑1 (MQ=255)

                                                                             gccAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACAccc                                                   >  1:949862/1‑140 (MQ=255)

                                                                               cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg                                                 <  1:1136387/140‑1 (MQ=255)

                                                                               cAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTg                                                 >  1:521400/1‑140 (MQ=255)

                                                                                 gCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCg                                               <  1:1018709/140‑1 (MQ=255)

                                                                                    cgccgcCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCg                                            <  2:949862/140‑1 (MQ=255)

                                                                                      ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGcc                                          <  1:594328/140‑1 (MQ=255)

                                                                                      ccgccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGcc                                          <  2:903631/140‑1 (MQ=255)

                                                                                        gccAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCAt                                        >  1:820496/1‑140 (MQ=255)

                                                                                                  gcgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGTGTCGCCATGTAATcaaca                              <  2:548287/140‑1 (MQ=255)

                                                                                                   cgGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATcaacaa                             >  1:461296/1‑140 (MQ=255)

                                                                                                         tGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTa                                     <  1:69538/126‑1 (MQ=255)

                                                                                                         tGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTa                                     >  2:69538/1‑126 (MQ=255)

                                                                                                         tGCCGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCt                       >  2:635870/1‑140 (MQ=255)

                                                                                                            cGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgcg                    <  2:479768/140‑1 (MQ=255)

                                                                                                            cGGGTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgcg                    <  1:635870/140‑1 (MQ=255)

                                                                                                             gggTTTTCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTgcgc                   <  1:671193/140‑1 (MQ=255)

                                                                                                                 tttCGAGAAAACGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCAca               <  2:207136/140‑2 (MQ=255)

                                                                                                                          aaCGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTa      >  2:508058/1‑140 (MQ=255)

                                                                                                                           aCGCGCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAg     <  2:970777/140‑1 (MQ=255)

                                                                                                                            cgcgCCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGc    <  2:461296/140‑1 (MQ=255)

                                                                                                                              cgcCAGCCCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCaa  <  2:820496/140‑1 (MQ=255)

CGGACCTGCGCGTTCACGCAAGGCGGCAAAGAAAGGATAACGGCGATAATGCAGGCCACTGCCGGTATGGCCTGCCTGCCAGCTCGCCGCCAGATAACGCGGATCTGCCGGGTTTTCGAGAAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGTTAGCAA  >  NC_000913/3561168‑3561433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A