Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	172	67.8	2301510	98.9%	2276193	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,566,281	C→T	A101T (GCA→ACA)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,281	0	C	T	100.0%	126.1 / NA	36	A101T (GCA→ACA)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/19); total (17/19)

CCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAG  >  NC_000913/3566145‑3566414

ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    <  1:539922/140‑1 (MQ=255)

   cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    >  1:1108333/1‑137 (MQ=255)

   cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGcc                                                                                                                                    <  2:1108333/137‑1 (MQ=255)

   cgcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTg                                                                                                                                 <  1:1103771/140‑1 (MQ=255)

             aCCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCg                                                                                                                       <  1:663757/140‑1 (MQ=255)

                         ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGc                                                                                                           >  1:345101/1‑140 (MQ=255)

                           agagAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGa                                                                                                         <  1:560698/140‑1 (MQ=255)

                                 tCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACAt                                                                                                   >  2:309339/1‑140 (MQ=255)

                                  cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                                                                                  <  2:906377/140‑1 (MQ=255)

                                         gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                                                                           <  2:382028/140‑1 (MQ=255)

                                                 ccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTa                                                                                   >  2:451176/1‑140 (MQ=255)

                                                        tCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCaa                                                                            >  1:400190/1‑140 (MQ=255)

                                                                   cGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                                                                 >  1:116335/1‑140 (MQ=255)

                                                                       aCCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg                                                             >  2:30205/1‑140 (MQ=255)

                                                                          gAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa                                                          <  2:838709/140‑1 (MQ=255)

                                                                              ccTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta                                                      >  1:1013669/1‑140 (MQ=255)

                                                                                 gggTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgtagta                                                   <  1:451176/140‑1 (MQ=255)

                                                                                  ggTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa                                                  >  1:120973/1‑140 (MQ=255)

                                                                                             tcatcaATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGt                                       >  1:871081/1‑140 (MQ=255)

                                                                                              catcaATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGtt                                      >  2:800300/1‑140 (MQ=255)

                                                                                                    tcaGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGc                                <  2:120973/140‑1 (MQ=255)

                                                                                                       gCTCTTCGAGATTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGAc                             <  1:590028/140‑1 (MQ=255)

                                                                                                              gagaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTggg                      <  1:63638/140‑1 (MQ=255)

                                                                                                              gagaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTggg                      >  1:681044/1‑140 (MQ=255)

                                                                                                                gaTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCa                    <  2:400190/140‑1 (MQ=255)

                                                                                                                 aTTTAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCAc                   <  2:116335/140‑1 (MQ=255)

                                                                                                                    tAACCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGt                <  1:800300/140‑1 (MQ=255)

                                                                                                                     aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               <  2:681044/140‑1 (MQ=255)

                                                                                                                     aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               >  2:244838/1‑140 (MQ=255)

                                                                                                                     aaCCCCATCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGtt               <  1:1075625/140‑1 (MQ=255)

                                                                                                                           aTCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACg         >  1:1038760/1‑140 (MQ=255)

                                                                                                                            tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  2:146051/1‑140 (MQ=255)

                                                                                                                            tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  1:992564/1‑140 (MQ=255)

                                                                                                                            tCGCTTCCAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGt        >  2:923848/1‑140 (MQ=255)

                                                                                                                                  ccAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAg  <  1:244838/140‑1 (MQ=255)

                                                                                                                                  ccAGTGTGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAg  <  1:146051/140‑1 (MQ=255)

CCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAG  >  NC_000913/3566145‑3566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A